本文關(guān)鍵詞： microRNA 鼻咽癌 腫瘤 腫瘤分期　出處：《廣西醫(yī)科大學(xué)》2011年碩士論文　論文類型：學(xué)位論文

【摘要】：目的檢測鼻咽非角化型癌患者血清中微小RNA-29c(microRNA-29c,miR-29c)的表達(dá)量,探討miR-29c與鼻咽非角化型癌發(fā)生、發(fā)展和侵襲轉(zhuǎn)移的關(guān)系。 方法鼻咽癌組為24例鼻咽非角化型癌患者,正常組為24例健康人,收集兩組的血清,用Q-PCR方法檢測血清中miR-29c的表達(dá)量。 結(jié)果(1)鼻咽非角化型癌患者血清中miR-29c的表達(dá)量明顯低于健康人血清中miR-29c的表達(dá)量(P0.05);(2)有淋巴結(jié)轉(zhuǎn)移的鼻咽非角化型癌患者血清中miR-29c的表達(dá)量明顯低于無淋巴結(jié)轉(zhuǎn)移的鼻咽非角化型癌患者血清中miR-29c的表達(dá)量(P0.05);(3)鼻咽癌2008分期Ⅳ期的鼻咽非角化型癌患者血清中miR-29c的表達(dá)量明顯低于Ⅱ-Ⅲ期的鼻咽非角化型癌患者血清中miR-29c的表達(dá)量(P0.05);(4)不同性別的鼻咽非角化型癌患者血清中miR-29c的表達(dá)量無明顯差異(P0.05);(5)不同年齡的鼻咽非角化型癌患者血清中miR-29c的表達(dá)量無明顯差異(P0.05)。 結(jié)論鼻咽非角化型癌患者血清中miR-29c表達(dá)量明顯降低,有淋巴結(jié)轉(zhuǎn)移和臨床分期晚的患者血清中miR-29c的表達(dá)量更低。提示miR-29c可能與鼻咽癌的發(fā)生、發(fā)展和侵襲轉(zhuǎn)移有關(guān)。
[Abstract]:Objective to detect the expression of microRNA-29cRNA-29cmmiR-29c in the serum of patients with nonkeratinized nasopharyngeal carcinoma, and to investigate the relationship between miR-29c and the occurrence, development, invasion and metastasis of nasopharyngeal non-keratinized carcinoma. Methods Twenty four patients with nasopharyngeal non-keratinized carcinoma and 24 normal controls were enrolled in this study. Serum samples from two groups were collected and the expression of miR-29c was detected by Q-PCR. Results (1) the expression of miR-29c in serum of patients with nonkeratinized nasopharynx carcinoma was significantly lower than that of healthy persons (P 0.05). The expression of miR-29c in patients with non-keratinized nasopharyngeal carcinoma with lymph node metastasis was significantly lower than that in patients without lymph node transformation. Expression of miR-29c in serum of patients with non-keratinized carcinoma of nasopharynx (P0.05P0.05) the expression of miR-29c in patients with stage 鈪，

本文編號：1537017