本文選題：降鈣素基因相關(guān)肽 + 三叉神經(jīng)節(jié)��； 參考：《華中科技大學(xué)》2011年碩士論文

【摘要】：目的:觀察大鼠三叉神經(jīng)節(jié)內(nèi)降鈣素基因相關(guān)肽(Calcitonin gene-related peptide CGRP)陽(yáng)性細(xì)胞的分布情況,及其對(duì)眼部組織的支配情況,初步了解CGRP與眼部痛覺產(chǎn)生及調(diào)控的相關(guān)性。 方法:隨機(jī)抽取5只SD大鼠,10只眼,隨機(jī)分為生理鹽水對(duì)照組,上眼瞼+球結(jié)膜注射組,球結(jié)膜注射組,上眼瞼注射組,前房注射組。將生理鹽水,熒光金分組注入大鼠球結(jié)膜,上眼瞼及前房,逆行示蹤三叉神經(jīng)節(jié)神經(jīng)元,同時(shí)用CGRP免疫熒光組織化學(xué)標(biāo)記大鼠三叉神經(jīng)節(jié)組織。在熒光顯微鏡下統(tǒng)計(jì)分析熒光金標(biāo)記、CGRP免疫熒光分別標(biāo)記及雙重標(biāo)記的三叉神經(jīng)節(jié)神經(jīng)元數(shù)量。采用SPSS 13.0統(tǒng)計(jì)軟件對(duì)所得數(shù)據(jù)進(jìn)行t檢驗(yàn)。 結(jié)果:通過熒光金逆行示蹤的方法,球結(jié)膜,上眼瞼及前房分別注射組均發(fā)現(xiàn)在三叉神經(jīng)節(jié)內(nèi)可見熒光金標(biāo)記的神經(jīng)元,但在數(shù)量上存在一定的差異;運(yùn)用免疫熒光組織化學(xué)染色的方法,三叉神經(jīng)節(jié)內(nèi)可見許多CGRP陽(yáng)性神經(jīng)元;兩種方法相結(jié)合可以觀察到少量熒光金標(biāo)記的神經(jīng)元同時(shí)也呈CGRP免疫熒光陽(yáng)性。對(duì)標(biāo)記的神經(jīng)元計(jì)數(shù),進(jìn)行統(tǒng)計(jì)學(xué)分析,支配眼內(nèi)組織感覺的神經(jīng)元數(shù)量與支配球結(jié)膜及上眼瞼的數(shù)量進(jìn)行對(duì)比,P0.05,差異有統(tǒng)計(jì)學(xué)意義。 結(jié)論:三叉神經(jīng)節(jié)內(nèi)有大量CGRP免疫陽(yáng)性神經(jīng)元支配眼部組織,其中有少量神經(jīng)元支配眼內(nèi)組織,CGRP參與眼部傷害性刺激的調(diào)控。
[Abstract]:Objective: To observe the distribution of Calcitonin gene-related peptide CGRP positive cells in the trigeminal ganglion of the rat trigeminal ganglia, and the control of the ocular tissue, and to understand the correlation between CGRP and the production and regulation of ocular pain.
Methods: 5 SD rats were randomly selected, 10 eyes were randomly divided into normal saline control group, upper eyelid + conjunctival injection group, ball conjunctiva injection group, upper eyelid injection group and anterior chamber injection group. The saline and fluorescent gold groups were injected into the rat conjunctiva, upper eyelid and anterior chamber, and the trigeminal ganglion neurons were traced by inverse line, and CGRP immunofluorescence tissue was used. The trigeminal ganglion tissue of rats was marked by chemical labeling. The fluorescent gold labeling, CGRP immunofluorescence and the number of double labeled trigeminal ganglion neurons were statistically analyzed under the fluorescence microscope. The data were tested by the SPSS 13 statistical software for the t test.
Results: by the method of retrograde tracing of fluorescent gold, the neurons in the trigeminal ganglia were found in the conjunctiva, the upper eyelids and the anterior chamber respectively, but there were some differences in the number of neurons. Many CGRP positive neurons were seen in the trigeminal ganglion by immunofluorescence staining, and the two methods were found in the trigeminal ganglion. A small amount of fluorescent gold labeled neurons were also observed to be immunofluorescent with CGRP immunofluorescence. The count of labeled neurons was statistically analyzed, and the number of neurons that dominate the sensory tissue of the eye was compared with the amount of the domination of the conjunctiva and the upper eyelids, and the difference was statistically significant in P0.05.
Conclusion: there are a large number of CGRP immunoreactive neurons in the trigeminal ganglia, which dominate the ocular tissue, and a small number of neurons dominate the intraocular tissue, and CGRP is involved in the regulation of ocular nociceptive stimulation.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R77

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