發(fā)布時(shí)間：2018-03-11 03:17

  本文選題：血紅素氧合酶-1　切入點(diǎn)：視網(wǎng)膜　出處：《蘭州大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 目的通過制備大鼠視網(wǎng)膜光損傷的動(dòng)物模型,觀察并分析血紅素氧合酶-1(HO-1)對(duì)大鼠視網(wǎng)膜光損傷時(shí)的細(xì)胞保護(hù)作用。 方法分別以綠色熒光燈為光源制備光照組模型及光照前30min腹腔注射血晶素(Hemin)制備誘導(dǎo)組模型,以正常大鼠作為對(duì)照組,取大鼠視網(wǎng)膜組織,免疫組織化學(xué)染色方法觀察HO-1在各組大鼠視網(wǎng)膜組織中的表達(dá)情況,以及通過流式細(xì)胞術(shù)檢測(cè)各組大鼠視網(wǎng)膜細(xì)胞的凋亡率。 結(jié)果光照組大鼠在接受光照后較正常對(duì)照組大鼠視網(wǎng)膜HO-1的表達(dá)有所增加(p0.05),第一天即有表達(dá),第五天時(shí)達(dá)高峰,隨后逐漸下降,而其視網(wǎng)膜細(xì)胞的凋亡率表現(xiàn)出相同的變化趨勢(shì)。光照前腹腔注射血晶素(Hemin)的誘導(dǎo)組大鼠視網(wǎng)膜HO-1的表達(dá)明顯增高,且各時(shí)間點(diǎn)(1d、3d、5d、7d)的表達(dá)量均高于光照組(p0.05),而其視網(wǎng)膜細(xì)胞的凋亡率較光照組下降,同時(shí)間點(diǎn)(1d、3d、5d、7d)凋亡率均低于光照組(p0.05),而高于正常對(duì)照組(p0.05)。 結(jié)論血紅素氧合酶-1對(duì)光損傷時(shí)的視網(wǎng)膜具有明顯的細(xì)胞保護(hù)作用,且其表達(dá)量的水平受其誘導(dǎo)劑的影響而增加。
[Abstract]:Objective to observe and analyze the protective effect of heme oxygenase-1 (HO-1) on retinal light injury in rats. Methods the model of light group was made with green fluorescent lamp and the model of induced group was induced by intraperitoneal injection of hemagglutinin Heminin 30 minutes before illumination. The normal rats were used as the control group and the retinal tissues of the rats were taken out. Immunohistochemical staining was used to observe the expression of HO-1 in the retina of rats in each group and the apoptosis rate of retinal cells in each group was detected by flow cytometry. Results the expression of HO-1 in the retina of the illumination group was higher than that of the normal control group. The expression of HO-1 increased on the first day, reached its peak at 5th days, and then decreased gradually. The apoptosis rate of retinal cells showed the same change trend. The expression of HO-1 in retina of rats induced by intraperitoneal injection of hemagglutinin before illumination was significantly increased. The apoptotic rate of retinal cells was lower than that of light group, and the apoptotic rate of retinal cells was lower than that of light group at the same time. The apoptotic rate of retina cells was lower than that of light group (P 0.05), but higher than that of normal control group (P 0.05). Conclusion heme oxygenase-1 has a significant cytoprotective effect on retina after light injury, and its expression level is increased by its inducer.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類號(hào)】：R774.13

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 謝伯林,王正國,朱佩芳,嚴(yán)密,張軍軍;大鼠視網(wǎng)膜光化學(xué)損傷的病理特征[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2000年05期

2 孔祥斌;謝伯林;朱麗;鄧海波;;不同光照射方式對(duì)大鼠視網(wǎng)膜外核層厚度的影響[J];國際眼科雜志;2007年06期

3 劉學(xué)政,于樹春,李永洋;實(shí)驗(yàn)性大鼠視網(wǎng)膜光損傷模型的建立與研究[J];錦州醫(yī)學(xué)院學(xué)報(bào);2002年06期

4 韓文杰,牛膺筠;低氧預(yù)適應(yīng)與視網(wǎng)膜光損傷的防治研究進(jìn)展[J];臨床眼科雜志;2004年04期

5 張雁林;趙金垣;;血紅素加氧酶-1的細(xì)胞保護(hù)作用研究進(jìn)展[J];環(huán)境與職業(yè)醫(yī)學(xué);2008年02期

6 范俊萍,彭清;維生素C、維生素E對(duì)視網(wǎng)膜光損傷防護(hù)作用的研究[J];山西醫(yī)科大學(xué)學(xué)報(bào);2001年02期

7 徐克成,葉平;正鐵血紅素治療急性間歇性血卟啉癥[J];胃腸病學(xué)和肝病學(xué)雜志;1999年03期

8 徐建平;血紅素加氧酶系統(tǒng)與氧化應(yīng)激[J];國外醫(yī)學(xué)(生理、病理科學(xué)與臨床分冊(cè));2002年04期

9 馬鋒偉;周占宇;豐慧;付浴東;;光損傷實(shí)驗(yàn)性大鼠視網(wǎng)膜組織中survivin表達(dá)的變化[J];眼科新進(jìn)展;2010年01期

10 文峰;李海濤;陳艷麗峰;孫祖峰;;黃芩素與輔酶Q_(10)對(duì)大鼠視網(wǎng)膜光損傷防護(hù)作用的研究[J];眼科研究;2006年05期

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