本文選題：棘阿米巴角膜炎 + 動物模型�。� 參考：《福建醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的建立一種模擬臨床人角膜感染棘阿米巴的動物模型,對其角膜組織損傷的臨床表現(xiàn)和組織病理學(xué)改變進(jìn)行觀察,同時(shí)探討角膜棘阿米巴原蟲感染后角膜炎癥細(xì)胞因子MCP-1、IL-1β的表達(dá)。 方法40只新西蘭白兔,應(yīng)用角膜表面鏡片術(shù)法,即刮除角膜上皮,覆蓋角膜植片,二者層間注入棘阿米巴滋養(yǎng)體混懸液,縫合眼瞼24h,建立棘阿米巴角膜炎模型,并觀察角膜潰瘍形態(tài),行角膜HE染色或PAS染色組織病理切片檢查,并應(yīng)用逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)技術(shù),檢測不同病程角膜組織中的IL-1β、MCP-l的表達(dá)。 結(jié)果40只兔子右眼均感染棘阿米巴性角膜炎,并經(jīng)角膜刮片及培養(yǎng)、組織病理切片染色檢查證實(shí)。感染眼角膜出現(xiàn)水腫,環(huán)形基質(zhì)浸潤,角膜新生血管等表現(xiàn);其病理組織切片上可見基質(zhì)層內(nèi)的棘阿米巴滋養(yǎng)體或包囊,伴有中性粒細(xì)胞,單核巨噬細(xì)胞,嗜酸性粒細(xì)胞等的浸潤。實(shí)驗(yàn)組右眼病變角膜組織中,IL-1β含量于感染后第1天、第3天、第5天均有大量表達(dá),于感染后第3天表達(dá)量達(dá)到高峰,第7天后呈現(xiàn)逐漸下降趨勢,與角膜炎癥程度趨勢一致(P0.01);MCP-l含量于第1天、第3天、第5天、第7天和第9天均明顯升高,于感染后第5天表達(dá)量達(dá)最高值,以后逐漸下降(P0.01)。 結(jié)論應(yīng)用角膜表面鏡片法可以成功建立兔棘阿米巴性角膜炎模型,可應(yīng)用于棘阿米巴角膜炎的免疫學(xué)機(jī)制研究,IL-1β可能是兔棘阿米巴性角膜炎角膜炎癥反應(yīng)程度的敏感指標(biāo);而MCP-1則可能是兔棘阿米巴角膜炎中重要的防御和保護(hù)性因素。
[Abstract]:Objective to establish an animal model of Acanthamoeba infection in human cornea and observe the clinical manifestations and histopathological changes of corneal tissue injury. At the same time, the expression of inflammatory cytokine MCP-1and IL-1 尾 in cornea after infection of amoeba cornea was studied. Methods 40 New Zealand white rabbits were treated with keratoscopy, namely, the corneal epithelium was scraped, the corneal graft was covered, the suspension of trophozoites of Acanthamoeba was injected into the two layers, the eyelid was sutured for 24 hours, and the model of Acanthamoeba keratitis was established. The morphology of corneal ulcer was observed and the expression of IL-1 尾 -MCP-l was detected by reverse transcriptase polymerase chain reaction (RT-PCR) technique. Results Acanthoamoeba keratitis was infected in the right eyes of 40 rabbits and was confirmed by corneal scraping and culture. Edema of the infected cornea, infiltration of circular stroma, corneal neovascularization and so on. The trophozoites or cysts of Acanthamoeba in the stroma layer were found in the pathological sections, with neutrophils and mononuclear macrophages. Infiltration of eosinophils, etc. In the experimental group, the expression of IL-1 尾 in the cornea of the right eye lesion reached its peak on the third day after infection, and decreased gradually after 7 days, after infection, and on the first day, the third day and the fifth day after infection, and the expression of IL-1 尾 in the cornea of the experimental group reached its peak on the third day after infection. In accordance with the tendency of corneal inflammation, the content of MCP-l increased significantly on the 1st, 3rd, 5th, 7th and 9th day, and reached the highest level on the 5th day after infection, and then decreased gradually. Conclusion the rabbit model of Acanthamoeba keratitis can be successfully established by using corneal surface lens method, and the immunological mechanism of Acanthamoeba keratitis can be studied. IL-1 尾 may be a sensitive index for the degree of inflammation of rabbit Acanthamoeba cornea. MCP-1 may be an important defensive and protective factor in acanthoamoeba keratitis in rabbits.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R772.21

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