本文選題：糖尿病視網(wǎng)膜病變　切入點(diǎn)：血紅素加氧酶-　出處：《眼科新進(jìn)展》2014年01期 　論文類型：期刊論文

【摘要】：目的觀察血紅素加氧酶-1(heme oxygenase-1,HO-1)在糖尿病視網(wǎng)膜病變(dia betic retinopathy,DR)患者外周血單個(gè)核細(xì)胞(peripheral blood mono-nuclear cell,PBMC)中的表達(dá)。方法選擇糖尿病病程大于6個(gè)月的2型糖尿病患者共64例,根據(jù)臨床表現(xiàn)和診斷將其分為無(wú)糖尿病視網(wǎng)膜病變(non-diabetic retinopathy,NDR)組18例及DR組46例其中DR組中包括非增生型DR(non-proliferative diabetic retinopathy,NPDR)患者24例及增生型DR(proliferative retinopathy,PDR)患者22例。選擇同期行體檢的健康志愿者20人作為對(duì)照。采用實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(quantitative real-time polymerase chain reac tion,qRT-PCR)檢測(cè)各組PBMC中HO-1 mRNA的表達(dá)水平,比較各組之間HO-1 mRNA表達(dá)水平的差異,并分析HO-1 mRNA表達(dá)水平與各項(xiàng)理化指標(biāo)之間的相關(guān)性。結(jié)果 qRT PCR檢測(cè)發(fā)現(xiàn),DR組、NDR組以及對(duì)照組PBMC中HO-1 mRNA相對(duì)表達(dá)量分別是0.015±0.007、0.021±0.010、0.027±0.013,差異有統(tǒng)計(jì)學(xué)意義(F=10.888,P0.001)。其中,DR組HO-1 mRNA的相對(duì)表達(dá)量明顯低于NDR組(P=0.014);NDR組與對(duì)照組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.125);PDR組患者PBMC中HO-1 mRNA相對(duì)表達(dá)量為0.012±0.007,顯著低于NPDR組(0.018±0.007)(t=-3.052,P=0.004)。ln(HO-1)mRNA表達(dá)水平與收縮壓(β=-0.011,P=0.001)及糖化血紅蛋白(β=0.088,P=0.008)之間存在顯著相關(guān)性。結(jié)論 HO-1 mRNA在DR患者及PDR患者PBMC中的表達(dá)顯著降低,提示HO-1可能作為一個(gè)保護(hù)性因素參與DR的發(fā)生及病情進(jìn)展。
[Abstract]:Objective to investigate the expression of heme oxygenase-1 (HO-1) in peripheral blood mononuclear cells (PBMC) of patients with diabetic retinopathy (betic). According to the clinical manifestation and diagnosis, it was divided into 18 cases of non-diabetic retinopathy group and 46 cases of Dr group, including 24 cases of non-proliferative DR(non-proliferative diabetic retinopathy and 22 cases of proliferative DR(proliferative retinopathy. Quantitative real-time polymerase chain reac qRT-PCRs were used to detect the expression of HO-1 mRNA in PBMC of 20 healthy volunteers. To compare the expression level of HO-1 mRNA between groups, Results the relative expression of HO-1 mRNA in PBMC of Dr group and control group was 0.015 鹵0.007 鹵0.021 鹵0.010 鹵0.027 鹵0.013 respectively, the difference was statistically significant (P 0.001). The relative expression of HO-1 mRNA in PBMC of NDR group was 0.012 鹵0.007, which was significantly lower than that of NDR group (0.018 鹵0.007), and was significantly lower than that of NPDR group (0.018 鹵0.007 ~ 3.052P 0.004. LnHO-1 mRNA) and systolic pressure (尾 -0.011 P0. 001) and glycosylated hemoglobin (尾 -0.08P0. 008). Conclusion the expression of HO-1 mRNA in PBMC of Dr patients and PDR patients was significantly decreased. The results suggest that HO-1 may be a protective factor in the pathogenesis and progression of Dr.
【作者單位】： 南方醫(yī)科大學(xué);廣東省眼病防治研究所 廣東省人民醫(yī)院眼科;廣東省醫(yī)學(xué)科學(xué)院;廣東省人民醫(yī)院醫(yī)學(xué)研究部;
【基金】：國(guó)家自然科學(xué)基金資助(編號(hào):81371031) 廣東省醫(yī)學(xué)科研基金資助(編號(hào):B2011013) 廣州市珠江科技新星專項(xiàng)基金資助(編號(hào):2011J2200050)~~
【分類號(hào)】：R587.1;R774.1

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