本文選題：糖尿病視網(wǎng)膜病變 + 血管內(nèi)皮生長(zhǎng)因子�。� 參考：《眼科新進(jìn)展》2014年07期

【摘要】：目的探討增殖性糖尿病視網(wǎng)膜病變(proliferative diabetic retinopathy,PDR)患者血漿、房水、玻璃體中血管內(nèi)皮生長(zhǎng)因子(vascular endothelial growth factor,VEGF)與組織纖溶酶原激活物(tissue type plasminogen activator,t-PA)及t-PA抑制劑(plasminogen activator inhibitor,PAI)的表達(dá),并評(píng)價(jià)VEGF表達(dá)與t-PA、PAI的相關(guān)性。方法采用雙抗體夾心酶聯(lián)免疫吸附試驗(yàn)(ELISA)法定量檢測(cè)來(lái)自寧夏醫(yī)科大學(xué)總醫(yī)院眼科的PDR組及對(duì)照組患者血漿、房水、玻璃體中VEGF、t-PA、PAI的含量。結(jié)果 PDR組血漿中VEGF(63.12±16.25)pg·mL-1、PAI(10.27±7.62)ng·mL-1的表達(dá)均高于對(duì)照組(14.39±10.48)pg·mL-1、(0.28±0.11)ng·mL-1,差異均有統(tǒng)計(jì)學(xué)意義(均為P0.051;PDR組血漿中t-PA(19.91±9.90)ng·mL-1與對(duì)照組(12.00±2.61)ng·mL-1比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.01)。PDR組房水中VEGF(291.21±45.04)pg·mL-1、t-PA(5.38±2.40)ng·mL-1、PAI(0.53±0.31)ng·mL-1均高于對(duì)照組(30.60±11.70)pg·mL-1、(3.87±1.32)ng·mL-1、(0.16±0.09)ng·mL-1,差異均有統(tǒng)計(jì)學(xué)意義(均為P0.05);PDR組玻璃體中VEGF(527.62±79.00)pg·mL-1、t-PA(10.41±6.33)ng·mL-1、PAI(7.72±2.32)ng·mL-1的表達(dá)濃度均高于對(duì)照組(37.58±8.31)pg·mL-1、(3.68±2.48)ng·mL-1、(0.27±0.13)ng·mL-1,差異均有統(tǒng)計(jì)學(xué)意義(均勻P0.05)。PDR組自身血漿、房水、玻璃體三者比較,VEGF表達(dá):玻璃體房水血漿;t-PA表達(dá):血漿玻璃體房水;PAI表達(dá):血漿、玻璃體均高于房水,但血漿和玻璃體比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。PDR組血漿、房水、玻璃體中VEGF的表達(dá)與t-PA及PAI的表達(dá)均存在顯著相關(guān)性(均為P0.05)。結(jié)論 VEGF、t-PA、PAI可能參與了PDR視網(wǎng)膜新生血管的發(fā)生過(guò)程;VEGF與t-PA、PAI在PDR的發(fā)展中可能具有相互促進(jìn)的作用。
[Abstract]:Objective to investigate the expression of vascular endothelial growth factor (VEGF) in plasma, aqueous humor and vitreous of proliferative diabetic retinopathy (PDR) patients with proliferative diabetic retinopathy (PDR), tissue type plasminogen activator t-PAI and plasminogen activator inhibitor PAI. To evaluate the correlation between VEGF expression and t-PAP PAI. Methods double antibody sandwich enzyme linked immunosorbent assay (Elisa) was used to determine the content of VEGFT-PAP PAI in plasma, aqueous humor and vitreous of PDR patients from ophthalmological hospital of Ningxia Medical University. 緇撴灉 PDR緇勮嫻嗕腑VEGF(63.12鹵16.25)pg路mL-1,PAI(10.27鹵7.62)ng路mL-1鐨勮〃杈懼潎楂樹(shù)簬瀵圭収緇，

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