發(fā)布時(shí)間：2018-02-26 20:14

  本文關(guān)鍵詞： 白介素-18 視網(wǎng)膜新生血管 血管內(nèi)皮生長(zhǎng)因子　出處：《石河子大學(xué)》2011年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的：探討白介素-18對(duì)大鼠視網(wǎng)膜中血管內(nèi)皮生長(zhǎng)因子(VEGF)及新生血管形成的影響。 方法：采用同日出生7天的SD大鼠幼鼠50只,共100只眼,隨機(jī)選取40只統(tǒng)一編號(hào)后置于750±20mL/L氧倉(cāng)中連續(xù)飼養(yǎng)5d,建立高氧誘導(dǎo)的血管增生性視網(wǎng)膜病變模型。剩余10只為正常對(duì)照組,不給予任何處理,正常環(huán)境中飼養(yǎng)。建模后幼鼠隨機(jī)分為試驗(yàn)低濃度組(10只)：給予雙眼玻璃體內(nèi)注射1ul的IL-18(10ng/u1)。實(shí)驗(yàn)高濃度組(10只)：給予雙眼玻璃體內(nèi)注射1ul的IL-18(50ng/u1)。生理鹽水組(10只)：給予雙眼玻璃體內(nèi)注射lul的生理鹽水。高氧對(duì)照組(10只)：雙眼不給予任何處理。于第21d將五組小鼠頸椎脫臼處死,取新鮮視網(wǎng)膜組織進(jìn)行HE染色組織學(xué)切片上觀察并計(jì)數(shù)突破視網(wǎng)膜內(nèi)界膜進(jìn)入玻璃體的新生血管內(nèi)皮細(xì)胞核數(shù)目、免疫組織化學(xué)法檢測(cè)VEGF的表達(dá),CD34標(biāo)記血管內(nèi)皮細(xì)胞觀察視網(wǎng)膜血管改變,比較各組間差異。 結(jié)果：①HE染色顯示,在組織切片上可見(jiàn)正常對(duì)照組(1.40±0.70)內(nèi)界膜清晰、連續(xù),極少見(jiàn)新生血管內(nèi)皮細(xì)胞核。而其他組均有較多新生血管內(nèi)皮細(xì)胞核突破視網(wǎng)膜內(nèi)界膜進(jìn)入玻璃體腔內(nèi),但實(shí)驗(yàn)組明顯低于生理鹽水組(14.9±1.12)及高氧對(duì)照組(14.7±2.26),并且在用高濃度實(shí)驗(yàn)組(6.50±1.27)少于低濃度實(shí)驗(yàn)組(10.1±1.52)。②正常對(duì)照組(0.30±0.48)極少見(jiàn)VEGF陽(yáng)性表達(dá),實(shí)驗(yàn)組、生理鹽水組及高氧對(duì)照組均發(fā)現(xiàn)較多表達(dá)VEGF的陽(yáng)性細(xì)胞,在生理鹽水組(3.70±0.48)及高氧對(duì)照組(3.50±0.71)表達(dá)最明顯,而實(shí)驗(yàn)組VEGF陽(yáng)性表達(dá)明顯低于生理鹽水組及高氧對(duì)照組(P0.05),且低濃度實(shí)驗(yàn)組(2.60±0.52)與高濃度實(shí)驗(yàn)組(1.90±0.74)間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。⑧正常對(duì)照組(1.3±0.48)極少見(jiàn)增生的毛細(xì)血管,實(shí)驗(yàn)組、生理鹽水組及高氧對(duì)照組發(fā)現(xiàn)較多CD34標(biāo)記的增生的毛細(xì)血管,在生理鹽水組(6.9±0.11)及高氧對(duì)照組(6.6±0.97)增生最明顯,實(shí)驗(yàn)組相對(duì)較少,且低濃度實(shí)驗(yàn)組(3.8±0.79)與高濃度實(shí)驗(yàn)組(2.8±0.79)間之間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論：IL-18玻璃體腔內(nèi)注射可抑制血管內(nèi)皮細(xì)胞的增殖及遷移,從而抑制視網(wǎng)膜新生血管的形成。
[Abstract]:Aim: to investigate the effects of interleukin-18 (IL-18) on vascular endothelial growth factor (VEGF) and neovascularization in rat retina. Methods: a total of 50 SD rats (100 eyes) were born on the same day. 40 rats were randomly selected and fed in 750 鹵20ml / L oxygen chamber for 5 days to establish hyperoxia-induced angioproliferative retinopathy model. The remaining 10 rats were normal control group without any treatment. After modeling, young rats were randomly divided into low concentration group (n = 10): intravitreous injection of 1ul / L IL-18ng / u ~ (-1), experimental high concentration group (n = 10): intravitreous injection of 1ul / L ~ (50 ng / u ~ (-1)). Normal saline group (n = 10) were given intravitreous injection of 1ul (IL-18g / u ~ (-1)) and normal saline (n = 10). 10 rats in hyperoxia control group were given intravitreous injection of normal saline with lul. The rats in the hyperoxia control group were killed by cervical dislocations on the 21st day without any treatment in both eyes. The number of neovascularized endothelial nuclei entering vitreous body through the membrane of retinal inner boundary was observed and counted on the histological sections of fresh retina tissue by HE staining. Immunohistochemical method was used to detect the expression of VEGF and CD34 labeled vascular endothelial cells (VECs) to observe the changes of retinal blood vessels and to compare the differences among the groups. Results the results showed that the normal control group (1.40 鹵0.70) had a clear and continuous internal boundary membrane. The nucleus of neovascularization endothelial cells was rarely seen in the other groups, while in other groups, the nucleus of neovascularization entered the vitreous cavity through the inner limiting membrane of the retina. However, the positive expression of VEGF in the experimental group was significantly lower than that in the saline group (14.9 鹵1.12) and the hyperoxia control group (14.7 鹵2.26), and the expression of VEGF in the high concentration group (6.50 鹵1.27) was less than that in the low concentration group (10.1 鹵1.52n.2) in the normal control group (0.30 鹵0.48). Positive cells expressing VEGF were found in saline group and hyperoxia control group. The expression of VEGF was most obvious in saline group (3.70 鹵0.48) and hyperoxia control group (3.50 鹵0.71). The positive expression of VEGF in the experimental group was significantly lower than that in the saline group and hyperoxia control group (P 0.05), and there was a significant difference between the low concentration group (2.60 鹵0.52) and the high concentration group (1.90 鹵0.74). In the saline group and hyperoxia control group, more CD34 labeled hyperplastic capillaries were found, especially in the saline group (6.9 鹵0.11) and hyperoxia control group (6.6 鹵0.97). There was significant difference between the low concentration group (3.8 鹵0.79) and the high concentration group (2.8 鹵0.79). Conclusion Intravitreous injection of% IL-18 can inhibit the proliferation and migration of vascular endothelial cells and thus retina neovascularization.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類(lèi)號(hào)】：R774.1

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

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