發(fā)布時(shí)間：2018-02-22 19:18

  本文關(guān)鍵詞： 貝伐單抗 血管纖維化 炎癥因子 人臍靜脈內(nèi)皮細(xì)胞　出處：《眼科新進(jìn)展》2015年11期 　論文類型：期刊論文

【摘要】：目的觀察貝伐單抗對(duì)人臍靜脈內(nèi)皮細(xì)胞(human umbilical vein endothelial cells,HUVEC)纖維化相關(guān)炎癥因子表達(dá)的影響,以探討貝伐單抗治療后新生血管纖維化的可能機(jī)制。方法分別在正常氧含量及缺氧條件下培養(yǎng)HUVEC,培養(yǎng)液中加入終濃度為0.25g·L-1貝伐單抗,根據(jù)處理時(shí)間的不同分為0 h組(對(duì)照組)、6 h組、12 h組、24 h組、48 h組。分別用RT-PCR及ELISA方法檢測(cè)各組纖維化相關(guān)炎癥因子白細(xì)胞介素(interleukin,IL)-1β、IL-6、IL-8以及腫瘤壞死因子-α(tumor necrosis factor-alpha,TNF-α)mRNA和蛋白的表達(dá)。結(jié)果正常氧含量條件下,與對(duì)照組相比,IL-6、IL-8的mRNA和蛋白表達(dá)在各實(shí)驗(yàn)組均明顯增加,而IL-1β、TNF-αmRNA和蛋白的表達(dá)僅在12 h、24 h、48 h組明顯增加,差異均有統(tǒng)計(jì)學(xué)意義(均為P0.05)。缺氧條件下,與對(duì)照組相比各實(shí)驗(yàn)組IL-1β、IL-6、IL-8及TNF-α的mRNA和蛋白表達(dá)在各時(shí)間點(diǎn)均明顯增加,差異均有統(tǒng)計(jì)學(xué)意義(均為P0.05)。結(jié)論無論在正常氧含量還是缺氧條件下,貝伐單抗均可刺激纖維化相關(guān)炎癥因子的表達(dá)增加,此部分炎癥因子可能參與了抗新生血管內(nèi)皮生長(zhǎng)因子治療后的新生血管纖維化過程。
[Abstract]:Objective to observe the effect of bevacizumab on the expression of inflammatory cytokines associated with fibrosis in human umbilical vein endothelial cells (HUVECs) of human umbilical vein endothelial cells (HUVECs). Methods HUVECs were cultured under the condition of normal oxygen content and hypoxia respectively, and the final concentration of bevacizumab was 0.25 g 路L ~ (-1). According to the time of treatment, they were divided into 0 h group (control group, 6 h group, 12 h group, 24 h group, 48 h group, respectively). RT-PCR and ELISA methods were used to detect interleukin-1 尾 -IL-6 IL-8, tumor necrosis factor- 偽 tumor necrosis factor- 偽 mRNA and TNF- 偽 mRNA, respectively. Results under the condition of normal oxygen content, Compared with the control group, the expression of mRNA and protein of IL-8 was significantly increased in all experimental groups, while the expression of TNF- 偽 mRNA and protein in IL-1 尾 -TNF- 偽 increased only at 12 h and 24 h after 48 h, the difference was statistically significant (all P0.050.The expression of TNF- 偽 mRNA and protein in hypoxia group was significantly higher than that in control group. Compared with the control group, the expression of mRNA and protein of IL-1 尾 -IL-6, IL-8 and TNF- 偽 in each experimental group were significantly increased at each time point, and the difference was statistically significant (P 0.05). Conclusion the expression of IL-8 and TNF- 偽 in the experimental group is significantly higher than that in the control group (P 0.05). Bevacizumab can stimulate the expression of fibrosis-related inflammatory factors, which may be involved in the process of neovascularization after anti-angiogenic endothelial growth factor treatment.
【作者單位】： 青島大學(xué)醫(yī)學(xué)院 山東省眼科研究所;山東省眼科研究所 青島眼科醫(yī)院;
【基金】：山東省自然科學(xué)基金資助(編號(hào):ZR2014HM029) 青島市科技計(jì)劃項(xiàng)目(編號(hào):13-1-3-69-nsh)~~
【分類號(hào)】：R773.4

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本文編號(hào)：1525101