本文選題：IPO13 + P63�。� 參考：《中南大學(xué)》2011年碩士論文

【摘要】：目的：研究一個新近發(fā)現(xiàn)的細胞核質(zhì)雙向轉(zhuǎn)運受體蛋白importin13(IPO13)在人角結(jié)膜良、惡性腫瘤中的表達,并與角膜緣干細胞標記物p63作比較,探討兩者在人角結(jié)膜良、惡性腫瘤中的表達差異及意義。 方法：實驗分為三組：正常組6例,取自正常人眼球角膜緣處的結(jié)膜及角膜緣組織(來源于角膜移植術(shù)后的供體眼球)；角結(jié)膜乳頭狀瘤組(Conjunciva-cornea papilloma, CCP)6例、角結(jié)膜上皮內(nèi)上皮癌組(Conjunctiva-cornea intraepithelial neoplasia, CCIN)6例,腫塊標本均取自在湘雅醫(yī)院眼科住院部接受手術(shù)治療并經(jīng)病理檢查確診的患者。采用冰凍切片免疫組織化學(xué)染色法檢測IPO13、p63在人正常角結(jié)膜、CCP和CCIN三組角結(jié)膜組織中的差異性表達。 結(jié)果：1.IP013表達于人正常眼球結(jié)膜、角膜緣上皮基底細胞的細胞核內(nèi),表達于CCP、CCIN組織上皮基底細胞和基底上細胞的細胞核內(nèi),并且在正常組、CCP、CCIN組織中的表達逐步上調(diào),三組之間差異具有統(tǒng)計學(xué)意義(p0.05)。 2.p63表達于人正常眼球結(jié)膜、角膜緣、CCP組織上皮基底細胞和基底上細胞的細胞核內(nèi),在CCIN組織上皮全層的細胞核內(nèi)均有表達,并且在正常組、CCP、CCIN組織中的表達逐步上調(diào),三組之間差異具有統(tǒng)計學(xué)意義(p0.05)。 結(jié)論：1.IP013在標志角膜緣上皮增殖性病變中的低分化細胞較p63更具特異性。 2.與正常組相比,IP013與p63在CCP、CCIN組織中的表達逐步上調(diào),推測IPO13與p63可能在角結(jié)膜增殖性病變中起到正性調(diào)控作用。 3.IPO13與p63在良性與惡性角膜緣上皮增殖性病變中的表達差異明顯,提示IP013與p63可能在角膜緣上皮增殖性病變的異常增殖與分化中起到重要調(diào)控作用。
[Abstract]:Objective: to study the expression of a recently discovered nuclear cytoplasmic bidirectional transport receptor protein import in13 (IPO13) in human keratoconjunctiva benign and malignant tumors, and to compare it with corneal limbal stem cell marker p63, and to investigate the expression of the two proteins in human keratoconjunctiva. Expression difference and significance in malignant tumor. Methods: the experiment was divided into three groups: normal group (n = 6), conjunctival tissue and limbal tissue (from donor eyeball after keratoplasty), conjunctival papillomia (CCP)6), conjunctival papilloma group (n = 6), and conjunctival papillomia group (n = 6). Conjunctiva-cornea intraepithelial neoplasia, CCIN)6, were collected from patients who underwent surgical treatment and confirmed by pathological examination in the ophthalmic hospital of Xiangya Hospital. Immunohistochemical staining of frozen sections was used to detect the differential expression of IPO13 p63 in the conjunctiva of human normal conjunctiva and CCIN. Results 1. IP013 was expressed in the nucleus of the basal cells of corneal limbal epithelium, in the nucleus of epithelial basal cells of CCPCCIN, and in the nuclei of epithelial basal cells and suprabasal cells of CCPCCIN, and the expression of IP013 was gradually up-regulated in the normal group of conjunctival conjunctiva and corneal limbal epithelial basal cells. The difference between the three groups was statistically significant (P 0.05). The expression of 2.p63 was found in the nucleus of basal cells and suprabasal cells of normal conjunctiva, limbal keratoconjunctiva, and the whole layer of CCIN tissue, and increased gradually in the normal group. The difference between the three groups was statistically significant (P 0.05). Conclusion: 1. The low differentiation cells of IP013 in the marker of limbal epithelial proliferative lesions are more specific than p63. 2. Compared with the normal group, the expression of p63 and IP013 was gradually up-regulated in CCIN, suggesting that IPO13 and p63 might play a positive role in the regulation of conjunctival proliferative lesions. The expression of 3.IPO13 and p63 in benign and malignant corneal limbal epithelial proliferative lesions was significantly different, suggesting that IP013 and p63 might play an important role in the abnormal proliferation and differentiation of corneal limbal epithelial proliferative lesions.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R739.7

【共引文獻】

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本文編號：1957194