本文選題：鼻內(nèi)翻性乳頭狀瘤 + FSCNI��； 參考：《鄭州大學(xué)》2011年碩士論文

【摘要】：背景與目的 鼻內(nèi)翻性乳頭狀瘤(nasal inverted papilloma, NIP)是耳鼻咽喉頭頸外科比較多見的鼻腔鼻竇良性腫瘤,約占鼻腔鼻竇腫瘤的0.4%-4.7%,其男女性別發(fā)病率差別較大,男性顯著高于女性,城市高于農(nóng)村。據(jù)有關(guān)報道顯示,隨著人類受飲食,生活及周邊環(huán)境的影響,具有侵襲性、高復(fù)發(fā)性和易癌變等臨床特點。其病因復(fù)雜且誘發(fā)因素較多,迄今確切的病因尚不清楚,可能為多種因素綜合作用所致。隨著對信號通路傳導(dǎo)系統(tǒng)在腫瘤分子生物學(xué)領(lǐng)域的研究,通過干預(yù)信號通路傳導(dǎo)途徑治療腫瘤已成為生物治療的新興領(lǐng)域。 Fascin蛋白屬于三葉蟲結(jié)構(gòu)蛋白家族中的一員,人類Fascin-1 (FSCN1)基因定位于染色體7p22,在正常組織器官中,FSCN1表達于間葉組織和神經(jīng)系統(tǒng),編碼一種分子質(zhì)量為55 kDa的細胞骨架蛋白,可與F-肌動蛋白結(jié)合,定位于細胞質(zhì)張力纖維和細胞膜皺褶邊緣的絲狀偽足、微棘的核心肌動蛋白束中,Fasein蛋白N端第39位的絲氨酸為蛋白激酶C(PKC)的磷酸化位點,該位點的磷酸化可調(diào)節(jié)Fascin蛋白與F-actin的結(jié)合活性以及細胞膜表面絲狀偽足和微棘的形成,提示Fascin蛋白可能在細胞遷移、細胞黏附以及細胞間信息交流等過程中發(fā)揮作用,在腫瘤的形成和發(fā)展中扮演著重要的角色,促進腫瘤的侵襲和轉(zhuǎn)移。 肝細胞生長因子(Hepatocyte growth factor, HGF)是一種多功能因子,生物活性由C-met蛋白介導(dǎo),能促進腫瘤細胞侵襲及新生血管生成。HGF主要由間質(zhì)細胞衍生的多功能細胞因子,通過自分泌或旁分泌的形式作用于數(shù)種上皮源性的細胞(如肝細胞、腎小管上皮細胞),誘導(dǎo)細胞有絲分裂、刺激細胞移動并且對抗細胞因子誘導(dǎo)的凋亡。HGF-c-Met信號傳導(dǎo)通路廣泛存在于各種細胞中,對多種組織器官的生長發(fā)育有重要的生理調(diào)節(jié)功能,HGF及其受體的研究,可能會為抗腫瘤轉(zhuǎn)移防治提供一個極有希望的靶點。 本研究擬收集在我院耳鼻咽喉科接受手術(shù)治療的病人新鮮組織標(biāo)本,慢性肥厚性鼻炎的下鼻甲粘膜12例(對照組),鼻內(nèi)翻性乳頭狀瘤40例,鼻腔鼻竇鱗癌14例。本實驗采用免疫組織化學(xué)方法檢測FSCN1和HGF在下鼻甲、鼻內(nèi)翻性乳頭狀瘤及鼻腔鱗癌組織中的表達,并對結(jié)果進行分析,探討FSCN1和HGF在N IP組織中的表達及其臨床意義。 材料與方法 1.收集2009年7月-2010年10月在我院耳鼻咽喉科接受手術(shù)治療的病人新鮮組織標(biāo)本,慢性肥厚性鼻炎的下鼻甲粘膜12例(對照組),鼻內(nèi)翻性乳頭狀瘤40例,鼻腔鼻竇鱗癌14例。所有病例術(shù)前均未行放療,化療等其他相關(guān)治療,臨床病歷資料完整,術(shù)后經(jīng)病理檢查診斷同術(shù)前。 2.采用免疫組化SP法分析FSCN和HGF在3種鼻病組織中的陽性表達。 3.采用SPSS13.0統(tǒng)計軟件包進行數(shù)據(jù)分析,X2檢驗及spearman等級相關(guān)分析。以P0.05有統(tǒng)計學(xué)意義。 結(jié)果 1.FSCN1蛋白主要表達于細胞漿,在下鼻甲組織中的陽性表達率為8.3%(1/12),在鼻內(nèi)翻性乳頭狀瘤組織中的陽性表達率為52.5%(21/40),在鼻腔鼻竇鱗癌組織中的陽性表達率為78.6%(11/14),三種鼻組織表達差異有統(tǒng)計學(xué)意義(P0.05)。 2.HGF主要表達于細胞漿,少數(shù)見于細胞核內(nèi),在下鼻甲組織中的陽性表達率為8.3%(1/12),在鼻內(nèi)翻性乳頭狀瘤組織中的陽性表達率為40.0%(16/40),在鼻腔鼻竇鱗癌組織中的陽性表達率為82.9%(12/14),三種鼻組織表達差異有統(tǒng)計學(xué)意義(P0.051)。 3.FSCN1/HGF在鼻內(nèi)翻性乳頭狀瘤組織中和鼻腔鼻竇鱗癌組織中的表達呈正相關(guān)(P0.05)。 結(jié)論 1. FSCN1和HGF在鼻內(nèi)翻性乳頭狀瘤組織中均高表達,提示FSCN1和HGF在鼻內(nèi)翻性乳頭狀瘤的發(fā)生和發(fā)展中起著重要的作用。 2. FSCN1和HGF均參與鼻內(nèi)翻性乳頭狀瘤的形成過程,但與鼻內(nèi)翻性乳頭狀瘤患者的病程、年齡及性別等因素?zé)o關(guān)。 3. FSCN1和HGF在鼻內(nèi)翻性乳頭狀瘤中的表達具有相關(guān)性,提示二者可能協(xié)同參與鼻內(nèi)翻性乳頭狀瘤的形成過程。 4. FSCN1和HGF在下鼻甲組織、鼻內(nèi)翻性乳頭狀瘤組織及鼻腔鼻竇鱗癌組織中的表達依次升高,提示二者在三種鼻病的轉(zhuǎn)化中起一定促進作用。 5. FSCN1和HGF的表達可反映鼻腔鼻竇腫瘤生物學(xué)行為的客觀指標(biāo),二者過度表達可能是鼻內(nèi)翻性乳頭狀瘤惡變?yōu)轺[癌的早期事件,通過檢測二者在三種鼻病的表達,為預(yù)防鼻腔鼻竇良惡性腫瘤的發(fā)生及診療提供新思路。
[Abstract]:Background and purpose
Nasal inverted papilloma (nasal inverted papilloma, NIP) is a more common benign tumor of the nasal sinus and nasal sinus, which accounts for the 0.4%-4.7% of the nasal sinus tumor. The male and female sex incidence is different, the male is significantly higher than the female, and the city is higher than the countryside. According to the reports, with the human diet, life and the surrounding area The effect of the environment, which has the clinical characteristics of invasive, high relapse and carcinogenesis, is complicated and causes many factors. The exact cause of the cause is not yet clear, and it may be caused by a variety of factors. With the study of the signal pathway conduction system in the field of molecular biology of tumor, the treatment of signal pathway conduction pathway is used. Cancer has become an emerging field of biotherapy.
Fascin protein belongs to a member of the structural protein family of the three leaf worm. The human Fascin-1 (FSCN1) gene is located on the chromosome 7p22. In normal tissues, FSCN1 is expressed in the interleaf tissue and the nervous system, and encodes a cytoskeleton protein of 55 kDa molecular mass. It can be combined with the actin with F- actin and located in the cytoplasm tension fibers and cells. The filamentous pseudo foot on the edge of the membrane folds, in the core actin bundle of the microspines, the thirty-ninth bit serine of the Fasein protein N terminal is the phosphorylation site of the protein kinase C (PKC). The phosphorylation of the site can regulate the binding activity of the Fascin protein with F-actin, and the formation of the filamentous and microspines on the surface of the cell membrane, suggesting that the Fascin protein may be migrated to the cell. Cell adhesion and intercellular information exchange play an important role in the formation and development of the tumor, promoting tumor invasion and metastasis.
Hepatocyte growth factor (HGF) is a multifunctional factor. Bioactivity is mediated by C-met protein, which promotes tumor cells invasion and neovascularization of the multifunctional cytokines derived mainly by interstitial cells and acts on several epithelial derived cells by autocrine or paracrine forms (such as liver cells). Renal tubular epithelial cells), inducing cell mitosis, stimulating cell migration and antagonism cell factor induced apoptosis.HGF-c-Met signal transduction pathway widely exists in various cells, and has important physiological regulating function for the growth and development of various tissues and organs. The study of HGF and its receptor may provide one for the prevention and control of anti tumor metastasis. A very promising target.
This study was to collect fresh tissue specimens of patients undergoing surgical treatment in our department of Otolaryngology, 12 cases of inferior turbinate mucosa of chronic hypertrophic rhinitis (control group), 40 cases of inverted papilloma of the nose, and 14 cases of nasal sinus squamous cell carcinoma. This experiment was conducted by immunohistochemistry to detect FSCN1 and HGF in the inferior turbinate, nasal inverted papilloma and nose. The expression of FSCN1 and HGF in N IP tissues and their clinical significance were analyzed.
Materials and methods
1. a collection of fresh tissue specimens, 12 cases of inferior turbinate mucosa of chronic hypertrophic rhinitis (control group), 40 cases of nasal inverted papilloma and 14 cases of nasal sinus squamous cell carcinoma were collected in the Department of Otolaryngology of our hospital in July 2009 -2010 years. All cases were not treated with radiotherapy, chemotherapy and other related treatments before operation. The clinical records were complete, After the operation, the pathological examination was performed before the operation.
2. immunohistochemical SP method was used to analyze the positive expression of FSCN and HGF in 3 kinds of rhinitis tissues.
3. using SPSS13.0 statistical package for data analysis, X2 test and Spearman rank correlation analysis. P0.05 was statistically significant.
Result
The positive expression rate of 1.FSCN1 protein was 8.3% (1/12) in the inferior turbinate tissue, and the positive expression rate in the nasal inverted papilloma was 52.5% (21/40). The positive expression rate in nasal sinus squamous cell carcinoma was 78.6% (11/14), and the difference of the three kinds of nasal tissues was statistically significant (P0.05).
The positive expression rate of 2.HGF was 8.3% (1/12) in the inferior turbinate tissue and 40% (16/40) in the nasal inverted papilloma tissue. The positive expression rate in nasal sinus squamous cell carcinoma was 82.9% (12 /14), and the three kinds of nasal tissue expression differences were statistically significant (P0.051).
3.FSCN1/HGF expression was positively correlated with sinonasal papilloma tissue and sinonasal squamous cell carcinoma (P0.05).
conclusion
1. FSCN1 and HGF were highly expressed in nasal inverted papilloma tissues, suggesting that FSCN1 and HGF play an important role in the occurrence and development of intranasal inverted papilloma.
2. FSCN1 and HGF were involved in the process of nasal inverted papilloma, but they were not related to the duration, age and sex of nasal inverted papilloma.
3. the expression of FSCN1 and HGF in nasal inverted papilloma is correlated, suggesting that the two may participate in the process of intranasal papilloma formation.
The expression of 4. FSCN1 and HGF in the inferior turbinate tissue, nasal inverted papilloma and nasal sinus squamous cell carcinoma increased in turn, suggesting that the two may play a role in the transformation of three kinds of nasal diseases.
The expression of 5. FSCN1 and HGF can reflect the objective index of biological behavior of nasal sinus tumor. The over expression of the two may be an early event of squamous carcinoma of inverted papilloma of the nose. By detecting the expression of two cases in three nasal diseases, a new way of prevention and treatment for the prevention and treatment of benign and malignant tumors of the nasal sinuses is provided.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R739.62

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本文編號：1971344