本文選題：視網(wǎng)膜神經(jīng)節(jié)細胞　切入點：急性高眼壓　出處：《眼科新進展》2014年08期 　論文類型：期刊論文

【摘要】：目的探討表皮生長因子受體(epidermal growth factor receptor,EGFR)抑制劑抑制大膠質(zhì)細胞活化對實驗性急性高眼壓大鼠視網(wǎng)膜神經(jīng)節(jié)細胞(retinal ganglion cells,RGCs)丟失的影響。方法用健康成年SD大鼠18只,隨機分為3組:空白對照組,不做任何處理;實驗組采用前房灌注法制造急性高眼壓模型,造模成功后,按6 mg·kg-1·d-1用量給予大鼠口服EGFR抑制劑AG1478;實驗對照組造模成功后給予大鼠口服等量生理鹽水。3組均于7 d處死大鼠,觀察各組視網(wǎng)膜結(jié)構(gòu)的變化及采用免疫組織化學(xué)法觀察陽性節(jié)細胞的表達。結(jié)果通過免疫組化法發(fā)現(xiàn),隨著造模時間的延長,視網(wǎng)膜結(jié)構(gòu)變得模糊不清,RGCs陽性染色細胞逐漸減少�？瞻讓φ战M、實驗組、實驗對照組RGCs Thy-1陽性表達光密度值分別是:143.666 7±4.041 5、139.032 2±1.734 0和101.102 3±2.000 1。實驗組和空白對照組相比,視網(wǎng)膜Thy-1陽性表達差異無統(tǒng)計學(xué)意義(P0.05),實驗對照組和空白對照組比較,差異有統(tǒng)計學(xué)意義(P0.05),實驗組與實驗對照組視網(wǎng)膜Thy-1陽性表達,差異亦有統(tǒng)計學(xué)意義(P0.05)。結(jié)論 EGFR抑制劑通過抑制大膠質(zhì)細胞的活化對實驗性急性高眼壓大鼠模型的RGCs有保護作用。
[Abstract]:Objective to investigate the effect of epidermal growth factor receptor (EGFR) inhibitor on the loss of retinal ganglion cells and retinal ganglion cells (RGCs) in experimental acute intraocular hypertension rats. Methods 18 adult Sprague-Dawley (SD) rats were used to investigate the effects of epidermal growth factor receptor inhibitor (EGFR) on the loss of retinal ganglion cells (RGCs) in rats with acute intraocular hypertension (AIOP). Three groups were randomly divided into three groups: the blank control group without any treatment, the experimental group using the anterior chamber perfusion method to make the acute intraocular pressure model, after the model was successfully made, Rats were given EGFR inhibitor AG1478 at the dosage of 6 mg 路kg-1 路d-1, and rats in the control group were killed at 7 days after the rats were given the same amount of saline. The changes of retinal structure and the expression of positive ganglion cells were observed by immunohistochemical method. The optical density of RGCs Thy-1 positive expression in the blank control group, experimental group and experimental control group was 143.6667 鹵4.041 5,139.032 2 鹵1.734 0 and 101.102 3 鹵2.000 1 respectively. There was no significant difference in the positive expression of Thy-1 between the experimental group and the control group (P 0.05). There was a significant difference between the experimental control group and the blank control group (P 0.05). The positive expression of Thy-1 in the retina of the experimental group and the experimental control group was positive. Conclusion EGFR inhibitor has protective effect on RGCs of experimental acute intraocular hypertension rat model by inhibiting the activation of macroglia cells.
【作者單位】： 武漢大學(xué)附屬武漢市第三醫(yī)院眼科;中南大學(xué)附屬湘雅二醫(yī)院眼科;
【基金】：湖南省自然科學(xué)基金資助(編號:10JJ6031) 湖南省科技廳基金資助(編號:2009-130)~~
【分類號】：R775

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