circ-Sirt1抑制NF-κB核轉(zhuǎn)位的機(jī)制及在血管炎癥中的意義
發(fā)布時(shí)間：2021-11-01 15:59
目的:動脈粥樣硬化和血管退行性病變是導(dǎo)致心血管疾病的主要病因。炎癥反應(yīng)是導(dǎo)致動脈粥樣硬化和血管退行性病的病理基礎(chǔ)。本研究以SIRT1基因外顯子來源的環(huán)狀非編碼RNA circ-Sirt1為研究對象,從整體、細(xì)胞和分子水平,探討circ-Sirt1在血管炎癥應(yīng)答中的作用和分子機(jī)制。方法:利用野生型SD大鼠建立頸動脈損傷狹窄模型,采用實(shí)時(shí)熒光定量PCR和Western blot方法評價(jià)circ-Sirt1與血管炎癥損傷的關(guān)系。利用Rip和RNA pull-down結(jié)合Western blot技術(shù)發(fā)現(xiàn)并驗(yàn)證與circ-Sirt1相互作用的蛋白。從RNA與蛋白質(zhì)互作角度探討circ-Sirt1對NF-κB p65活化及核轉(zhuǎn)位的影響。結(jié)果:1 circ-Sirt1參與VSMCs表型調(diào)節(jié)1.1 RT-PCR篩選人SIRT1基因產(chǎn)生的環(huán)狀RNA通過對環(huán)狀RNA數(shù)據(jù)庫circBase搜索,發(fā)現(xiàn)人SIRT1基因能產(chǎn)生11種環(huán)狀RNA產(chǎn)物,RT-PCR檢測11種環(huán)狀RNA在人VSMCs中的表達(dá)情況。結(jié)果顯示,hascirc0093887（circ-Sirt1）表...
【文章來源】：河北醫(yī)科大學(xué)河北省
【文章頁數(shù)】：91 頁
【學(xué)位級別】：博士
【部分圖文】：
SIRT1基因編碼產(chǎn)生的circRNAsFig.1-1ExpressionpatternofcircRNAisoformsproducefromSIRT1hostgene(A)RT-PCRswereconductedtodetecttheexpressionofcircRNAisoforms
21圖 1-2 circ-sirt1 序列同源性比較Fig.1-2 circ-Sirt1 gene homology comparisoncirc-Sirt1 homology analysis in rat and human genome was conducted usingBasic Local Alignment Search Tool (BLAST).
22圖 1-3 circ-sirt1 參與血管平滑肌細(xì)胞表型轉(zhuǎn)化Fig.1-3 The expression of circ-Sirt1 is associated with phenotypic switching oVSMCs(A) qRT-PCRs were conducted to detect circ-Sirt1 expression in different rtissues. Data are summarized as mean±SEM, n=6.(B) qRT-PCRs were conducted to detect circ-Sirt1 expression in cells frodifferent sources, including HUVECs, HASMCs and RASMCs. Data asummarized as mean±SEM, n=4.(C, D and E) qRT-PCRs were conducted to detect circ-Sirt1 expression
本文編號：3470390
【文章來源】：河北醫(yī)科大學(xué)河北省
【文章頁數(shù)】：91 頁
【學(xué)位級別】：博士
【部分圖文】：
SIRT1基因編碼產(chǎn)生的circRNAsFig.1-1ExpressionpatternofcircRNAisoformsproducefromSIRT1hostgene(A)RT-PCRswereconductedtodetecttheexpressionofcircRNAisoforms
21圖 1-2 circ-sirt1 序列同源性比較Fig.1-2 circ-Sirt1 gene homology comparisoncirc-Sirt1 homology analysis in rat and human genome was conducted usingBasic Local Alignment Search Tool (BLAST).
22圖 1-3 circ-sirt1 參與血管平滑肌細(xì)胞表型轉(zhuǎn)化Fig.1-3 The expression of circ-Sirt1 is associated with phenotypic switching oVSMCs(A) qRT-PCRs were conducted to detect circ-Sirt1 expression in different rtissues. Data are summarized as mean±SEM, n=6.(B) qRT-PCRs were conducted to detect circ-Sirt1 expression in cells frodifferent sources, including HUVECs, HASMCs and RASMCs. Data asummarized as mean±SEM, n=4.(C, D and E) qRT-PCRs were conducted to detect circ-Sirt1 expression
本文編號：3470390
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