第一部分注射用前列地爾對(duì)大鼠心肌缺血/再灌注損傷的影響目的:觀察注射用前列地爾對(duì)大鼠心肌缺血/再灌注損傷的影響,并初步探討其作用機(jī)制。方法:（1）選用健康雄性SD大鼠,隨機(jī)分為4組:假手術(shù)組（Sham組）,心肌缺血/再灌注模型組（I/R組）,前列地爾低劑量組（Alprostadil 4μg/kg）,前列地爾高劑量組（Alprostadil 8μg/kg）。（2）結(jié)扎大鼠左冠狀動(dòng)脈前降支使其缺血30min,再灌注24h,制備心肌缺血/再灌注損傷模型。再灌注24h后檢測(cè)血清肌鈣蛋白T（Troponin T）、肌酸激酶同工酶（CK-MB）及乳酸脫氫酶（LDH）水平、NBT法檢測(cè)心肌梗死面積、應(yīng)用超聲心動(dòng)圖檢測(cè)大鼠心功能（再灌注72h）、在光鏡及電鏡下觀察心肌組織形態(tài)學(xué)特點(diǎn)、TUNEL原位標(biāo)記法和DAPI雙重染色檢測(cè)大鼠心肌細(xì)胞凋亡、檢測(cè)心肌組織丙二醛（MDA）含量、超氧化物歧化酶（SOD）及過(guò)氧化氫酶（CAT）活性。結(jié)果:（1）與Sham組相比,模型組血清Troponin T、CK-MB及LDH均增高,差異有統(tǒng)計(jì)學(xué)意義（p<0.05）。與模型組相比,前列地爾4或8μg/kg組血清Tro... 

【文章來(lái)源】：吉林大學(xué)吉林省 211工程院校 985工程院校 教育部直屬院校

【文章頁(yè)數(shù)】：122 頁(yè)

【學(xué)位級(jí)別】：博士

【部分圖文】：

4，電鏡下觀察心肌細(xì)胞，模型組大鼠心肌細(xì)胞細(xì)胞核和線粒體都發(fā)生了嚴(yán)重?fù)p傷，表現(xiàn)為心肌肌節(jié)纖維排列紊亂，肌絲溶解、斷裂、Z線不清；

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