【摘要】：目的探討微小RNA-146b(miR-146b)對(duì)人單核細(xì)胞株(THP-1)細(xì)胞p38分裂素原活化蛋白激酶(p38MAPK)表達(dá)的影響。方法血管緊張素Ⅱ(AngⅡ)刺激THP-1建立細(xì)胞模型,慢病毒感染pre-miR-146b-3p或anti-miR-146b-3p進(jìn)入THP-1細(xì)胞中,檢測(cè)p38MAPK與環(huán)氧化酶2(COX2)表達(dá)的變化。轉(zhuǎn)入p38MAPK siRNA,檢測(cè)p38MAPK與COX2表達(dá)的變化。結(jié)果 AngⅡ刺激THP-1細(xì)胞p38MAPK與COX2表達(dá)升高,pre-miR-146b-3p可以放大這種增高效應(yīng),當(dāng)轉(zhuǎn)染p38MAPK siRNA后,能逆轉(zhuǎn)pre-miR-146b-3p升高COX2的趨勢(shì)(P0.01)。結(jié)論在AngⅡ刺激的THP-1細(xì)胞中,miR-146b可能通過p38MAPK調(diào)控COX2表達(dá)水平,在心血管疾病的診治中發(fā)揮重要作用。
[Abstract]:Objective to investigate the effect of microRNA-146b (miR-146b) on the expression of p38 mitogen-activated protein kinase (p38MAPK) in human monocyte cell line (THP-1). Methods the cell model was established by angiotensin 鈪，

本文編號(hào)：2498458