本文選題：中波紫外線 + 晶狀體上皮細(xì)胞�。� 參考：《瀘州醫(yī)學(xué)院》2010年碩士論文

【摘要】： 目的：探討用中波紫外線照射SD大鼠建立紫外線誘導(dǎo)的動(dòng)物白內(nèi)障模型及對(duì)比三種晶狀體圖像采集的效果,從而為進(jìn)一步研究白內(nèi)障的形成,混濁程度的評(píng)定及白內(nèi)障的防治提供相關(guān)實(shí)驗(yàn)依據(jù)。方法：1.選用3月齡的一級(jí)大白鼠20只,隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組,實(shí)驗(yàn)組大鼠置于20×40×20cm3的籠中,籠子上方安置中波紫外線光源,光源距離大鼠約40cm,給予每天2小時(shí)中波紫外線照射；對(duì)照組不給于任何干預(yù)處理。選擇不同照射時(shí)間段大鼠(0天、5天、20天、40天)摘取眼球去除角膜后在眼前節(jié)照相系統(tǒng)下采集晶狀體混濁程度及晶狀體核的顏色變化。然后環(huán)形剪下晶狀體前囊膜,細(xì)胞面向上鋪于玻片上,TUNEL法檢測(cè)晶狀體上皮細(xì)胞凋亡情況。觀察晶狀體上皮細(xì)胞的形態(tài)變化。2.另選4只大白鼠,用中波紫外線照射40天,采集眼球后對(duì)8只眼球同時(shí)選用三種方法采集白內(nèi)障晶狀體圖像,并對(duì)比分析三種方法所獲圖像的效果。結(jié)果：1.眼前節(jié)照相系統(tǒng)觀察晶狀體：實(shí)驗(yàn)組結(jié)果顯示經(jīng)中波紫外線照射活體SD大鼠每天2小時(shí),在第5天時(shí)晶狀體保持透明、無(wú)明顯混濁,隨著照射時(shí)間的延長(zhǎng),在20天時(shí)出現(xiàn)皮質(zhì)輕度混濁、多集中在“Y”字縫附近,呈現(xiàn)前部片狀、羽毛狀朝向赤道部的淺層皮質(zhì)混濁,顏色呈白色,未見(jiàn)晶狀體核的透明性及顏色改變。在40天時(shí)前囊下皮質(zhì)混濁程度更加明顯,前部淺皮質(zhì)層及深皮質(zhì)層均可觀察到白色混濁,范圍可以擴(kuò)大到近赤道部；在手術(shù)摘取前囊膜時(shí)可見(jiàn)多數(shù)大鼠淺層混濁的皮質(zhì)與前囊膜粘連較正常組緊密。40天照射組的晶狀體核未發(fā)現(xiàn)有明顯顏色變化。對(duì)照組未發(fā)現(xiàn)晶狀體皮質(zhì)及晶狀體核有明顯混濁和顏色的變化,仍保持其透明性。2. TUNEL細(xì)胞凋亡檢測(cè)：DNA缺口末端原位檢測(cè)部分大鼠晶狀體上皮細(xì)胞發(fā)現(xiàn)經(jīng)紫外線照射活體大鼠5天時(shí)即可發(fā)現(xiàn)晶狀體前囊膜上皮細(xì)胞出現(xiàn)凋亡,凋亡的細(xì)胞呈棕黃色。3.細(xì)胞形態(tài)變化：5天組的細(xì)胞形態(tài)、大小與對(duì)照組相比無(wú)明顯改變。40天組部分晶狀體上皮細(xì)胞出現(xiàn)老化現(xiàn)象,可見(jiàn)大量的晶狀體纖維與晶狀體上皮細(xì)胞相連。4.對(duì)同一只白內(nèi)障晶狀體選用不同的三種晶狀體采集方法獲得晶狀體圖像,相比而言本實(shí)驗(yàn)設(shè)計(jì)的晶狀體托板聯(lián)合眼前節(jié)照相系統(tǒng)法采集的圖像較其它兩種方法獲得的圖像更清晰,結(jié)果穩(wěn)定。結(jié)論：1.本實(shí)驗(yàn)方法成功實(shí)現(xiàn)了紫外線輻射性白內(nèi)障大鼠模型的建立。2.中波紫外線是導(dǎo)致白內(nèi)障發(fā)生的原因之一。3.晶狀體混濁程度與中波紫外線照射的時(shí)間長(zhǎng)短呈正相關(guān)。4.中波紫外線照射活體SD大鼠眼可以造成晶狀體前囊膜上皮細(xì)胞凋亡。5.三種白內(nèi)障晶狀體圖像采集方法各有優(yōu)缺點(diǎn),本實(shí)驗(yàn)采用黑色晶狀體托板聯(lián)合眼前節(jié)照相系統(tǒng)可以獲得理想的晶狀體圖像,與其它兩種方法相比較具有明顯優(yōu)勢(shì)。
[Abstract]:Objective: to investigate the effects of ultraviolet irradiation on the formation of cataract in SD rats, and to compare the effects of three kinds of lens image collection in order to further study the formation of cataract. The evaluation of turbidity degree and the prevention and treatment of cataract provide relevant experimental basis. Method 1: 1. Twenty 3-month-old first-grade rats were randomly divided into experimental group and control group. Rats in the experimental group were placed in a cage of 20 脳 40 脳 20cm3, and ultraviolet B light source was placed above the cage. The distance from the light source to the rats was about 40 cm, and the rats were irradiated with ultraviolet B for 2 hours a day. The control group was not given any intervention. The degree of lens opacity and the color change of lens nucleus were collected under the anterior segment radiography system after the eyeball was removed from the cornea. Then the lens anterior capsule membrane was cut in a circular way, and the apoptosis of lens epithelial cells was detected by Tunel method. To observe the morphologic changes of lens epithelial cells. Another 4 rats were irradiated with ultraviolet B for 40 days. The lens images of 8 eyes were collected simultaneously by three methods, and the results of the three methods were compared and analyzed. The result is 1: 1. The results of experimental group showed that SD rats were exposed to UVB for 2 hours a day. On the 5th day, the lens remained transparent and had no obvious opacity, with the prolongation of irradiation time. On the 20th day, the cortex was slightly turbid, mostly located near the "Y" syringe, showing the anterior flake, feathery towards the equatorial part of the superficial cortical opacity, the color was white, and there was no change in the transparency and color of the lens nucleus. At 40 days, the degree of anterior subcapsular cortex opacity was more obvious, and white turbidity could be observed in the anterior superficial cortical layer and deep cortical layer, and the range could be extended to the near equatorial part. When the anterior capsule membrane was removed, the adhesion between the superficial cortex and the anterior capsule membrane of most of the rats was closer than that of the normal group. No significant color changes were found in the lens nucleus of the irradiation group after 40 days of irradiation. In the control group, there was no obvious turbidity and color change in the lens cortex and nucleus, and the transparency was maintained at the same time. Apoptosis of lens epithelial cells in vivo exposed to UV irradiation for 5 days, apoptosis of lens anterior capsule epithelial cells was observed, and apoptotic cells were found to be brown. 3. Morphological changes: the size of lens epithelial cells in the group of: 5 days was not significantly changed compared with that in the control group. A large number of lens fibers were connected with lens epithelial cells in the group of 40 days. Three different methods of lens acquisition were used to obtain the lens image of the same cataract lens. Compared with the other two methods, the image collected by the lens support plate combined with the anterior segment photography system in this experiment is clearer and more stable than that obtained by the other two methods. Conclusion 1. This method successfully realized the establishment of ultraviolet radiation induced cataract rat model. 2. 2. Ultraviolet B is one of the causes of cataract. The degree of lens opacity was positively correlated with the duration of ultraviolet B irradiation. Ultraviolet B irradiation induced apoptosis of lens anterior capsule epithelial cells in SD rats. The three methods of lens image acquisition have their own advantages and disadvantages. In this experiment, an ideal lens image can be obtained by using black lens plate combined with anterior segment photography system, which has obvious advantages compared with other two methods.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R776.1

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