本文關(guān)鍵詞： 鼻息肉 FOXP3 T淋巴細(xì)胞亞群 CD4~+T細(xì)胞 CD8~+T細(xì)胞　出處：《鄭州大學(xué)》2010年碩士論文　論文類型：學(xué)位論文

【摘要】： 背景和目的 鼻息肉(nasal polyps, NP)是鼻腔和鼻竇粘膜的慢性炎癥性疾病,發(fā)病率約占總?cè)藬?shù)的1%～4%。臨床上以慢性鼻-鼻竇炎伴鼻息肉(CRSwNP)較為多見,其復(fù)發(fā)率較高。發(fā)病機(jī)制目前尚不清楚,近年來的研究表明可能與感染、變態(tài)反應(yīng)、免疫及細(xì)胞因子等有關(guān)。 叉頭狀轉(zhuǎn)錄因子3(forkhead transcription factor p3, Foxp3)是一個(gè)新發(fā)現(xiàn)的轉(zhuǎn)錄調(diào)節(jié)因子,對CD4+ CD25+ T調(diào)節(jié)性細(xì)胞的發(fā)育及功能的維持至關(guān)重要,并具有免疫抑制作用。FOXP3已被證明在CD4+ CD25+ T調(diào)節(jié)性細(xì)胞上特異性表達(dá),在一定程度上反映CD4+ CD25+ T調(diào)節(jié)性細(xì)胞的水平和功能活性。 本研究旨在分析FOXP3、CD4和CD8在鼻息肉中的表達(dá)及分布、弄清FOXP3、與CD4和CD8的相互關(guān)系,探討FOXP3+ CD4+ CD25+ T調(diào)節(jié)細(xì)胞、T淋巴細(xì)胞亞群在鼻息肉發(fā)生、發(fā)展中的作用,明確其臨床意義。 材料與方法 本研究對實(shí)驗(yàn)組50例鼻息肉組織和對照組20例正常下鼻甲黏膜組織的標(biāo)本切片分別行HE染色,采用免疫組化SP法檢測兩組標(biāo)本中FOXP3、CD4以及CD8的表達(dá)情況,采用SPSS16.0統(tǒng)計(jì)軟件,選擇正確的檢驗(yàn)方法,進(jìn)行數(shù)據(jù)分析及對照研究。 結(jié)果 1 FOXP3蛋白的陽性產(chǎn)物主要定位于細(xì)胞核和(或)細(xì)胞質(zhì),呈棕黃色或棕褐色顆粒。免疫組化結(jié)果光鏡下見：鼻息肉組和正常下鼻甲黏膜組均能觀察到FOXP3陽性細(xì)胞。 2 CD4和CD8蛋白陽性產(chǎn)物主要定位于細(xì)胞膜,呈棕黃色或棕褐色顆粒。在鼻息肉組織中見大量的CD4和CD8陽性表達(dá)的細(xì)胞,呈叢集性分布,多集中在上皮下和腺體周圍,CD4+T細(xì)胞數(shù)顯著高于CD8+T細(xì)胞數(shù)。正常下鼻甲黏膜組織中大部分切片也可見少量的CD4+T細(xì)胞和CD8+T細(xì)胞。 3計(jì)算機(jī)圖像分析系統(tǒng)檢測FOXP3、CD4、CD8平均灰度值 (1)FOXP3在50例鼻息肉組織和20例正常下鼻甲黏膜組織中表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。在單側(cè)和雙側(cè)鼻息肉組織中FOXP3表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。FOXP3在鼻息肉首發(fā)組與復(fù)發(fā)組表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。 (2)CD4在50例鼻息肉組織和20例正常下鼻甲黏膜組織中表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.01)。在單側(cè)與雙側(cè)鼻息肉組織中CD4表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。CD4在鼻息肉首發(fā)組與復(fù)發(fā)組表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。 (3)CD8在50例鼻息肉組織和20例正常下鼻甲粘膜組織中表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。在單側(cè)與雙側(cè)鼻息肉組織中CD8表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。CD8在鼻息肉首發(fā)組與復(fù)發(fā)組表達(dá)的平均灰度值差異具有統(tǒng)計(jì)學(xué)意義(p0.05)。 4通過spearson相關(guān)分析,在鼻息肉組織中CD4與FOXP3之間存在負(fù)相關(guān)關(guān)系(γ=-0.383,p0.01)；CD8與FOXP3之間存在負(fù)相關(guān)關(guān)系(r=-0.364,p0.01)。 結(jié)論 1FOXP3在鼻息肉組織中表達(dá)下調(diào),提示FOXP3的缺乏或功能低下在鼻息肉的發(fā)生和發(fā)展中起著重要的作用。 2FOXP3、CD4和CD8均參與鼻息肉的形成過程,且可能鼻息肉的復(fù)發(fā)特性有關(guān)。 3FOXP3、CD4和CD8在鼻息肉中的表達(dá)具有相關(guān)性,提示三種蛋白可能協(xié)同參與鼻息肉的形成過程。 4檢測鼻息肉組織中FOXP3表達(dá),可以了解鼻息肉微環(huán)境中CD4+ CD25+調(diào)節(jié)性T細(xì)胞浸潤狀態(tài),從而可判定鼻息肉組織局部免疫反應(yīng)狀態(tài),為鼻息肉提供新的治療方法。
[Abstract]:Background and purpose
Nasal polyps (nasal polyps NP) is a chronic inflammatory disease of nasal cavity and paranasal sinus mucosa, the incidence rate of about 1% of the total number of ~ 4%. in clinical chronic nasal sinusitis with nasal polyps (CRSwNP) is more common, the high recurrence rate. The pathogenesis is still unclear. Recent studies have shown that abnormal may react with infection, immunity and cytokines and so on.
Forkhead transcription factor 3 (forkhead transcription factor P3, Foxp3) is a newly discovered transcription factor, regulating vital to maintain the development and function of cells of CD4+ CD25+ T, and has the immunosuppressive effect of.FOXP3 has been shown to regulate the expression of cell specific CD4+ CD25+ in T, and reflect the level of functional activity of CD4+ CD25+ T regulatory cells in a certain extent.
The purpose of this study is to analyze the expression and distribution of FOXP3, CD4 and CD8 in nasal polyps, clarify the relationship between FOXP3 and CD4 and CD8, and to explore the role of FOXP3+ CD4+ CD25+ T regulating cells and the subsets of T lymphocytes in the occurrence and development of nasal polyps, and to clarify their clinical significance.
Materials and methods
In this study, the experimental group 50 cases of nasal polyps and 20 cases of control group specimens under normal turbinate mucosa were stained with HE, were detected by immunohistochemistry SP method in two groups. FOXP3, CD4 and CD8 expression, using SPSS16.0 statistical software, choose the correct test method of data analysis and the control.
Result
1, the positive products of FOXP3 protein were mainly located in nuclei and / or cytoplasm. They were brown or brown. Immunohistochemistry showed that FOXP3 positive cells could be observed in nasal polyps and normal inferior turbinate mucosa.
2 CD4 and CD8 protein was mainly expressed in the cell membrane, brownish yellow or brown particles. See CD4 and CD8 positive expression of a large number of cells in nasal polyps, a cluster distribution, mostly concentrated in the surrounding epithelium and gland, CD4+T cell number was significantly higher than that of CD8+T cells in normal mucosa tissues. In most sections of a small amount of CD4+T cells and CD8+T cells.
3 computer image analysis system to detect the average gray value of FOXP3, CD4, CD8
(1) the average gray level expression of FOXP3 in 50 cases of nasal polyps and 20 cases of normal mucosa in the value of the difference was statistically significant (P0.05). In the unilateral and bilateral nasal polyps in the average gray value of FOXP3 expression difference was statistically significant (P0.05.FOXP3) in the first group and the average gray nasal polyp recurrence group the expression of the value of the difference was statistically significant (P0.05).
(2) the average gray level expression of CD4 in 50 cases of nasal polyps and 20 cases of normal mucosa in the value of the difference was statistically significant (P0.01). In the unilateral and bilateral nasal polyps in the average gray value of CD4 expression difference was statistically significant (P0.05) the average gray.CD4 in the first group and the recurrence of nasal polyps expression of the value of the difference was statistically significant (P0.05).
(3) the average gray level expression of CD8 in 50 cases of nasal polyps and 20 cases of normal mucosa in the value of the difference was statistically significant (P0.05). In the unilateral and bilateral nasal polyps in the average gray value of CD8 expression difference was statistically significant (P0.05) the average gray.CD8 in the first group and the recurrence of nasal polyps expression of the value of the difference was statistically significant (P0.05).
4, through spearson correlation analysis, there was a negative correlation between CD4 and FOXP3 (=-0.383, P0.01) in nasal polyps, and there was a negative correlation between CD8 and FOXP3 (r=-0.364, P0.01).
conclusion
The expression of 1FOXP3 is downregulated in the nasal polyps, suggesting that the lack or infunction of FOXP3 plays an important role in the occurrence and development of nasal polyps.
2FOXP3, CD4 and CD8 are involved in the formation of nasal polyps, and may be related to the recurrent characteristics of nasal polyps.
The expression of 3FOXP3, CD4 and CD8 in nasal polyps is related, suggesting that the three proteins may be involved in the formation of nasal polyps.
4 detecting the expression of FOXP3 in nasal polyps, we can understand the infiltration state of CD4+ CD25+ regulatory T cells in the nasal polyp microenvironment, so that we can determine the local immune response state of nasal polyps, and provide a new treatment for nasal polyps.

【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R765.25

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