目的:觀察芝麻素（Ses）和維生素E（Vit E）聯(lián)用對自發(fā)性高血壓大鼠（SHRs）左心室纖維化的影響,并探討其可能機(jī)制。方法:14周齡雄性SHRs隨機(jī)分為Ses和Vit E聯(lián)用組(160+10 mg·kg^-1·d^-1、Ses組(160 mg·kg^-1·d^-1)、Vit E組(10mg·kg^-1·d^-1)及SHRs組,另設(shè)正常對照WKY組,每組7只,1次/d持續(xù)灌胃給藥10周。采用尾袖法測定大鼠給藥前和給藥10周后的收縮壓。末次給藥后取左心室,計算臟器指數(shù),Masson染色觀察膠原沉積,分析心肌膠原容積分?jǐn)?shù)（CVF）,Western blot觀察Ⅰ型膠原（collagenⅠ）、Ⅲ型膠原（collagenⅢ）、轉(zhuǎn)化生長因子β₁（TGF-β₁）、結(jié)締組織生長因子（CTGF）、基質(zhì)金屬蛋白酶-9（MMP-9）、基質(zhì)金屬蛋白酶抑制劑-1（TIMP-1）、磷酸化蛋白激酶B（p-AKT）、蛋白激酶B（AKT）和磷酸肌醇3激酶... 

【文章來源】：中國臨床藥理學(xué)與治療學(xué). 2020,25(11)CSCD

【文章頁數(shù)】：9 頁

【文章目錄】：
1 MATERIAL AND METHODS
    1.1 Drugs,reagents and instruments
    1.2 Experimental animals
    1.3 Grouping of experimental animals
    1.4 Measurement of systolic blood pressure
    1.5 Measurement of cardiac index (HW/BW)and left ventricular mass index (LVW/HW)
    1.6 MASSON staining and determination of cardiac fibrosis
    1.7 Western blot analysis
    1.8 Statistical analysis
2 RESULTS
    2.1 Effect of sesamin combined with vitamin E on SBP in SHRs
    2.2 Effect of sesamin combined with vitamin E on HW/BW and LVW/HW in SHRs
    2.3 Effect of sesamin combined with vitamin E on CVF in SHRs
    2.4 Effect of sesamin combined with vitamin Eon left ventricular collagen I and collagen III protein in SHRs
    2.5 Effect of sesamin combined with vitamin Eon left ventricular TGF-β1,CTGF,MMP-9 and TIMP-1 protein in SHRs
    2.6 Effect of sesamin combined with vitamin Eon left ventricular p-AKT,AKT and PI3K protein in SHRs
3 DISCUSSION


【參考文獻(xiàn)】：
期刊論文
[1]芝麻素與維生素E聯(lián)用對代謝綜合征大鼠心肌損傷的協(xié)同保護(hù)作用[J]. 楊解人,黃凱,郭莉群,周勇,吳向起,熊克仁,陳國祥.  中藥藥理與臨床. 2009(05)



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