目的探討微小RNA（microRNA,miR）-155對(duì)氧化型低密度脂蛋白（oxidized-LDL particles,oxLDL）活化的RAW264.7細(xì)胞分泌的趨化因子的調(diào)控作用及其對(duì)輔助性T細(xì)胞1（helper T cell 1, Th1）分化的間接調(diào)控作用。方法培養(yǎng)RAW264.7細(xì)胞,向細(xì)胞內(nèi)轉(zhuǎn)染寡聚核苷酸（miR-ctrl、miR-155 mimics、miR-155 inhibitor、miR-155 mimics+siRNA-ctrl和miR-155 mimics+CXCL10 siRNA）,并以此對(duì)細(xì)胞進(jìn)行分組。oxLDL刺激所有細(xì)胞活化,實(shí)時(shí)定量反轉(zhuǎn)錄聚合酶鏈反應(yīng)（quantitative reverse transcription polymerase chain reaction, qRT-PCR）和酶聯(lián)免疫吸附測(cè)定（enzyme-linked immuno sorbent assay, ELISA）方法檢測(cè)趨化因子表達(dá)情況,將上述細(xì)胞與純化并活化的CD4+T細(xì)胞共培養(yǎng)后,流式細(xì)胞術(shù)檢測(cè)Th1細(xì)胞的分化情況。結(jié)果 miR-155促進(jìn)oxLDL活化的RAW264... 

【文章來(lái)源】：國(guó)際免疫學(xué)雜志. 2020,43(06)

【文章頁(yè)數(shù)】：6 頁(yè)


本文編號(hào)：3516000