本文選題：間歇低氧 + N-乙酰半胱氨酸　； 參考：《蘇州大學(xué)》2011年碩士論文

【摘要】：目的觀察慢性間歇低氧((chronic intermittent hypoxia,CIH)小鼠血清氧化應(yīng)激指標(biāo)的變化情況,并初步探討N-乙酰半胱氨酸(N-acetylcystein,NAC)的治療作用。 方法將50只小鼠隨機(jī)分為正常對(duì)照組(unhandled control group, UC) 20只,CIH組30只,分別進(jìn)行間歇低氧3天、1周、2周、3周、4周;另設(shè)CIH 4周+NAC組6只。分別采用硫代巴比妥酸法、羥胺法、化學(xué)比色法檢測(cè)各組小鼠血清丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)、和谷胱甘肽過(guò)氧化物(glutathione peroxidase,GSH-PX)表達(dá)水平,觀察隨著間歇低氧造模時(shí)間延長(zhǎng),各指標(biāo)的變化情況,以及NAC的治療作用。 結(jié)果(1)在間歇低氧造模第3天時(shí),CIH組小鼠血清MDA水平較對(duì)照組有所升高,較UC組比較差異有統(tǒng)計(jì)學(xué)意義,(CIH 8.98±0.50 nmol/mL UC 7.82±0.49 nmol/mL,p0.05)。隨著間歇低氧時(shí)間的延長(zhǎng),CIH組小鼠血清MDA水平呈逐漸升高趨勢(shì),各造模時(shí)間段兩組之間比較,差異均有統(tǒng)計(jì)學(xué)意義(p0.05或p0.01)。另外,在間歇低氧造模3天時(shí),CIH組小鼠血清SOD、GSH-PX水平較對(duì)照組有所下降,但差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05);自低氧第1周始,CIH組小鼠血清SOD、GSH-PX分別為83.30±7.32U/mL、211.5±13.39 U/mL,較UC組95.14±8.4 U/mL、233.84.±15.32 U/mL比較差異有統(tǒng)計(jì)學(xué)意義(p0.05)。隨著間歇低氧時(shí)間的延長(zhǎng),CIH組小鼠血清SOD、GSH-PX水平呈逐漸下降趨勢(shì),各造模時(shí)間段兩組之間比較,差異均有統(tǒng)計(jì)學(xué)意義(p0.05或p0.01)。 (2)間歇低氧4周組+NAC組與單獨(dú)間歇低氧4周組相比,SOD,GSH-PX升高,MDA降低,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。但MDA仍高于UC組,SOD、GSH-PX仍低于UC組,差異有統(tǒng)計(jì)學(xué)意義(P0.05或P0.01)。 結(jié)論CIH可導(dǎo)致機(jī)體氧化應(yīng)激水平增加,且隨著低氧時(shí)間延長(zhǎng),機(jī)體氧化抗氧化失衡越嚴(yán)重。NAC治療能夠改善CIH導(dǎo)致的氧化應(yīng)激反應(yīng)。
[Abstract]:Objective to observe the changes of serum oxidative stress indexes in chronic intermittent hypoxia intermittent mice and to explore the therapeutic effect of N-acetylcystein Nacetylcysteine (N-acetylcystein). Methods Fifty mice were randomly divided into control group (n = 30) and control group (n = 20). They were treated with intermittent hypoxia for 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks, and CIH for 4 weeks, NAC group, 6 rats, respectively. The serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and glutathione peroxidase (GSH-PX) were detected by thiobarbituric acid method, hydroxylamine method and chemical colorimetry respectively. The changes of each index and the therapeutic effect of NAC. Results 1) on the 3rd day after intermittent hypoxia, the serum MDA level of mice in the control group was higher than that in the control group, which was significantly higher than that in the UC group (8.98 鹵0.50 nmol/mL UC 7.82 鹵0.49 nmol / mL). With the prolongation of intermittent hypoxic time, the serum MDA level of mice increased gradually, and there were significant differences between the two groups in each model time period (p0. 05 or p0. 01). In addition, the serum level of GSH-PX in the CIH group was lower than that in the control group after 3 days of intermittent hypoxia, but the difference was not significant (p 0.05), and the serum GSH-PX in the CIH group was 83.30 鹵7.32 U / mL 211.5 鹵13.39 U / mLrespectively since the first week of hypoxia, which was significantly higher than that in the UC group (95.14 鹵8.4 U / mL / L 233.84 鹵15.32 U/mL). The level of GSH-PX in serum of CIH group decreased gradually with the prolongation of intermittent hypoxia time, and there were significant differences between the two groups in each model time period (P 0.05 or p 0.01). (2) compared with intermittent hypoxia for 4 weeks, the GSH-PX level in NAC group was significantly lower than that in intermittent hypoxia group for 4 weeks, and the difference was statistically significant (P 0.05). However, MDA was still higher in UC group than in UC group, and GSH-PX was still lower than that in UC group (P 0.05 or P 0.01). Conclusion CIH can increase the level of oxidative stress, and with the prolongation of hypoxic time, the more serious the imbalance of oxidative oxidation. NAC can improve the oxidative stress response induced by CIH.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R766.43

【引證文獻(xiàn)】

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1 張婷婷;血清8-OHdG、MnSOD在阻塞性睡眠呼吸暫停低通氣綜合征合并2型糖尿病患者中的變化及意義[D];山西醫(yī)科大學(xué);2012年

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本文編號(hào)：1802545