本文選題：鼻咽腫瘤 + 伴有kazal域的富含半胱氨酸的逆轉(zhuǎn)誘導(dǎo)蛋白�。� 參考：《福建醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的檢測(cè)永生化鼻咽上皮細(xì)胞系NP69-SV40T和具有不同轉(zhuǎn)移傾向的鼻咽癌細(xì)胞系SUNE-1亞株-6-10B(只成瘤,不轉(zhuǎn)移)、SUNE-1亞株-5-8F(高成瘤,高轉(zhuǎn)移)中,伴有kazal域的富含半胱氨酸的逆轉(zhuǎn)誘導(dǎo)蛋白(reversion-inducing-cysteine-rich protein with kazal motifs,RECK)、高級(jí)糖基化終末產(chǎn)物受體(receptors for advanced glycation end products)及基質(zhì)金屬蛋白酶9(matrix metalloproteinase 9,MMP-9)的mRNA表達(dá)情況。 方法應(yīng)用實(shí)時(shí)熒光定量PCR技術(shù)檢測(cè)永生化鼻咽上皮細(xì)胞系NP69-SV40T、鼻咽癌細(xì)胞系SUNE-1亞株-6-10B、SUNE-1亞株-5-8F中RECK、RAGE及MMP-9 mRNA的表達(dá)。 結(jié)果MMP-9 mRNA在鼻咽癌細(xì)胞系中的表達(dá)較永生化鼻咽上皮細(xì)胞系NP69-SV40T下調(diào)(P0.05),鼻咽癌細(xì)胞系5-8F組表達(dá)稍高于6-10B組,兩者間差異無顯著性(P0.05);RAGE mRNA在鼻咽癌細(xì)胞系中的表達(dá)較永生化鼻咽上皮細(xì)胞系NP69-SV40T稍下調(diào),三種細(xì)胞系中的表達(dá)差異無顯著性(P0.05);RECK mRNA在三種細(xì)胞系中的表達(dá)差異亦無顯著性(P0.05)。 結(jié)論體外條件下RAGE、RECK和MMP-9 mRNA的表達(dá)水平與細(xì)胞系性質(zhì)無明顯關(guān)聯(lián),與NPC細(xì)胞系潛在的轉(zhuǎn)移能力無明顯關(guān)聯(lián),三者之間亦無顯著相關(guān)。 目的檢測(cè)伴有kazal域的富含半胱氨酸的逆轉(zhuǎn)誘導(dǎo)蛋白(reversion-inducing-cysteine-rich protein with kazal motifs, RECK)、高級(jí)糖基化終末產(chǎn)物受體(receptors for advanced glycation end products, RAGE)及基質(zhì)金屬蛋白酶9(matrix metalloproteinase 9, MMP-9)在鼻咽癌(nasopharyngeal carcinoma, NPC)組織中的表達(dá)情況及其相關(guān)性。 方法運(yùn)用免疫組化方法檢測(cè)64例NPC組織(伴頸淋巴結(jié)轉(zhuǎn)移者30例,無頸淋巴結(jié)轉(zhuǎn)移者34例)和30例慢性鼻咽炎組織(chronic nasopharyngitis tissue, CNT)中RECK、RAGE和MMP-9蛋白的表達(dá)。 結(jié)果RECK在CNT及NPC組織表達(dá)陽性率極低,且兩者間差異無顯著性(P 0.05),但周邊炎癥細(xì)胞及癌巢周邊基質(zhì)中RECK強(qiáng)表達(dá),在伴與不伴頸淋巴結(jié)轉(zhuǎn)移組中的陽性率分別為3.3%(1/30)和11.8%(4/30),兩者間差異無顯著性(P 0.05);RAGE表達(dá)陽性率在CNT和NPC組織分別為100% (30/30)和70.3% (45/64),MMP-9則分別為46.7%(14/30)和78.1% (50/64);RAGE在伴與不伴頸淋巴結(jié)轉(zhuǎn)移組中的陽性率分別為86.7%(26/30)和55.9%(19/34),MMP-9則分別為90.0%(27/30)和67.7%(23/34),各組間差異均具統(tǒng)計(jì)學(xué)意義(P0.05)。在NPC組織中,RECK與MMP-9的表達(dá)呈負(fù)相關(guān)(r=-0.369,p0.01), RAGE與MMP-9則呈正相關(guān)(r=0.471,p0.01)。 結(jié)論RECK、RAGE在NPC組織較CNT中表達(dá)下調(diào),MMP-9則上調(diào);在伴頸淋巴結(jié)轉(zhuǎn)移的NPC組織中,RECK表達(dá)下調(diào),RAGE、MMP-9的表達(dá)上調(diào)。三者的異常表達(dá)可能與NPC的進(jìn)展有關(guān),RECK、RAGE可能通過調(diào)節(jié)MMP-9的表達(dá)參與NPC的侵襲轉(zhuǎn)移過程。
[Abstract]:Objective to detect the relationship between immortalized nasopharyngeal epithelial cell line NP69-SV40T and nasopharyngeal carcinoma cell line SUNE-1 substrain -6-10B (tumorigenic, non-metastatic) substrain -5-8F (Gao Cheng tumor, high metastasis). MRNA expression of cysteine-rich reverse inducible protein (reversion-inducing-cysteine-rich protein with kazal motifs), advanced glycosylation end product receptor (receptors for advanced glycation end products) and matrix metalloproteinase-9 (matrix metalloproteinase 9 (MMP-9) with kazal domain. Methods Real-time quantitative PCR was used to detect the mRNA expression of RECKnRAGE and MMP-9 in immortalized nasopharyngeal epithelial cell line NP69-SV40T and nasopharyngeal carcinoma cell line SUNE-1 substrain -6-10BU SUNE-1 substrain -5-8F. Results the expression of MMP-9 mRNA in nasopharyngeal carcinoma cell line was lower than that in immortalized nasopharyngeal epithelial cell line NP69-SV40T (P0.05). The expression of MMP-9 mRNA in nasopharyngeal carcinoma cell line 5-8F was slightly higher than that in 6-10B group. The expression of rage mRNA in nasopharyngeal carcinoma cell line was lower than that in immortalized nasopharyngeal epithelial cell line NP69-SV40T (P0.05). Conclusion there is no significant correlation between the expression level of rack and MMP-9 mRNA in vitro and the nature of the cell line, and the potential metastasis ability of NPC cell line, and there is no significant correlation between them. Objective to investigate the expression of cysteine-rich reverse inducible protein (reversion-inducing-cysteine-rich protein with kazal motifs, reck), advanced glycation end product receptor (receptors for advanced glycation end products, rage) and matrix metalloproteinase-9 (MMP-9) in nasopharyngeal carcinoma (NPC) tissues with kazal domain. Reach the situation and its relevance. Methods Immunohistochemical method was used to detect the expression of RECK rage and MMP-9 protein in 64 NPC tissues (30 cases with cervical lymph node metastasis, 34 cases without cervical lymph node metastasis) and 30 cases with chronic nasopharyngitis. Results the positive rate of reck in CNT and NPC tissues was very low, and there was no significant difference between them (P 0.05). The positive rates of rage were 3.3% (1 / 30) and 11.8% (4 / 30) in patients with and without cervical lymph node metastasis, respectively. There was no significant difference between them (P 0.05). The positive rates of rage expression in CNT and NPC tissues were 100% (30 / 30) and 70.3% (45 / 64), respectively, 46.7% (14 / 30) and 78.1% (50 / 64) in CNT and NPC tissues. The positive rates of MMP-9 in metastatic group were 86.7% (26 / 30) and 55.9% (19 / 34), respectively. The positive rates of MMP-9 in metastatic group were 90.0% (27 / 30) and 67.7% (23 / 34), respectively (P0.05). In NPC tissues, the expression of reck was negatively correlated with MMP-9 (r-0.369, p0.01), while rage was positively correlated with MMP-9 (r-0.471p0.01). Conclusion the down-regulated expression of MMP-9 was up-regulated in NPC tissues compared with CNT, and down-regulated in NPC with cervical lymph node metastasis, and the expression of RAGE-MMP-9 was up-regulated in NPC tissues with cervical lymph node metastasis. The abnormal expression of these three proteins may be related to the progression of NPC, which may be involved in the invasion and metastasis of NPC by regulating the expression of MMP-9.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R739.63

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