本文選題：Corti氏器 + 螺旋神經(jīng)節(jié)細(xì)胞��； 參考：《中南大學(xué)》2010年博士論文

【摘要】：耳蝸毛細(xì)胞與螺旋神經(jīng)節(jié)細(xì)胞是維持正常聽(tīng)力的關(guān)鍵細(xì)胞。耳蝸缺血、缺氧、噪聲損傷和耳毒性藥物等傷害性因素可對(duì)它們?cè)斐刹煌潭鹊膿p傷而引起感音神經(jīng)性耳聾,后者是導(dǎo)致聽(tīng)力殘疾的重要原因,且發(fā)病率較高,目前仍無(wú)有效治療手段。 眾多研究證實(shí),多種神經(jīng)營(yíng)養(yǎng)因子如神經(jīng)營(yíng)養(yǎng)素3(Neurotrophin 3,NT-3)對(duì)早期感音神經(jīng)性耳聾有一定的療效。但由于神經(jīng)營(yíng)養(yǎng)因子昂貴且在體內(nèi)容易降解,加之耳蝸是一個(gè)高度精密的密閉的電生理器官,不宜反復(fù)進(jìn)行有創(chuàng)性給藥,一次性給藥后即能維持較長(zhǎng)時(shí)間的基因治療方式就成為其首選。另一方面,由于缺乏理想的安全、高效的基因載體和合適的基因轉(zhuǎn)染途徑,內(nèi)耳基因治療研究迄今仍停留在實(shí)驗(yàn)室階段,一些有關(guān)基因轉(zhuǎn)染后分布、代謝、轉(zhuǎn)染效率和生物安全性等問(wèn)題,仍需進(jìn)行大量的體外研究。 本研究選用新生C57/BL-6J小鼠,對(duì)耳蝸螺旋神經(jīng)節(jié)細(xì)胞與Corti器進(jìn)行原代培養(yǎng)；以新型脂質(zhì)體LipofectamineTM LTXPLUS為載體向靶細(xì)胞與靶器官轉(zhuǎn)染NT-3基因,研究目的基因在螺旋神經(jīng)節(jié)細(xì)胞與Corti器的表達(dá)及其對(duì)耳蝸螺旋神經(jīng)節(jié)細(xì)胞生長(zhǎng)的影響；同時(shí)研究NT-3基因的表達(dá)產(chǎn)物對(duì)慶大霉素所致螺旋神經(jīng)節(jié)細(xì)胞與Corti器毛細(xì)胞損傷的保護(hù)作用,為內(nèi)耳基因治療提供實(shí)驗(yàn)依據(jù)。
[Abstract]:cochlear hair cells and spiral ganglion cells are the key cells in the maintenance of normal hearing . Injury factors such as cochlear ischemia , hypoxia , noise injury and ototoxic drugs can cause sensorineural deafness due to different degrees of injury , which is an important cause of hearing disability , and has a higher incidence , and there is still no effective treatment .

Many studies have shown that various neurotrophic factors , such as neurotrophin 3 ( NT - 3 ) , have a certain therapeutic effect on early sensorineural deafness . However , due to the fact that the neurotrophic factor is expensive and easy to degrade in vivo , the cochlear is a highly precise closed electrophysiologic organ .

In this study , newborn C57 / BL - 6J mice were selected for primary culture of cochlear spiral ganglion cells and Corti .
The NT - 3 gene was transfected into target cells and target organs by Lipofectamine TM LTXPLUS . The expression of target gene in spiral ganglion cells and Corti and its effect on the growth of cochlear spiral ganglion cells were studied .
At the same time , the protective effect of the expression product of NT - 3 gene on the damage of spiral ganglion cells and Corti ' s hair cells caused by gentamicin was studied , which provided experimental basis for the gene therapy of inner ear .
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2010
【分類號(hào)】：R764

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