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  本文選題：激光拉曼光譜 + 鼻咽癌�。� 參考：《福建醫(yī)科大學(xué)》2010年碩士論文

【摘要】： 目的研究激光拉曼光譜技術(shù)對于鼻咽癌血清早期篩查診斷的應(yīng)用價值。 方法從2009年5月到2009年12月,對我院收治的29例經(jīng)病理證實為鼻咽癌的初診患者和17例健康志愿者抽取外周靜脈血2-4ml于血常規(guī)管中。所有的血液樣品經(jīng)離心后取上清液獲得血清樣品。采用inVia型共聚焦顯微拉曼光譜儀進行測量,每個樣品測5個點。將同一樣品所測量的5條光譜進行平均以代表一個樣品的血清拉曼光譜信號。最后將鼻咽癌患者及健康志愿者兩組拉曼光譜信號數(shù)據(jù)、鼻咽癌血清EBV-DNA陰性及陽性兩亞組的拉曼光譜數(shù)據(jù)采用成組t檢驗,主成分分析,判別分析等進行統(tǒng)計學(xué)分析處理。 結(jié)果鼻咽癌患者和健康志愿者兩組血清平均拉曼光譜的譜峰基本相似,但多數(shù)譜峰位置的頻移差別有統(tǒng)計學(xué)意義。常見譜峰I1445、I1651的強度及I1445%qI1651比值的差別無統(tǒng)計學(xué)意義(P值分別為:0.08、0.51、0.41)。進一步采用主成分降維處理后予判別分析,兩組血清的回顧性判別敏感性為100%,特異性為100%,誤判概率為0%,而交叉驗證判別敏感性為100%,特異性為93.1%(27/29),誤判概率為4.3%(2/46)。鼻咽癌血清EBV-DNA陰性及陽性兩亞組血清回顧性判別敏感性為91.7%,特異性為90.9%,誤判概率為8.7%(2/23),而交叉驗證判別敏感性41.7%(5/12),特異性45.5%(5/11),誤判概率為56.5%(13/23)。 結(jié)論激光拉曼光譜技術(shù)可很好的區(qū)分鼻咽癌血清及正常人血清,尚無法區(qū)分鼻咽癌患者EBV-DNA陰性與EBV-DNA陽性的血清。雖然我們的研究結(jié)果令人鼓舞,但仍需要進一步的大宗病例進行驗證。
[Abstract]:Objective to study the value of laser Raman spectroscopy in early diagnosis of nasopharyngeal carcinoma (NPC). Methods from May 2009 to December 2009, 29 patients with nasopharyngeal carcinoma (NPC) and 17 healthy volunteers were treated in our hospital. The peripheral venous blood 2-4ml was extracted from the blood routine tube. All blood samples were taken from supernatant after centrifugation to obtain serum samples. InVia confocal microscopy Raman spectrometer was used to measure 5 points for each sample. The 5 spectra measured by the same sample were averaged to represent the serum Raman spectrum signal of one sample. At last, the Raman signal data of NPC patients and healthy volunteers, and the Raman spectrum data of serum EBV-DNA negative and positive subgroups of NPC were analyzed statistically by group t test, principal component analysis and discriminant analysis. Results the average Raman spectra of patients with nasopharyngeal carcinoma and healthy volunteers were similar, but the frequency shift of most peaks was statistically significant. There was no significant difference in the intensity and I1445%qI1651 ratio of the common spectral peak I1445 / I1651 (P = 0.08 / 0.51 / 0.41). The retrospective discriminant sensitivity of the two groups was 100, the specificity was 100, the probability of misjudgment was 0, the sensitivity of cross-validation was 100, the specificity was 93.1% 27 / 29, the probability of misjudgment was 4.33%. The sensitivity of retrospective discriminant was 91.7, the specificity was 90.9, the probability of misjudgment was 8.7 / 23 / 23, the sensitivity of cross-validation was 41.7 / 12, the specificity was 45.5 / 11, and the probability of misjudgment was 56.513 / 230.The results showed that the sensitivity of serum EBV-DNA negative and positive was 91.7%, the specificity was 90.9%, the probability of misjudgment was 8.7% / 23%, and the sensitivity of cross-validation was 41.7% / 12%, and the specificity was 45.5% / 11%. Conclusion Laser Raman spectroscopy can distinguish NPC serum from normal serum, but can not distinguish EBV-DNA negative serum from EBV-DNA positive serum. While our findings are encouraging, further large numbers of cases are needed.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.63

【參考文獻】

相關(guān)期刊論文 前10條

1 佟倜,葉松,安利民,楊秀蘭,孫迎春,金新,王錫錄;鱗癌及其癌旁組織的共聚焦激光拉曼光譜分析[J];吉林大學(xué)學(xué)報(醫(yī)學(xué)版);2004年05期

2 柯惟中,余多慰,莊振武;UVA和UVB區(qū)段紫外線照射對DNA影響的拉曼光譜分析[J];光散射學(xué)報;1999年04期

3 李維紅,徐怡莊,翁詩甫,彭卿,徐智,吳奇光,孫開華,周孝思,吳瑾光;腫瘤和正常組織的FT-Raman與FT-IR研究[J];光散射學(xué)報;1998年Z1期

4 郭萍,易光輝,熊平,袁亞莉,謝琪,陳春先;癌癥患者血清的激光拉曼光譜[J];光譜學(xué)與光譜分析;2000年06期

5 閆循領(lǐng),董瑞新,王秋國,陳雙峰,張宗旺,張學(xué)俊,張雷;乳腺癌病人單個細(xì)胞的Raman光譜[J];光譜學(xué)與光譜分析;2005年01期

6 駱智訓(xùn);方炎;;表面增強拉曼散射光譜的應(yīng)用進展[J];光譜學(xué)與光譜分析;2006年02期

7 李蓉;周光明;彭紅軍;鄧永利;;甲狀腺癌組織的傅里葉變換拉曼光譜研究[J];光譜學(xué)與光譜分析;2006年10期

8 姚輝璐;朱淼;王桂文;彭立新;何碧娟;何敏;黎永青;;單個鼻咽癌細(xì)胞的拉曼光譜分析的研究[J];光譜學(xué)與光譜分析;2007年09期

9 劉燕楠;鄒祖全;劉燕青;徐曉軒;俞鋼;張存洲;;肺正常組織與癌變組織的表面增強拉曼光譜[J];光譜學(xué)與光譜分析;2007年10期

10 姚淑霞,趙元黎,張錄,申培紅,葛向紅;乳腺組織切片的拉曼光譜的研究[J];河南預(yù)防醫(yī)學(xué)雜志;2004年03期

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