本文關(guān)鍵詞： 鼻炎 變應(yīng)性 樹突狀細(xì)胞　出處：《臨床耳鼻咽喉頭頸外科雜志》2014年13期 　論文類型：期刊論文

【摘要】：目的:研究鼻敏感顆粒對變應(yīng)性鼻炎小鼠樹突狀細(xì)胞(DC)發(fā)揮免疫調(diào)節(jié)作用的具體機(jī)制。方法:分離出小鼠骨髓細(xì)胞,使用rGM-CSF和rIL-4共培養(yǎng)骨髓細(xì)胞,刺激骨髓細(xì)胞向DC轉(zhuǎn)化。然后加入脂多糖和不同濃度的鼻敏感顆粒共刺激未成熟DC,12h后檢測DC中MHCⅡ、CD80、CD86表面分子以及細(xì)胞上清中TNF-α、IL-1β、IL-6和IL-10的表達(dá)。結(jié)果:鼻敏感顆粒能夠顯著抑制成熟DC標(biāo)志性細(xì)胞因子IL-6、TNF-α、IL-10、IL-1β的生成和成熟DC標(biāo)志物MHCⅡ、CD80和CD86的表達(dá)。結(jié)論:鼻敏感顆粒能夠顯著抑制未成熟DC向成熟DC的分化。
[Abstract]:Objective: to study the immunomodulatory mechanism of Bi-sensitive granules on dendritic cells of allergic rhinitis mice. Methods: bone marrow cells were isolated from mice and co-cultured with rGM-CSF and rIL-4. Bone marrow cells were stimulated to transform into DC, then lipopolysaccharide and nasal sensitive granules were added to stimulate immature DC for 12h. After 12 hours, the surface molecules of MHC 鈪，

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