本文選題：人 + 鼻腔�。� 參考：《臨床耳鼻咽喉頭頸外科雜志》2014年12期

【摘要】：目的:浸沒培養(yǎng)法培養(yǎng)人鼻腔纖毛上皮細胞,為纖毛相關(guān)研究及經(jīng)鼻藥物安全性評價等研究提供可靠的細胞學模型。方法:采用低溫酶消化法,浸沒培養(yǎng)人鼻腔纖毛上皮細胞,倒置相差顯微鏡觀察細胞生長狀況,掃描電鏡及免疫細胞化學方法觀察細胞融合及纖毛分化狀態(tài),高速數(shù)字化顯微視頻成像系統(tǒng)檢測纖毛擺動頻率。結(jié)果:①相差顯微鏡下,纖毛細胞數(shù)量逐漸增加,至7~10d達到高峰后逐漸減少,纖毛細胞存活時間維持在14~21d;②細胞培養(yǎng)第7天,掃描電鏡可見鼻腔上皮細胞表面覆蓋纖毛或微絨毛,杯狀細胞及無纖毛柱狀上皮細胞相間排列;③細胞培養(yǎng)第7天,Ⅳ型β-微管蛋白、閉合小環(huán)蛋白-1免疫熒光結(jié)果顯示細胞融合及纖毛分化良好,纖毛細胞比例可達20%~30%;④培養(yǎng)第7、14、21天纖毛擺動基礎(chǔ)頻率分別為(10.73±2.15)Hz、(9.92±1.97)Hz、(10.30±2.11)Hz,無明顯統(tǒng)計學差異;⑤外源性刺激劑100μmol/L ATP可明顯增加纖毛擺動頻率。結(jié)論:應(yīng)用酶消化法浸沒培養(yǎng)人鼻腔纖毛上皮細胞,細胞融合及纖毛分化狀態(tài)良好,纖毛擺動活躍,對外源性刺激反應(yīng)靈敏,是應(yīng)用于纖毛相關(guān)研究及經(jīng)鼻藥物安全性評價等研究的一個較為理想的細胞模型。
[Abstract]:Objective: to culture human nasal ciliated epithelial cells by immersion culture, and to provide a reliable cytological model for the study of cilium and safety evaluation of transnasal drugs. Methods: human nasal ciliated epithelial cells were incubated by low temperature enzyme digestion. The growth status of cells was observed by inverted phase contrast microscope, and cell fusion and ciliated differentiation were observed by scanning electron microscope and immunocytochemistry. High-speed digital micro-video imaging system detects cilium swinging frequency. Results under the microscope, the number of ciliated cells increased gradually, and then decreased gradually after reaching the peak at day 7. The survival time of ciliated cells was maintained at 1421 days after 7 days of culture. Scanning electron microscopy showed that nasal epithelial cells were covered with ciliated or microvilli, goblet cells and non-ciliated columnar epithelial cells were arranged on the 7th day of culture, and type 鈪，

本文編號：2048828