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  本文選題：視網(wǎng)膜缺血再灌注損傷　切入點：骨髓間充質(zhì)干細胞移植　出處：《眼科新進展》2014年04期 　論文類型：期刊論文

【摘要】：目的探討骨髓間充質(zhì)干細胞(bone marrow mesenchymal stem cells,BMSC)移植對大鼠視網(wǎng)膜缺血再灌注損傷(retinal ischemia reperfusion injury,RIRI)凋亡相關因子Fas和FasL表達的影響。方法體外貼壁篩選法培養(yǎng)大鼠BMSC。將39只健康成年SD大鼠隨機分成正常組3只、模型組18只、BMSC移植組18只。各組大鼠均選擇右眼作為實驗眼建立大鼠RIRI模型,正常組不做任何處理。另2組大鼠于RIRI后1 h分別給予玻璃體腔注射PBS緩沖液(模型組)、BMSC(BMSC移植組),并于RIRI后2 h、6 h、12 h、24 h、48 h、72 h各隨機處死3只SD大鼠,迅速摘取右眼。免疫組織化學法檢測大鼠視網(wǎng)膜組織Fas和FasL的陽性細胞數(shù)情況。結果正常視網(wǎng)膜組織中Fas極少表達,FasL視網(wǎng)膜全層低表達。RIRI后2 h、6 h、12 h、24 h、48 h、72 h,模型組Fas陽性細胞數(shù)分別為(20.5±0.9)個·mm-2、(263.6±15.6)個·mm-2、(583.5±15.8)個·mm-2、(1184.6±45.5)個·mm-2、(684.5±3.5)個·mm-2、(80.6±4.5)個·mm-2,BMSC移植組分別為(19.8±0.8)個·mm-2、(238.5±14.7)個·mm-2、(473.6±13.6)個·mm-2、(964.5±23.7)個·mm-2、(632.6±5.8)個·mm-2、(72.5±6.3)個·mm-2;模型組FasL陽性細胞數(shù)分別為(42.2±0.9)個·mm-2、(57.3±0.9)個·mm-2、(365.8±7.5)個·mm-2、(1004.3±17.9)個·mm-2、(793.5±8.6)個·mm-2、(210.6±4.9)個·mm-2,BMSC移植組分別為(40.5±0.8)個·mm-2、(55.6±0.7)個·mm-2、(351.2±6.8)個·mm-2、(992.8±6.0)個·mm-2、(683.4±12.9)個·mm-2、(206.3±4.7)個·mm-2。與模型組比較,BMSC移植組各時間點Fas、FasL的陽性細胞數(shù)明顯減少。Fas在6~48 h各組差異有統(tǒng)計學意義(P0.05),FasL在12~48 h各組差異有統(tǒng)計學意義(P0.05)。結論 RIRI后Fas/FasL表達水平升高,而玻璃體腔內(nèi)注射BMSC抑制Fas/FasL的表達,證明BMSC移植對RIRI有治療作用。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of Fas and FasL, the apoptosis-related factors of retinal ischemia reperfusion injury in rats with retinal ischemia-reperfusion injury. Methods BMSCs were cultured by adherent screening in vitro. Adult SD rats were randomly divided into normal group (n = 3) and control group (n = 3). There were 18 rats in the model group and 18 rats in the BMSC transplantation group. All the rats in each group were selected as experimental eyes to establish RIRI model. The other two groups were given intravitreous injection of PBS buffer solution 1 h after RIRI (model group), and 3 SD rats were randomly killed at 2 h, 6 h, 12 h, 24 h, 48 h and 72 h after RIRI, respectively. The number of Fas and FasL positive cells in rat retina was detected by immunohistochemical method. Results the low expression of Fas in the whole retinal layer of normal retina was rarely detected. 2 h after 2 h, 12 h, 12 h, 24 h and 48 h and 72 h after RIRI, the model was established. The number of Fas positive cells in the transplantation group was 263.6 鹵15.6 鹵263.6 鹵15.6) 路m ~ (-2) 路m ~ (-2), respectively, 1184.6 鹵45. 5 鹵45. 5 鹵45. 5) 路m ~ (-2) and 684.5 鹵3. 5 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) of Fas positive cells in the transplantation group were 19.8 鹵0. 8 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 鹵7 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-1)??? The number of FasL positive cells in the BMSC transplantation group was significantly lower than that in the BMSC transplantation group at 648 h after 648 h. The number of FasL positive cells was 55.6 鹵0.7. The number of FasL was 351.2 鹵6.8. the number of FasL was significantly lower in BMSC transplantation group than that in BMSC transplantation group (P 0.05) at 1248 h. There was a significant difference in the number of Fas-FasL positive cells between the two groups at 1248 h (P 0.05) and P 0.05 (P 0.05) at 1248 h. The number of Fas-FasL positive cells was significantly lower in BMSC transplantation group than that in the model group at each time point (P < 0.05). The number of FasL positive cells in BMSC transplantation group was significantly lower than that in BMSC transplantation group at 648 h. There were significant differences in the number of Fas-FasL positive cells between two groups (P 0.05) at 1248 h. Conclusion the expression of Fas/FasL increased after RIRI. Intravitreal injection of BMSC inhibits the expression of Fas/FasL, indicating that BMSC transplantation has therapeutic effect on RIRI.
【作者單位】： 濰坊醫(yī)學院眼科教研室;濰坊醫(yī)學院附屬醫(yī)院眼科中心;山東省魯南眼科醫(yī)院;
【基金】：山東省自然科學基金資助(編號:Y2007C084) 山東省教育廳基金資助(編號:J07YE02)~~
【分類號】：R774.1

【參考文獻】

相關期刊論文 前2條

1 魯玲玲,劉玉軍,孫曉紅,劉華松,孫正,楊慧;骨髓間充質(zhì)干細胞在大鼠體內(nèi)的遷移研究[J];中國生物工程雜志;2005年04期

2 牛膺筠,張瑞,周占宇,王紅云,劉夫玲;堿性成纖維細胞生長因子對鼠視網(wǎng)膜缺血再灌注損傷的治療作用[J];中華眼科雜志;2002年09期

【共引文獻】

相關期刊論文 前10條

1 袁春燕;牛膺筠;張瑞;丁玉枝;;視網(wǎng)膜缺血再灌注損傷后凋亡相關基因表達的變化[J];青島大學醫(yī)學院學報;2006年04期

2 陳海;孫善全;汪克建;冉建華;余維華;楊美;;水通道蛋白-4在急性高眼壓大鼠眼組織的表達變化[J];重慶醫(yī)學;2006年01期

3 馬艷萍;朱承華;杭薈;;倍他洛爾與氨基胍對大鼠視網(wǎng)膜缺血再灌注損傷的保護作用[J];國際眼科雜志;2008年02期

4 石芊;彭秀軍;;干細胞移植治療視網(wǎng)膜疾病的研究現(xiàn)狀[J];國際眼科雜志;2008年05期

5 徐巍;徐國興;;間充質(zhì)干細胞在視網(wǎng)膜疾病研究中的應用[J];國際眼科雜志;2011年02期

6 高峰麗;戚雪;;黃芪對大鼠視網(wǎng)膜缺血再灌注損傷中Bcl-2和Bax表達的影響[J];國際眼科雜志;2012年05期

7 謝燕丹;劉元生;;骨髓間充質(zhì)干細胞在骨髓移植中的應用[J];廣西醫(yī)學;2007年02期

8 ;Mesenchymal stem cells for retinal diseases[J];International Journal of Ophthalmology(English Edition);2011年04期

9 洪玉;徐國興;;骨髓間充質(zhì)干細胞與視網(wǎng)膜色素上皮細胞的蛋白質(zhì)組學比較[J];福建醫(yī)科大學學報;2012年06期

10 王鳳武;郝斐;魏著英;劉東軍;榮威恒;;綿羊羊水干細胞外源基因高效轉(zhuǎn)染及快速篩選方法的建立[J];中國畜牧獸醫(yī);2013年11期

相關會議論文 前1條

1 孟根花;李浩軍;烏仁圖雅;;珍珠丸對兔視網(wǎng)膜缺血再灌注的神經(jīng)保護作用及Bcl-2基因的表達[A];內(nèi)蒙古醫(yī)學院中蒙醫(yī)學院50周年校慶論文專輯（蒙醫(yī)蒙藥）[C];2006年

相關博士學位論文 前10條

1 洪玉;骨髓間充質(zhì)干細胞體外誘導分化為光感受器樣細胞的蛋白質(zhì)組學研究[D];福建醫(yī)科大學;2011年

2 曹陽;SDF-1/CXCR4對大鼠骨髓基質(zhì)細胞遷移的影響及在脊髓損傷中的應用[D];中國醫(yī)科大學;2006年

3 宋可新;黑色素細胞與骨髓間充質(zhì)干細胞復合構建組織工程皮膚的初步研究[D];中國協(xié)和醫(yī)科大學;2008年

4 晁煒靜;人脂肪間充質(zhì)干細胞分化為視網(wǎng)膜細胞及對視網(wǎng)膜變性疾病治療的實驗研究[D];中國協(xié)和醫(yī)科大學;2008年

5 楊治坤;脂肪來源的間充質(zhì)干細胞在糖尿病視網(wǎng)膜病變大鼠模型中分化和功能的初步研究[D];中國協(xié)和醫(yī)科大學;2008年

6 陳，

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