本文選題：喉腫瘤 + 反轉(zhuǎn)錄-聚合酶鏈反應(yīng)�。� 參考：《重慶醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的:研究MKK4基因在喉腫瘤中的表達(dá),探討它們的臨床意義及相互關(guān)系,為喉癌的發(fā)生發(fā)展、預(yù)后評估及尋求新的基因治療提供實(shí)驗(yàn)依據(jù)。 方法:運(yùn)用反轉(zhuǎn)錄-聚合酶鏈反應(yīng)(RT-PCR)和免疫組織化學(xué)方法檢測42例喉鱗癌、20例喉乳頭狀瘤及20例聲帶息肉組織中MKK4 mRNA和蛋白的表達(dá),分析它們在不同病變組織中的表達(dá)情況及與喉鱗癌臨床病理參數(shù)的關(guān)系。 結(jié)果: 1.MKK4 mRNA和蛋白表達(dá)的陽性率在喉鱗癌、喉乳頭狀瘤及聲帶息肉組織中逐漸降低,其差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 2.MKK4 mRNA和蛋白的表達(dá)隨喉鱗癌的浸潤深度增加而增加(P0.05),在淋巴結(jié)轉(zhuǎn)移組高于無淋巴結(jié)轉(zhuǎn)移組(P0.05),在臨床Ⅲ~Ⅳ期的表達(dá)高于臨床Ⅰ~Ⅱ的表達(dá)(P0.05)。 3.MKK4表達(dá)率與患者年齡、性別、腫瘤發(fā)生部位及組織學(xué)分級無相關(guān)性(P0.05)。 結(jié)論: 1.MKK4 mRNA和蛋白在喉鱗癌組織中的上調(diào)可能與喉鱗癌的發(fā)生及浸潤轉(zhuǎn)移有關(guān)。 2.MKK4可能作為一個潛在的判斷喉鱗癌發(fā)病與轉(zhuǎn)移的觀測指標(biāo)。
[Abstract]:Objective: to study the expression of MKK4 gene in laryngeal neoplasms, and to explore their clinical significance and relationship, so as to provide experimental evidence for the occurrence and development of laryngeal carcinoma, prognosis evaluation and search for new gene therapy. Methods: reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry were used to detect the expression of MKK4 mRNA and protein in 20 cases of laryngeal papilloma and 20 cases of vocal cord polyp. To analyze their expression in different pathological tissues and their relationship with the clinicopathologic parameters of laryngeal squamous cell carcinoma (LSCC). Results: The positive rates of 1.MKK4 mRNA and protein in laryngeal squamous cell carcinoma, laryngeal papilloma and vocal cord polyp decreased gradually, and the difference was statistically significant (P 0.05). The expression of 2.MKK4 mRNA and protein increased with the depth of invasion of laryngeal squamous cell carcinoma. The expression of 2.MKK4 mRNA and protein in lymph node metastasis group was higher than that in non-lymph node metastasis group, and the expression in clinical stage 鈪，

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