發(fā)布時(shí)間：2018-04-18 16:01

  本文選題：過(guò)氧化物酶體增殖物激活受體γ + 激動(dòng)劑��； 參考：《武漢大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2014年06期

【摘要】：目的:探討過(guò)氧化物酶體增殖物激活受體γ(PPARγ)激動(dòng)劑對(duì)角膜新生血管的抑制作用及機(jī)制。方法:SD大鼠隨機(jī)分成A、B、C及D組,行角膜囊袋法制作角膜新生血管模型,右眼為實(shí)驗(yàn)眼。A組:空白對(duì)照組,正常角膜大鼠;B:陽(yáng)性對(duì)照組,制作模型后生理鹽水滴眼;C、D組:建模后分別滴用0.5%、1.0%PPARγ激動(dòng)劑滴眼液。裂隙燈顯微鏡下測(cè)量新生血管面積,Western blot檢測(cè)角膜血管內(nèi)皮生長(zhǎng)因子(VEGF)表達(dá),ELISA檢測(cè)房水VEGF的表達(dá)變化。結(jié)果:建模后第3,7,14,21,28天,角膜新生血管面積B組為(5.53±0.81)、(14.61±2.78)、(26.12±2.63)、(34.74±3.56)、(37.42±3.87)mm2,C組為(3.75±1.08)、(10.36±1.27)、(18.76±2.35)、(27.88±3.06)、(29.73±3.54)mm2,D組從第7天開(kāi)始為(3.82±0.94)、(8.74±1.06)、(13.93±2.06)、(14.61±2.82)mm2。28dC、D組角膜新生血管抑制率分別為20.6%和61.0%,D組大鼠角膜新生血管面積小于B組和C組,差異有統(tǒng)計(jì)學(xué)意義(P0.05),而C組與B組之間差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。Western blot檢測(cè)燒傷后14d新生血管角膜表達(dá)VEGF明顯增強(qiáng),C、D組VEGF相對(duì)量有不同程度下降。角膜損傷后,大鼠房水中VEGF表達(dá)隨時(shí)間明顯增多,高濃度的PPARγ激動(dòng)劑滴眼液治療后能明顯降低房水VEGF的含量,第21天D組房水VEGF的表達(dá)明顯低于B組[(687±126)pg/ml vs(1 260±104)pg/ml],差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:局部應(yīng)用PPARγ激動(dòng)劑能有效抑制角膜新生血管生成,抑制VEGF的合成和表達(dá)可能是阻止角膜新生血管生長(zhǎng)機(jī)制的一部分。
[Abstract]:Aim: to investigate the inhibitory effect and mechanism of peroxisome proliferator activated receptor (PPAR 緯) agonist on corneal neovascularization.Methods Twenty SD rats were randomly divided into two groups: group C and group D, and the corneal neovascularization model was made with the method of corneal capsule. The right eye was divided into experimental eye (group A): blank control group (group A) and normal cornea group (group B: positive control group).After the establishment of the model, normal saline drops were used in group C: after modeling, 0.5 and 1.0% PPAR 緯 agonist eye drops were used respectively.Measurement of neovascularization area under slit lamp microscope Western blot was used to detect the expression of corneal vascular endothelial growth factor (VEGF). Elisa was used to detect the expression of VEGF in aqueous humor.緇撴灉:寤烘ā鍚庣3,7,14,21,28澶，

本文編號(hào)：1769062