本文選題：喉癌　切入點：Brg1　出處：《吉林大學》2010年博士論文　論文類型：學位論文

【摘要】： 本研究通過分析喉鱗癌組織中Brg1蛋白的表達,粉防己堿(Tet)對喉鱗狀細胞癌細胞系中Brg1及相關基因表達的影響,探討B(tài)rg1基因與喉鱗癌發(fā)生發(fā)展的關系,以及Tet的抗腫瘤機制。采用免疫組化方法分析Brg1在喉鱗癌組織中的表達,比較其在正常喉粘膜、聲帶息肉、喉癌前病變及喉鱗狀細胞癌中的表達情況。采用免疫熒光法檢測Tet對Hep-2細胞中Brg1表達的影響,采用MTT法檢測不同濃度的Tet對喉鱗癌Hep-2細胞增殖的影響,采用流式細胞儀檢測不同濃度Tet對細胞周期的影響,以及應用fluo-3檢測Tet對細胞內鈣離子濃度的影響,表明在喉鱗狀細胞癌中Brg1表達下降,在喉癌發(fā)展過程中有重要作用。Tet可使Hep-2細胞中Brg1表達增強,抑制腫瘤細胞生長,并可使Hep-2細胞內鈣離子濃度降低。采用免疫熒光法檢測Tet對Hep-2細胞中受Brg1調節(jié)的鈣調蛋白AHNAK的表達,并進一步應用實時RT-PCR方法檢測細胞內Brg1和AHNAK mRNA水平,結果發(fā)現(xiàn)Tet可使Hep-2細胞內Brg1和AHNAK轉錄增強。表明Tet可使受Brg1調控的鈣調蛋白AHNAK的表達增強,Brg1的表達可能與細胞內鈣相關。Brg1作為一種抑癌基因在喉鱗狀細胞癌中表達下降,可能與喉癌的發(fā)生密切相關。Tet為一中藥抗腫瘤藥物,可使Hep-2細胞中Brg1表達增強,其抗腫瘤作用可能通過影響B(tài)rg1表達而實現(xiàn)。
[Abstract]:By analyzing the expression of Brg1 protein in laryngeal squamous cell carcinoma and the effect of Tetrandrine on the expression of Brg1 and related genes in laryngeal squamous cell carcinoma cell line, the relationship between Brg1 gene and the occurrence and development of laryngeal squamous cell carcinoma was studied. Immunohistochemical method was used to analyze the expression of Brg1 in laryngeal squamous cell carcinoma (LSCC), and to compare the expression of Brg1 in normal laryngeal mucosa and vocal cord polyp. The effect of Tet on the expression of Brg1 in Hep-2 cells was detected by immunofluorescence assay, and the effect of different concentrations of Tet on the proliferation of Hep-2 cells in laryngeal squamous cell carcinoma (LSCC) was detected by MTT assay. Flow cytometry was used to detect the effect of different concentrations of Tet on cell cycle, and fluo-3 was used to detect the effect of Tet on intracellular calcium concentration, which indicated that the expression of Brg1 decreased in laryngeal squamous cell carcinoma. Tet plays an important role in the development of laryngeal carcinoma. Tet can enhance the expression of Brg1 in Hep-2 cells and inhibit the growth of tumor cells. Immunofluorescence assay was used to detect the expression of calmodulin AHNAK regulated by Brg1 in Hep-2 cells by Tet, and the levels of Brg1 and AHNAK mRNA in Hep-2 cells were detected by real-time RT-PCR method. The results showed that Tet could enhance the expression of Brg1 and AHNAK in Hep-2 cells, suggesting that Tet could enhance the expression of Brg1 regulated calmodulin AHNAK and the expression of Brg1 might be related to the decrease of intracellular Ca2 + related. Brg1 as a tumor suppressor gene in laryngeal squamous cell carcinoma. It may be closely related to the occurrence of laryngeal carcinoma. Tet is a traditional Chinese medicine anti-tumor drug, which can enhance the expression of Brg1 in Hep-2 cells, and its anti-tumor effect may be realized by affecting the expression of Brg1.
【學位授予單位】：吉林大學
【學位級別】：博士
【學位授予年份】：2010
【分類號】：R739.65

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