本文選題：視神經 + 閃光視覺誘發(fā)電位��； 參考：《中國醫(yī)科大學》2010年碩士論文

【摘要】： 目的 觀察低能量激光照射對大鼠視神經損傷后傷側眼閃光視覺誘發(fā)電位(F-VEP)潛伏時、視網(wǎng)膜神經節(jié)細胞(retinal ganglion cells,RGCs)數(shù)量、睫狀神經營養(yǎng)因子(ciliary neurotrophic factor,CNTF)表達水平的影響。探討低能量激光照射對大鼠視神經不完全損傷的影響及作用機制。 方法 隨機抽取5只大鼠作為正常組,剩余的45只大鼠分為：傷后3d、7d、14d 3個時間點,每個時間點各15只大鼠再隨機分為治療組10只、對照組5只。除正常組外,全部大鼠均手術,造成視神經不完全損傷模型后,治療組給予低能量激光照射治療,對照組不給于處理因素。 在損傷后1d、3d、5d、7d、10d、14d動態(tài)觀測并比較對照組、治療組大鼠傷側眼F-VEP潛伏時變化。在相應的時間分別處死各時間點15只大鼠,取傷側眼球固定、包埋,通過HE及免疫組化染色方法,測定對照組、治療組及正常組RGCs數(shù)量、CNTF表達水平,并進行統(tǒng)計分析。 結果 視神經損傷后F-VEP潛伏時較正常眼明顯延長。治療組與對照組相比,損傷后3d、5d、7d和10d,潛伏時縮短,兩組差異具有統(tǒng)計學意義(P0.05),而傷后1d和14d,F-VEP潛伏時差異無統(tǒng)計學意義(P0.05)。 視神經不完全損傷后視網(wǎng)膜節(jié)細胞(RGCs)存活數(shù)量逐漸減少,損傷3天內減少速度慢,細胞數(shù)減少不明顯,7天后RGCs減少速度加快,細胞數(shù)大量減少。在損傷后7d,治療組RGCs存活數(shù)量較對照組增多,兩組差異具有統(tǒng)計學意義(P0.05),而損傷后3d和14d,兩組RGCs數(shù)差異無統(tǒng)計學意義(P0.05)。 視神經不全損傷后,各時間點(3d、7d、14d)對照組CNTF表達IOD值均較正常組增加,兩組各時間點差異均具有統(tǒng)計學意義(p0.05)。治療組與對照組相比,在損傷后7天,兩組視網(wǎng)膜CNTF表達的差異具有統(tǒng)計學意義(P0.05),而損傷后3天和14天,兩組視網(wǎng)膜CNTF表達的差異無統(tǒng)計學意義(P0.05)。 結論 低能量激光照射治療可縮短視神經不全損傷所致F-VEP潛伏時延長,緩解視神經損傷造成的視覺傳導障礙；可增加視神經不全損傷后1周視網(wǎng)膜節(jié)細胞存活數(shù)量,減輕RGCs丟失的程度；可明顯增加損傷后1周大鼠視網(wǎng)膜CNTF蛋白表達,提高視神經再生修復因子CNTF表達的數(shù)量。
[Abstract]:Purpose To observe the effects of low energy laser irradiation on the number of retinal ganglion cells and the expression of ciliary neurotrophic factor (CNTF) in the injured eyes of rats with optic nerve injury. To investigate the effect and mechanism of low energy laser irradiation on incomplete optic nerve injury in rats. Method Five rats were randomly selected as normal group. The remaining 45 rats were divided into 3 time points: 3 days after injury, 15 rats at each time point were randomly divided into treatment group (n = 10) and control group (n = 5). All the rats were operated on except the normal group. After the model of incomplete optic nerve injury was established, the treatment group was treated with low energy laser irradiation, while the control group was not given the factors of treatment. The dynamic changes of F-VEP in the injured eyes of rats in the treatment group were observed and compared with those in the control group at 1 day, 3 days, 5 days, 7 days, 10 days and 14 days after injury, and the changes of latent time of F-VEP in the injured eyes of the treatment group were compared. At the corresponding time, 15 rats were killed at each time point, and the injured side eyeball was fixed and embedded. The expression of RGCs in control group, treatment group and normal group was determined by HE and immunohistochemical staining, and the expression of RGCs was analyzed statistically. Result F-VEP latency after optic nerve injury was significantly longer than that in normal eyes. Compared with the control group, the latency time of the treatment group was shorter than that of the control group at 3 and 10 days after injury. The difference between the two groups was statistically significant (P 0.05), but there was no significant difference between the two groups in the F-VEP latency at 1 and 14 days after injury (P 0.05). The survival rate of retinal ganglion cells (RGCs) decreased gradually after incomplete optic nerve injury, and the reduction rate of RGCs was slow in 3 days after injury. After 7 days, the decrease rate of RGCs increased and the number of cells decreased significantly. On the 7th day after injury, the number of RGCs survival in the treatment group was higher than that in the control group. The difference between the two groups was statistically significant (P 0.05), but there was no significant difference in the RGCs number between the two groups on the 3rd day and the 14th day after injury (P 0.05). The IOD expression of CNTF in the control group was significantly higher than that in the control group, and the difference between the two groups at each time point was statistically significant (P 0.05). Compared with the control group, the expression of CNTF in the retina of the treatment group was significantly different from that in the control group on the 7th day after injury, but there was no significant difference in the expression of CNTF between the two groups on the 3rd and 14th day after injury. Conclusion Low energy laser irradiation can shorten the latency of F-VEP induced by incomplete optic nerve injury, alleviate the visual conduction disorder caused by optic nerve injury, and increase the number of retinal ganglion cells survival after 1 week of optic nerve incomplete injury. Reducing the loss of RGCs significantly increased the expression of CNTF protein in the retina and increased the expression of CNTF in the optic nerve regeneration repair factor 1 week after injury.
【學位授予單位】：中國醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R454.2;R779.1

【參考文獻】

相關期刊論文 前4條

1 劉婉瑩,張卯年,陳兵,鞠躬,盧華;SD大鼠視神經不同程度損傷的閃光視覺誘發(fā)電位動態(tài)監(jiān)測[J];解放軍醫(yī)學雜志;2004年05期

2 鄒倩,葉劍,朱佩芳,王正國,李澤桂;大鼠視神經損傷后視網(wǎng)膜CNTF及CNTFRα mRNA的表達[J];眼科研究;2004年01期

3 胡愛蓮,孫葆忱,董東生,劉守彬;正常及視神經損傷大鼠閃光視覺誘發(fā)電位[J];眼科;2000年01期

4 胡愛蓮,孫葆忱,李遼青,孫憲麗,劉守彬,董冬生;大鼠視神經夾挫傷視網(wǎng)膜視神經病理學動態(tài)觀察及功能檢測[J];眼科;2003年06期

，

本文編號：1802187