本文選題：膽脂瘤 + 中耳��； 參考：《新疆醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的:探討凋亡抑制蛋白Livin及p73在中耳膽脂瘤上皮中發(fā)病機(jī)制的作用。方法:采用免疫組織化學(xué)技術(shù)SP法檢測(cè)30例中耳膽脂瘤上皮及16例外耳道正常皮膚組織中凋亡抑制蛋白Livin及p73的表達(dá),并結(jié)合患者臨床特征、中耳膽脂瘤對(duì)聽小骨骨質(zhì)破壞程度進(jìn)行分析。結(jié)果:凋亡抑制蛋白Livin表達(dá)于細(xì)胞核及部分細(xì)胞質(zhì),分布于膽脂瘤上皮全層,以基底層為主;p73表達(dá)于胞漿,分布于膽脂瘤上皮棘層和顆粒層;上述兩指標(biāo)在中耳膽脂瘤上皮中的陽(yáng)性表達(dá)率分別為76.7%、40%,凋亡抑制蛋白Livin在膽脂瘤上皮中的表達(dá)高于外耳道正常皮膚,差異有統(tǒng)計(jì)學(xué)意義(P0.05);p73在膽脂瘤上皮中的表達(dá)與外耳道正常皮膚之間無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);聽小骨骨質(zhì)破壞程度、性別、民族、膽脂瘤侵犯范圍與上述指標(biāo)的表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05);膽脂瘤上皮中凋亡抑制蛋白Livin的表達(dá)與p73的表達(dá)呈正相關(guān)(r=0.411,P0.05)。結(jié)論:凋亡抑制蛋白Livin在中耳膽脂瘤的異常表達(dá)可能在膽脂瘤的發(fā)生、發(fā)展過(guò)程中起相應(yīng)作用,可能參與膽脂瘤上皮的凋亡調(diào)控過(guò)程,而p73在中耳膽脂瘤的發(fā)生和發(fā)展中的作用尚難確定。膽脂瘤上皮的凋亡與增殖與聽小骨破壞程度尚無(wú)直接關(guān)系。
[Abstract]:Objective: to investigate the role of Livin and p73 in the pathogenesis of middle ear cholesteatoma. Methods: immunohistochemical SP method was used to detect the expression of Livin and p73 in 30 cases of middle ear cholesteatoma and 16 cases of normal skin of external auditory canal. Middle ear cholesteatoma was used to analyze the degree of destruction of osseous auditory bone. Results: Livin was expressed in the nucleus and some cytoplasm, distributed in the whole layer of cholesteatoma epithelium, mainly in the cytoplasm of the basal layer, in the spinous layer and granular layer of the epithelium of cholesteatoma. The positive expression rates of these two markers in the epithelium of middle ear cholesteatoma were 76. 7% and 40%, respectively. The expression of apoptosis inhibitor protein Livin in the epithelium of cholesteatoma was higher than that in normal skin of external auditory canal. There was no significant difference between the expression of P0.05P73 in the epithelium of cholesteatoma and the normal skin of external auditory canal. There was no significant difference between the extent of cholesteatoma invasion and the expression of the above indexes. The expression of apoptosis inhibitor protein (Livin) in cholesteatoma epithelium was positively correlated with the expression of p73. Conclusion: the abnormal expression of apoptosis inhibitor protein Livin in middle ear cholesteatoma may play a corresponding role in the occurrence and development of cholesteatoma, and may participate in the process of apoptosis regulation of cholesteatoma epithelium. The role of p73 in the development and development of middle ear cholesteatoma is uncertain. There is no direct relationship between apoptosis and proliferation of cholesteatoma epithelium and the degree of destruction of auditory bone.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R764

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