本文選題：青光眼 + 視網(wǎng)膜��； 參考：《山西醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的：探索口服米諾環(huán)素膠囊(minocycline)對兔慢性高眼壓模型視神經(jīng)損傷的保護作用,并探討其機制。 方法：取健康成年家兔48只,隨機分為A組(正常組)、B組(高眼壓組)和C組(高眼壓治療組),每組16只。B組和C組每只兔左眼為正常眼,右眼前房內(nèi)注射3g／L復(fù)方卡波姆溶液0.1mL,造慢性高眼壓模型。實驗以各組兔右眼為觀察對象。造模成功后,C組兔連續(xù)28d口服米諾環(huán)素膠囊,50mg／kg／24h。每周相同時間點測B組和C組兔眼壓,并記錄數(shù)據(jù)。第29d用耳緣靜脈注氣法處死各組動物,三組各隨機取8只兔,剝?nèi)∮已垡暰W(wǎng)膜,制成100g／L勻漿液�？捡R斯亮藍染色法(Commassie Blue Staining Kit)測出勻漿蛋白含量后以比色法測SOD、MDA、NO,并計算出各指標(biāo)含量。每組另外8個眼球進行常規(guī)固定、脫水、石蠟包埋切片,免疫組化法測caspase-3含量,陽性結(jié)果以胞漿或胞核內(nèi)出現(xiàn)棕黃色染色為準(zhǔn)。Image-Pro Plus5.0圖像分析軟件檢測每個標(biāo)本所測量的面積內(nèi),組織的平均灰度值(IOD)。SPSS13.0統(tǒng)計軟件分析處理每周相同時間點眼壓、超氧化物歧化酶(SOD)、丙二醛(MDA)、一氧化氮(NO)含量、caspase-3平均灰度值,并進行組間比較分析。 結(jié)果：B組和C組右眼比較,每周相同時間點眼壓測量結(jié)果無顯著統(tǒng)計學(xué)差異,均大于22mmHg。 caspase-3免疫組化檢測結(jié)果顯示,A組兔觀察不到棕黃色的陽性染色,視網(wǎng)膜caspase-3平均灰度值極低；B,C兩組可以觀察到相應(yīng)陽性染色顆粒。B組與A組比較,平均灰度值均較高,差異具有統(tǒng)計學(xué)意義；C組視網(wǎng)膜組織Caspase-3平均灰度值明顯低于B組,差異具有統(tǒng)計學(xué)意義；A組視網(wǎng)膜組織Caspase-3平均灰度值低于C組,差異具有統(tǒng)計學(xué)意義。 兔視網(wǎng)膜勻漿中SOD、NO、MDA表達測定結(jié)果顯示：與A組相比,B組SOD值明顯較低,NO、MDA較高,差異具有統(tǒng)計學(xué)意義；C組與B組相比,SOD值明顯較高；NO、MDA較低,差異具有統(tǒng)計學(xué)意義；C組SOD值明顯低于A組；NO、MDA明顯高于A組,差異具有統(tǒng)計學(xué)意義。 結(jié)論：兔眼前房內(nèi)注射復(fù)方卡波姆溶液,誘導(dǎo)出的慢性高眼壓模型觀察的時間內(nèi)眼壓可以維持在較高水平�？诜欢▌┝康拿字Z環(huán)素膠囊對慢性高眼壓下兔視網(wǎng)膜神經(jīng)節(jié)細(xì)胞的損傷有一定保護作用,其潛在作用機制可能是它所具有的抗凋亡、抗氧化的作用,但這種保護機制與眼壓無關(guān)。
[Abstract]:Aim: to investigate the protective effect of minocycline capsule on optic nerve injury in rabbits with chronic intraocular hypertension and its mechanism. Methods: Forty-eight healthy adult rabbits were randomly divided into two groups: group A (normal group) and group C (high intraocular pressure group), with 16 rabbits in each group and 16 rabbits in group C as normal eyes. The model of chronic high intraocular pressure was established by injecting 3g/L compound carbomer solution (0.1 mL) into right anterior chamber. The right eye of each group was observed. After the successful establishment of the model, the rabbits in group C took minocycline capsule 50 mg / kg / 24 h for 28 days. Intraocular pressure (IOP) of group B and group C were measured and recorded at the same time every week. On the 29th day, the animals in each group were killed by auricular vein injection. Eight rabbits were randomly selected from each group, and the right eye retina was stripped to make 100g/L homogenate. Coomassie brilliant blue staining method and Commassie Blue Staining Kit) were used to determine the content of homogenate protein, and then to measure the content of SDD MDAN by colorimetric method, and to calculate the contents of each index. The other 8 eyeballs in each group were routinely fixed, dehydrated, embedded in paraffin sections, and the content of caspase-3 was measured by immunohistochemical method. The positive results were detected by the brown-yellow staining in cytoplasm or nucleus. Image-Pro Plus5.0 image analysis software was used to detect the area of each specimen. The mean gray value of tissue was analyzed by IODX. SPSS 13.0 statistical software. IOP, SOD, MDA, no) content and caspase-3 mean gray value were analyzed at the same time point every week. Results there was no significant difference in intraocular pressure between group B and group C at the same time point every week, all of which were more than 22mm Hg. The results of caspase-3 immunohistochemical staining showed that the positive staining of brown was not observed in group A, and the average gray value of caspase-3 in group B was higher than that in group A, and that in group B was significantly higher than that in group A. The average gray value of Caspase-3 in group C was significantly lower than that in group B, and the average gray value of Caspase-3 in group A was lower than that in group C. the difference was statistically significant. The expression of SOD in rabbit retina homogenate was significantly lower than that in group A (P < 0.05). The difference was statistically significant between group C and group B (P < 0.05), and the level of SOD in group C was significantly higher than that in group B (P < 0.05). The SOD of group C was significantly lower than that of group A, and the difference was statistically significant. Conclusion: the intraocular pressure induced by the injection of compound carbomer solution into rabbit anterior chamber can be maintained at a high level during the observation period. A certain dose of minocycline capsule could protect the retinal ganglion cells from injury induced by chronic intraocular pressure, and its potential mechanism might be its anti-apoptosis and anti-oxidation effects. But this protective mechanism has nothing to do with intraocular pressure.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R77

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本文編號：1900779