本文選題：光照強度 + 屈光發(fā)育　； 參考：《復旦大學》2011年碩士論文

【摘要】：目的：探討豚鼠在不同光強度照明條件下(5Lux,500Lux,10000 Lux,白色光,色溫6000K)的屈光發(fā)育狀況,以比較光強度對豚鼠屈光發(fā)育的影響,并檢測視網(wǎng)膜多巴胺含量變化,初步探討光照強度影響屈光發(fā)育的可能機制。 方法：30只3周齡的健康豚鼠(英國種三色豚鼠),隨機分為強光組10只、弱光組10只和正常對照組10只,分別置于10000Lux,5Lux,500Lux三種光照強度下,光照周期為12/12h(早6：00-晚6：00)。于實驗前及光照12周末分別用帶狀檢影計、A超測定儀、角膜曲率計對豚鼠右眼重復進行眼球的生物學測量(包括屈光度、眼軸、角膜曲率)。光照12周結(jié)束后處死豚鼠取右眼球行高效液相色譜(HPLC)分析以及光鏡電鏡下行后極眼球壁結(jié)構(gòu)觀察。對不同時間點的組間測量數(shù)據(jù)采用單因素方差分析,以P0.05為差異有統(tǒng)計學意義。 結(jié)果：光照前不同組間生物學測量參數(shù)差異無顯著性(P0.05)。光照12周后,強光組屈光度為4.03±1.59D,同光照前相比發(fā)生0.45±1.65D的變化,正常對照組屈光度為2.15±2.O1D,發(fā)生2.28±0.66D的變化,強光組同正常對照組相比遠視度數(shù)偏高約1.501),兩者差異有顯著性(P0.05)；強光組眼軸增長0.54±0.10mm,正常對照組為0.76±0.05mm,強光組較對照組眼軸長度延長較慢,差異有顯著性(P0.05)；光照后不同組角膜曲率半徑均增加,但組間變化差異無顯著性(P0.05)。強光組視網(wǎng)膜多巴胺含量平均為148.70±22.44 nmol/g,正常對照組為44.50±12.45 nmol/g,兩者差異有顯著性(P0.01)。光照12周后弱光組較正常對照組相比,無論是屈光度、曲率、眼軸以及視網(wǎng)膜多巴胺含量均無統(tǒng)計學差異(P0.05)。光鏡下見強光組后極部鞏膜層較正常光照組厚。電鏡下見強光組光感受器部分外節(jié)膜盤間隙增寬,內(nèi)節(jié)部分線粒體腫脹,嵴脫落,余未見明顯異常。 結(jié)論：高強度光照可以引起豚鼠眼球眼軸增長減緩,正視化進展減慢,屈光度數(shù)偏遠視,弱光對豚鼠的屈光發(fā)育沒有影響。強光照射后可以引起豚鼠視網(wǎng)膜多巴胺含量增加,可能為強光引起豚鼠正視化進展減緩的機制之一。強光對豚鼠視網(wǎng)膜光感受器造成輕微損傷。
[Abstract]:Objective: to study the refractive development of guinea pigs under different light intensity illumination (5Lux500Lux10000 Lux10000, white light, color temperature 6000K), to compare the effect of light intensity on refractive development of guinea pigs, and to detect the changes of dopamine content in retina. To explore the possible mechanism of light intensity affecting refractive development. Methods Thirty 3-week-old healthy guinea pigs (British tricolor guinea pigs) were randomly divided into three groups: high light group (n = 10), weak light group (n = 10) and normal control group (n = 10). Under three light intensities of 10000Lux5Lux500Lux, the period of illumination was 12 / 12h (6: 00 to 6:00). The biologic measurements of the right eye of guinea pig (including diopter, axis, cornea curvature) were carried out before the experiment and at the end of 12 weeks of illumination by using the band detector and keratometer respectively. At the end of 12 weeks of illumination, the right eye balls of the guinea pigs were sacrificed for HPLC analysis and for observation of the wall structure of the posterior polar eyeball under light microscope and electron microscope. Single factor analysis of variance (ANOVA) was used to measure the data at different time points, and the difference was statistically significant (P0.05). Results: there was no significant difference in biological parameters between different groups before illumination (P0.05). After 12 weeks of illumination, the diopter of the high-light group was 4.03 鹵1.59D, which was 0.45 鹵1.65D compared with that of the control group, and the diopter of the control group was 2.15 鹵2.O1D and 2.28 鹵0.66D, and the hyperopia degree of the high-light group was higher than that of the control group (about 1.501), the difference was significant (P0.05). The axial length of the eyes in the strong light group was 0.54 鹵0.10 mm, and that in the normal control group was 0.76 鹵0.05 mm. The length of the axial axis in the strong light group was longer than that in the control group (P0.05), and the corneal radius of curvature was increased in the different groups after illumination (P0.05). The average dopamine content was 148.70 鹵22.44 nmol / g in the high-light group and 44.50 鹵12.45 nmol / g in the normal control group. There was a significant difference between the two groups (P0.01). There was no significant difference in diopter curvature eye axis and retinal dopamine content between the low light group and the normal control group after 12 weeks of illumination (P0.05). Under light microscope, the posterior sclera of the strong light group was thicker than that of the normal light group. Under the electron microscope, the outer segment of the photoreceptor in the strong light group was enlarged, the mitochondria of the inner ganglia was swollen, the ridge was abscission, and no obvious abnormality was found in the rest. Conclusion: high intensity illumination can slow down the growth of eye axis, slow down the progress of emmetropia, and have no effect on the development of refraction in guinea pigs. The dopamine content in the retina of guinea pigs increased after strong light irradiation, which may be one of the mechanisms of slowing down the progress of emmetropia caused by intense light. Strong light caused slight damage to retinal photoreceptors in guinea pigs.
【學位授予單位】：復旦大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R778

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