本文關(guān)鍵詞： 眼眶 橫紋肌肉瘤 legumain α-SMA TGF-β RD　出處：《天津醫(yī)科大學(xué)》2010年博士論文　論文類型：學(xué)位論文

【摘要】： 目的 1檢測人眼眶橫紋肌肉瘤(rhabdomyosarcoma,RMS)組織中l(wèi)egumain的表達,觀察legumain的表達與RMS生物學(xué)行為之間的關(guān)系。并對橫紋肌肉瘤患者的多個臨床指標與患者預(yù)后進行多因素分析,對影響生存率的獨立因素進行分析討論。 2檢測經(jīng)不同濃度的轉(zhuǎn)化生長因子β(transforming growth factorβ,TGF-β)刺激之后人胚胎型RMS細胞系RD細胞中l(wèi)egumain及α-平滑肌肌動蛋白(α-smooth muscle actin,α-SMA)表達的變化,探討legumain在RD細胞中的作用,為腫瘤的治療提供新的思路和策略。 方法 1應(yīng)用免疫組化SP法檢測56例人眼眶RMS患者手術(shù)標本中l(wèi)egumain的表達,并引入年齡、性別、組織學(xué)類型、腫瘤大小、是否累及眼瞼等因素進行統(tǒng)計學(xué)分析,觀察各因素與患者預(yù)后的關(guān)系。 2首先利用細胞免疫熒光法檢測1egumain在RD細胞中的表達情況,并使用不同濃度的TGF-β對RD細胞進行刺激,通過RT-PCR以及Western-blot技術(shù)檢測刺激前后legumain及α-SMA在RD細胞中表達的變化。 結(jié)果 1 legumain在56例RMS組織中有25例高表達,并且其表達高低、腫瘤大小、組織學(xué)類型、是否累及眼瞼等因素是影響橫紋肌肉瘤患者生存率的獨立臨床因素。 2通過細胞免疫熒光法檢測到RD細胞中存在legumain的表達,并且legumain及α-SMA的表達在2.5ng／ml的TGF-β刺激后較正常對照組明顯升高,而5ng／ml刺激后RD細胞中l(wèi)egumain及α-SMA的表達較正常對照組明顯降低。 結(jié)論 1 legumain在RMS組織中具有較高的表達,檢測人眼眶RMS組織中l(wèi)egumain的表達有望成為判斷腫瘤惡性程度以及患者預(yù)后的生物學(xué)指標,并為以后臨床治療眼眶RMS提供實驗基礎(chǔ)。 2 Legumian低表達、腫瘤"f5cm、胚胎型RMS、腫瘤未累及眼瞼的病人均具有較高生存率。而患者的年齡、性別因素與患者的預(yù)后無關(guān)。 3低濃度的TGF-p刺激提高了RD細胞中l(wèi)egumain及α-SMA的表達,而高濃度的TGF-β刺激降低了RD細胞中l(wèi)egumain及α-SMA的表達,由于低濃度的TGF-β可以促進RD細胞的生長,而高濃度的TGF-β可以通過某種途徑抑制RD細胞的生長,因此,legumain可能通過某種途徑參與了TGF-β調(diào)控RD細胞生長的環(huán)節(jié),從而增加了腫瘤細胞的侵襲性。
[Abstract]:Purpose 1the expression of legumain in human orbital rhabdomyosarcoma sarcoma (RMS) was detected. To observe the relationship between the expression of legumain and the biological behavior of RMS, and to analyze the multiple clinical indexes and prognosis of patients with rhabdomyosarcoma. The independent factors affecting survival rate were analyzed and discussed. 2TGF- 尾 -transforming growth factor 尾 was detected at different concentrations. TGF- 尾 stimulated legumain and 偽 -smooth muscle actin in human embryonic RMS cell line Rd. To explore the role of legumain in Rd cells and to provide new ideas and strategies for the treatment of tumors. Method 1 Immunohistochemical SP method was used to detect the expression of legumain in 56 patients with orbital RMS. Age, sex, histological type and tumor size were introduced. Whether the eyelid was involved or not was analyzed statistically to observe the relationship between the factors and the prognosis of the patients. 2Firstly, the expression of 1egumain in Rd cells was detected by cell immunofluorescence assay, and different concentrations of TGF- 尾 were used to stimulate Rd cells. The expression of legumain and 偽 -SMA in Rd cells was detected by RT-PCR and Western-blot techniques before and after stimulation. Results 1 there were 25 cases of high expression of legumain in 56 cases of RMS, and its expression level, tumor size, histological type. Whether the eyelid is involved or not is an independent clinical factor affecting the survival rate of patients with rhabdomyosarcoma. 2the expression of legumain in Rd cells was detected by immunofluorescence assay. The expression of legumain and 偽 -SMA was significantly higher than that of the control group after stimulation with 2.5 ng / ml TGF- 尾. The expression of legumain and 偽 -SMA in Rd cells after 5 ng / ml stimulation was significantly lower than that in normal control group. Conclusion 1 legumain was highly expressed in RMS. Detection of the expression of legumain in human orbital RMS is expected to be a biological index to judge the malignancy and prognosis of the tumor and to provide experimental basis for clinical treatment of orbital RMS. 2 low expression of Legumian, tumor. The survival rate was higher in patients with F5 cm, embryonic RMS, tumor without eyelid involvement, but age and sex factors were not associated with prognosis. (3) low concentration of TGF-p increased the expression of legumain and 偽 -SMA in Rd cells. The high concentration of TGF- 尾 decreased the expression of legumain and 偽 -SMA in Rd cells, and the low concentration of TGF- 尾 could promote the growth of Rd cells. However, high concentration of TGF- 尾 can inhibit the growth of Rd cells through some way, so legumain may participate in the regulation of the growth of Rd cells through some way. This increases the invasiveness of tumor cells.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2010
【分類號】：R739.7

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本文編號：1461177