本文選題：角膜 + 新生血管　； 參考：《南昌大學(xué)》2011年碩士論文

【摘要】：目的： 通過(guò)研究阿瓦斯汀不同的給藥途徑分別對(duì)兔角膜新生血管及超微結(jié)構(gòu)的影響,探索阿瓦斯汀治療角膜新生血管較安全持久有效的給藥途徑,為臨床應(yīng)用阿瓦斯汀治療角膜新生血管性疾病提供實(shí)驗(yàn)依據(jù)。 方法： 選用健康新西蘭大白兔55只,兔齡2.5-3個(gè)月,雌雄不限。隨機(jī)選50只兔左眼用1mol/L氫氧化鈉溶液燒傷,建立堿燒傷角膜新生血管動(dòng)物模型,造模成功后選取48只隨機(jī)分為A點(diǎn)眼液組12只(5mg/ml,3次/日)、B結(jié)膜下注射組12只(25mg/ml,1次/周),C角膜基質(zhì)注射組12只(25mg/ml,1次/周),D模型對(duì)照組12只(不用藥),選取未造模2只兔為空白對(duì)照,于堿燒傷術(shù)后1天、4天、給藥后一周、二周、四周觀察造模兔眼結(jié)膜充血、角膜渾濁、新生血管生長(zhǎng)情況及角膜組織的完整性,并進(jìn)行眼前節(jié)照相,并測(cè)量各試驗(yàn)兔眼角膜新生血管的長(zhǎng)度及分布鐘點(diǎn)位數(shù)以計(jì)算角膜新生血管面積,與給藥后一周、二周、四周三個(gè)時(shí)間點(diǎn)后用空氣栓塞法每組各處死4只試驗(yàn)用兔,處死后即刻抽取試驗(yàn)眼房水約0.1m1用于檢測(cè)房水中的VEGF蛋白的含量,并取新生血管生長(zhǎng)最旺盛的角膜組織分別固定,待做電鏡、免疫組化(角膜組織中CD31)等檢測(cè)。 結(jié)果： 1、在堿燒傷后各觀察時(shí)間點(diǎn)房水中VEGF的濃度均高于正常兔眼水平,堿燒傷后11天內(nèi)房水中VEGF蛋白含量增加迅速,約18天左右時(shí)房水中VEGF蛋白含量達(dá)到最高水平,之后有所下降,到32天時(shí)達(dá)到一個(gè)較穩(wěn)定的水平,不同處理方法之間相比較在三個(gè)時(shí)間點(diǎn)差別均有統(tǒng)計(jì)學(xué)意義。 2、堿燒傷角膜新生血管在堿燒傷后12天內(nèi)生長(zhǎng)迅速,14天時(shí)有所消退,之后新生血管趨于相對(duì)穩(wěn)定狀態(tài),不同處理方法之間相比較在三個(gè)時(shí)間點(diǎn)差別均有統(tǒng)計(jì)學(xué)意義。 3、免疫組化染色結(jié)果示：CD31在正常兔眼角膜組織中未有表達(dá),各治療組陽(yáng)性細(xì)胞數(shù)明顯少于模型對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義。 4、電鏡檢查結(jié)果：ABD組角膜超微結(jié)構(gòu)在一周、二周、四周時(shí)除堿燒傷損傷外無(wú)明顯其它改變,在給藥后第二周、四周時(shí)C組角膜超微結(jié)構(gòu)損傷要重于ABD組。 結(jié)論： 1、兔眼房水中VEGF蛋白含量在堿燒傷后11天內(nèi)增長(zhǎng)較快,至18天左右時(shí)達(dá)到高峰水平,之后VEGF有所下降,最后趨于較穩(wěn)定水平。 2、點(diǎn)眼液(5mg/ml,3次/日),結(jié)膜下注射(25mg/ml,1次/周),角膜基質(zhì)注射(25mg/ml,1次/周)三種給藥途徑都對(duì)堿燒傷角膜新生血管增殖期動(dòng)物模型有較好的抑制作用,同時(shí)也能減輕角膜的混濁。 3、點(diǎn)眼液與結(jié)膜下注射給藥途徑簡(jiǎn)單安全、效果相對(duì)穩(wěn)定,在觀察期內(nèi)未見(jiàn)對(duì)角膜超微結(jié)構(gòu)的產(chǎn)生明顯影響。 4、角膜基質(zhì)注射給藥途徑雖短期效果顯著,但在觀察期內(nèi)對(duì)角膜超微結(jié)構(gòu)產(chǎn)生較明顯的影響。
[Abstract]:Objective: By studying the effects of different administration routes of Awa statin on corneal neovascularization and ultrastructure in rabbits, we explored the safe and effective way of administration of Awa statin in the treatment of corneal neovascularization. To provide experimental evidence for clinical treatment of corneal neovascular diseases with Awa statin. Methods: A total of 55 healthy New Zealand white rabbits (2.5-3 months old) were selected. A corneal neovascularization model was established in 50 rabbits with 1mol/L sodium hydroxide solution burn. 48 rats were randomly divided into A point group (n = 12) with 5 mg / ml / d subconjunctival injection group (n = 12) with 25 mg / ml once a week / week injection group (n = 12) and control group (n = 12). Rabbits were used as blank control. The conjunctival congestion, corneal opacification, neovascularization and corneal tissue integrity were observed one week, two weeks after alkali burn and one week, two weeks after administration. The corneal neovascularization area was calculated by measuring the length of corneal neovascularization and the number of hours of corneal neovascularization in each group. Four experimental rabbits were killed in each group by air embolization one week, two weeks and four weeks after administration of the drug. About 0.1m1 was extracted immediately after death to detect the content of VEGF protein in aqueous humor, and the corneal tissues with the most vigorous neovascularization were fixed respectively, and were examined by electron microscope and immunohistochemistry (CD31 in corneal tissue). Results: 1. The concentration of VEGF in aqueous humor was higher than that in normal rabbit eyes at all time points after alkali burn. The content of VEGF protein in aqueous humor increased rapidly within 11 days after alkali burn, and the content of VEGF protein in aqueous humor reached the highest level at about 18 days, and then decreased. At 32 days, a relatively stable level was reached, and the differences among different treatment methods were statistically significant at three time points. 2. Corneal neovascularization after alkali burn subsided rapidly within 12 days after alkali burn, then the neovascularization tended to be relatively stable. There were significant differences among different treatment methods at three time points. 3. The results of immunohistochemical staining showed that there was no expression of CD31 in normal rabbit cornea, and the number of positive cells in each treatment group was significantly lower than that in model control group (P < 0.05). (4) the ultrastructure of cornea in group C was more serious than that in group ABD at the second week after administration of the drug, and there was no obvious change of corneal ultrastructure except alkali burn injury at one week, two weeks and four weeks after administration of the drug. Conclusion: 1. The content of VEGF protein in aqueous humor of rabbit eyes increased rapidly within 11 days after alkali burn, reached the peak level at about 18 days, then VEGF decreased and finally became stable. 2, 5 mg / ml / day, 5 mg / ml / day, 25 mg / ml / week, and 25 mg / ml / week respectively) all of the three ways have a good inhibitory effect on corneal neovascularization model of alkali burn, and can also reduce corneal opacity. 3. The route of injection of point eye liquid and subconjunctival injection was simple and safe, the effect was relatively stable, and there was no obvious effect on the ultrastructure of cornea during the observation period. 4. The short-term effect of corneal stroma injection was remarkable, but it had obvious effect on corneal ultrastructure during the observation period.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R772.2

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