本文選題：前庭　切入點：石蠟連續(xù)切片　出處：《大連醫(yī)科大學(xué)》2011年碩士論文

【摘要】：目的: 1.本實驗通過組織石蠟連續(xù)切片,結(jié)合計算機技術(shù)得到豚鼠內(nèi)耳前庭膜迷路的三維幾何模型,可清晰顯示內(nèi)耳前庭器官:膜半規(guī)管、橢圓囊及球囊等的形態(tài)結(jié)構(gòu),加深臨床醫(yī)生對內(nèi)耳前庭宏細(xì)觀結(jié)構(gòu)的認(rèn)識和準(zhǔn)確量化理解,為建立異常條件下的動物實驗?zāi)Ｐ图皯?yīng)用于人體實驗奠定基礎(chǔ)。 2.熟練掌握石蠟連續(xù)切片技術(shù)及計算機三維重建的方法,為內(nèi)耳前庭各結(jié)構(gòu)參數(shù)的精確測量和評價,以及生物力學(xué)數(shù)值模型的建立提供有效的工具。 3.對豚鼠內(nèi)耳前庭三維重建模型進行溫度場的數(shù)值模擬,分析溫度刺激下前庭系統(tǒng)膜迷路內(nèi)的生物力學(xué)響應(yīng)。結(jié)合臨床雙溫試驗,有助于定量了解前庭系統(tǒng)維持平衡功能的機理,為前庭性疾病的診療提供一定的參考依據(jù)。 方法:采用新鮮豚鼠顳骨,經(jīng)過固定、脫鈣、脫水、透明、浸蠟、包埋定位、切片機連續(xù)切片、染色、封片等,獲得了片厚6μm的石蠟連續(xù)切片一套,采用數(shù)碼攝像機進行采集,獲取橫斷面二維圖像數(shù)字信息,利用計算機技術(shù)進行三維重建,建立包含膜半規(guī)管、橢圓囊、球囊等細(xì)微結(jié)構(gòu)的內(nèi)耳前庭三維模型。基于三維成像模型進行數(shù)據(jù)測量,運用有限元方法進行內(nèi)耳前庭溫度場的數(shù)值模擬。 結(jié)果: 1.獲得了一套完整的豚鼠內(nèi)耳組織連續(xù)切片及放大后圖像資料。證實了從豚鼠內(nèi)耳取材至切片、封片的實驗步驟均具有可行性。 2.利用石蠟連續(xù)切片方法結(jié)合計算機技術(shù),建立了豚鼠內(nèi)耳前庭膜性結(jié)構(gòu)的三維模型,可清楚的顯示豚鼠內(nèi)耳前庭的細(xì)微結(jié)構(gòu)及空間特性,并得到了內(nèi)耳前庭的三維數(shù)據(jù)。 3.對豚鼠內(nèi)耳前庭三維成像模型進行溫度場的數(shù)值模擬,顯示了在溫度刺激下前庭系統(tǒng)內(nèi)復(fù)雜的力學(xué)行為。本文中給出了△T=+1℃、△T=+7℃及△T=-1℃三種溫度刺激,觀察隨著時間的推移引起半規(guī)管壺腹嵴嵴頂?shù)奈灰�、速度及壓強的變化。溫度升高與降低時,位移均逐漸增大,速度均逐漸減小;溫度升高,壓強逐漸增大,溫度降低,壓強逐漸減小,以上指標(biāo)最終均趨于一個穩(wěn)定值。溫差越大,力學(xué)響應(yīng)越大。觀察三個半規(guī)管同一溫度刺激下嵴頂?shù)奈灰祈憫?yīng)得出:外半規(guī)管壺腹嵴嵴頂?shù)奈灰祈憫?yīng)最大,兩垂直半規(guī)管壺腹嵴嵴頂?shù)奈灰祈憫?yīng)相對較小,且后半規(guī)管壺腹嵴嵴頂?shù)奈灰祈憫?yīng)是最小的。壓強云圖上可見壓強降主要集中在外半規(guī)管。說明溫度干預(yù)對外半規(guī)管影響較大。 結(jié)論: 1.利用組織連續(xù)切片技術(shù)獲取二維圖像數(shù)字信息并進行計算機豚鼠內(nèi)耳前庭三維重建,既具備了切片的細(xì)節(jié)性,又能反映其空間特性,而且可以進行三維數(shù)據(jù)測量,是一種提高顳骨形態(tài)學(xué)研究水平的方法,為今后的形態(tài)學(xué)教學(xué)及研究奠定了基礎(chǔ)。 2.通過對內(nèi)耳前庭溫度場的數(shù)值模擬分析,能夠定量了解前庭系統(tǒng)維持平衡的機理,為評價半規(guī)管及整個前庭系統(tǒng)的功能狀態(tài)提供了一定的數(shù)據(jù)參考。 3.采用石蠟連續(xù)切片方法結(jié)合計算機技術(shù)用于內(nèi)耳前庭宏細(xì)觀結(jié)構(gòu)三維重建并進行數(shù)值模擬的方法具有可行性。
[Abstract]:Objective:
1. this experiment through the paraffin serial sections, combined with computer technology the three-dimensional geometric model of guinea pig vestibular membrane lost, can clearly show the vestibular organs: the morphology and structure of membrane canal, utricular and saccular etc., deepen clinicians in the vestibular macro meso structure understanding and accurate quantitative understanding, to establish an animal model of abnormal under the condition of the human experiment and application to lay the foundation.
2., we have a good command of paraffin continuous slice technology and computer three-dimensional reconstruction method, providing an effective tool for the accurate measurement and evaluation of the structural parameters of the inner ear vestibule, and the establishment of a biomechanical numerical model.
Numerical simulation of temperature field of 3. in guinea pig vestibular 3D model, biomechanical analysis of temperature under the stimulation of the vestibular system film lost in response. Combined with clinical trials of double temperature mechanism contributes to quantitatively understand the balance function of the vestibular system, and to provide reference for diagnosis and treatment of vestibular disease.
Methods: the fresh guinea pig bone, after fixed, decalcified, dehydrated, transparent, paraffin embedding, serial sectioning, positioning, slicing machine staining, mounting, the thickness of 6 m paraffin serial sections of a digital camera, the acquisition, acquisition of digital cross sectional two-dimensional image information reconstructed using computer technology includes the establishment of the utricle, membranous semicircular canal, vestibular 3D fine structure. The balloon model based on 3D imaging measurement data, using the finite element method to simulate the temperature field of the vestibule.
Result:
1. get a complete set of inner ear serialsections and enlarge image data. Confirmed the story from the inner ear to the mounting section, experimental steps are feasible.
2., a three-dimensional model of the inner ear vestibule membrane structure of guinea pig was established by paraffin continuous slice method combined with computer technology. It can clearly show the fine structure and spatial characteristics of the inner ear vestibule of the guinea pig, and get the three-dimensional data of the inner ear vestibule.
Numerical simulation of temperature field of 3. in vestibule of guinea pig 3D imaging model, shows the complicated mechanical behavior under temperature stimulation in the vestibular system. This paper gives a T=+1 C, C and C delta T=+7 Delta T=-1 three temperature stimulation, observed with the passage of time caused by displacement of the semicircular canal crista crest, change the speed and pressure. The temperature rises and decreases, the displacement increase gradually, the speed decreases gradually; temperature increases, the pressure increases, the decrease of temperature, pressure decreases gradually, the above indexes are finally reached a stable value. The higher the temperature is, the greater the mechanical response. The observation that the same temperature stimulation under the crest displacement the response of the three semicircular canals: lateral displacement of the crista crest response maximum displacement, two vertical semicircular canal crista crest response is relatively small, and the displacement of the semicircular canal crista crest response pressure is minimal. The pressure drop is mainly concentrated in the outer semicircular canals, indicating that temperature intervention has a great influence on the external semicircular canals.
Conclusion:
1. use the serialsections technique to obtain two-dimensional image information and computer three-dimensional reconstruction of vestibule of guinea pig, not only has the details of the slice, and can reflect the space characteristics, but also can be used for 3D data measurement, is a method to improve the morphology of the temporal method of water level, which laid the foundation for the future research and teaching.
2., through the numerical simulation analysis of the vestibular temperature field in the inner ear, we can understand quantitatively the mechanism of maintaining the balance of the vestibular system, and provide some data reference for evaluating the functional state of semicircular canal and the whole vestibular system.
3. the method of continuous paraffin section combined with computer technology for three-dimensional reconstruction and numerical simulation of the macro mesoscopic structure of the vestibule of the inner ear is feasible.

【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R764

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本文編號：1654411