本文選題：bFGF　切入點(diǎn)：uPA　出處：《廣西醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的:應(yīng)用免疫組織化學(xué)方法,對bFGF和uPA在不同TNM分期及臨床分期鼻咽癌(Nasopharyngeal Carcinoma,NPC)組織中的表達(dá)情況進(jìn)行檢測,分析其與臨床病理因素和患者治療后生存時(shí)間的關(guān)系。探討它們在鼻咽癌發(fā)生發(fā)展過程中的作用,為鼻咽癌的早期診斷、預(yù)后判斷提供新的理論基礎(chǔ)。 方法:1.應(yīng)用SupervisionTM二步法分別檢測具有完整臨床資料155例NPC組織和20例鼻咽粘膜慢性炎組織中bFGF、uPA蛋白表達(dá)情況。2.采用SPSS13.0統(tǒng)計(jì)軟件進(jìn)行數(shù)據(jù)處理,以P0.05為顯著性水準(zhǔn)。用χ2檢驗(yàn)分析bFGF和uPA蛋白表達(dá)與NPC患者臨床病理特征之間的關(guān)系;Spearman等級相關(guān)分析法分析兩者之間相關(guān)性;Kaplan-Meier法進(jìn)行單因素生存分析,生存曲線比較用Log-Rank檢驗(yàn)。 結(jié)果:1. bFGF和uPA蛋白在NPC組織中的陽性表達(dá)率分別為80.6%和79.4%,在對照組織中基本不表達(dá),bFGF和uPA蛋白在NPC和鼻咽粘膜慢性炎組織中陽性表達(dá)率的差異有統(tǒng)計(jì)學(xué)意義(P0.01)。2. bFGF和uPA蛋白在NPC表達(dá)與性別、年齡均無相關(guān)性(P0.05)。3. bFGF和uPA蛋白陽性表達(dá)率在NPC臨床TNM分期的T1、T2、T3、T4分別為:53.3%、75.9%、84.2%、96.6%和53.3%、66.7%、91.2%、93.1% ,兩指標(biāo)在各自T期總體比較均有非常顯著統(tǒng)計(jì)學(xué)意義(均為P0.01);在N0、N1、N2、N3分別為77.8%、69.8%、80.9%、94.7%和70.4%、69.8%、82.3%、92.1%,兩指標(biāo)在各自N期總體比較均有顯著統(tǒng)計(jì)學(xué)意義(均為P0.05)。在M0、M1分別為78.0%、95.7%和76.5%、95.7%,兩指標(biāo)兩組之間比較均有顯著統(tǒng)計(jì)學(xué)意義(均為P0.05)。4. bFGF和uPA蛋白陽性表達(dá)率在臨床分期為Ⅰ~Ⅱ、Ⅲ、Ⅳa、Ⅳb期分別為43.3%、87.7%、88.9%、95.7%和43.3%、84.2%、88.9%、95.7%,兩指標(biāo)在各自臨床分期總體比較均有非常顯著統(tǒng)計(jì)學(xué)意義(均為P0.01)。5. bFGF和uPA蛋白在鼻咽癌表達(dá)呈明顯正相關(guān)(r=0.557,P=0.000)。6.單因素生存分析提示, bFGF和uPA蛋白單獨(dú)或聯(lián)合表達(dá)陽性患者治療后中位生存時(shí)間短于陰性患者。存在淋巴結(jié)轉(zhuǎn)移、遠(yuǎn)處轉(zhuǎn)移,TNM分期晚期的患者提示有不良預(yù)后。 結(jié)論:1.鼻咽癌組中bFGF和uPA蛋白陽性的表達(dá)率明顯高于炎癥組,提示兩基因可能參與腫瘤發(fā)生發(fā)展過程中。2. bFGF和uPA蛋白表達(dá)與性別、年齡無關(guān),而與NPC的TNM分期和臨床分期均有非常密切的關(guān)系,表明bFGF和uPA是NPC的局部浸潤轉(zhuǎn)移、淋巴結(jié)轉(zhuǎn)移和遠(yuǎn)處器官轉(zhuǎn)移的重要參與因子。3. bFGF和uPA蛋白表達(dá)呈顯著正相關(guān),表明它們很可能為NPC浸潤轉(zhuǎn)移的共同促進(jìn)因子。4. bFGF和uPA與NPC的預(yù)后有關(guān),提示bFGF和uPA陽性表達(dá)的NPC患者生存期短,預(yù)后差;二者聯(lián)合表達(dá)有降低NPC患者生存時(shí)間的危險(xiǎn)。
[Abstract]:Objective: to detect the expression of bFGF and uPA in nasopharyngeal carcinoma (NPC) with different TNM stages and clinical stages by immunohistochemical method, and to analyze the relationship between the expression of uPA and clinicopathological factors and survival time after treatment.To explore their role in the development of nasopharyngeal carcinoma and provide a new theoretical basis for the early diagnosis and prognosis of nasopharyngeal carcinoma.Method 1: 1.The expression of bFGF UPA protein in 155 cases of NPC and 20 cases of chronic nasopharyngeal mucositis was detected by SupervisionTM two-step method.Using SPSS13.0 statistical software for data processing, P0.05 as the significant level.The relationship between the expression of bFGF and uPA protein and the clinicopathological characteristics of NPC patients was analyzed by 蠂 2 test. The correlation between them was analyzed by Kaplan-Meier method. The survival curve was compared with Log-Rank test.Results the positive expression rates of bFGF and uPA in NPC were 80.6% and 79.4%, respectively. There was significant difference in the positive expression rate of NPC and uPA between NPC and chronic nasopharyngeal mucositis. BFGFAnd uPA protein expression and sex in NPC,The positive expression rate of bFGF and uPA protein in clinical TNM stage of NPC was 75.90% and 84.2%, respectively. The positive expression rate of bFGF and uPA protein in NPC clinical TNM stage was 93.1% and 91.2%, respectively.涓ゆ寚鏍囧湪鍚勮嚜T鏈熸，

本文編號：1707770