高鋁暴露地區(qū)再生障礙性貧血患者免疫細(xì)胞功能的變化
發(fā)布時(shí)間：2019-03-08 15:59
【摘要】：目的:研究高鋁暴露地區(qū)再生障礙性貧血(aplastic anemia,AA)患者免疫細(xì)胞功能的變化及其臨床特點(diǎn),分析不同血鋁水平與免疫細(xì)胞和免疫因子的關(guān)系。方法:選取確診AA病例62例,根據(jù)飲用水鋁含量及病例來(lái)源分為2組,飲用水鋁含量300μg/L并長(zhǎng)期居住于鋁工業(yè)地區(qū)為高鋁暴露地區(qū)AA組,共33例;飲用水鋁含量300ug/L并長(zhǎng)期居住于非鋁工業(yè)地區(qū)為非高鋁暴露地區(qū)AA組,共29例,同時(shí)選取高鋁暴露地區(qū)及非高鋁暴露地區(qū)健康人群各30例作為對(duì)照組。采用電感藕合等離子體質(zhì)譜(inductivity coupled plasma mass spectrometry,ICP-MS)方法檢測(cè)血清鋁水平,酶聯(lián)免疫吸附法(enzyme linked immuno sorbent assay,ELISA)法檢測(cè)細(xì)胞因子IL-10、IL-12、IL-17及INF-γ表達(dá)情況,流式細(xì)胞術(shù)(flow cytometry,FCM)檢測(cè)淋巴細(xì)胞亞群比例,同時(shí)收集患者免疫球蛋白、補(bǔ)體C3、C4表達(dá)水平數(shù)據(jù)及臨床資料。分析高鋁暴露地區(qū)AA患者免疫細(xì)胞及免疫因子變化特點(diǎn),探討血清鋁與免疫指標(biāo)的關(guān)系。結(jié)果:1、高鋁地區(qū)人群(高鋁AA及高鋁健康組)血清鋁水平高于非高鋁地區(qū)人群(非高鋁AA及非高鋁健康)(P0.05)。高鋁暴露地區(qū)AA患者血清鋁水平增高,顯著高于非高鋁暴露地區(qū)AA組及健康對(duì)照組(均P0.01)。2、高鋁暴露地區(qū)AA患者的貧血程度、骨髓造血組織減少程度明顯重于非高鋁暴露地區(qū)AA組(均P0.05),但兩組患者的發(fā)病年齡、性別、白細(xì)胞、中性粒細(xì)胞數(shù)目、網(wǎng)織紅細(xì)胞數(shù)量、血小板數(shù)量、骨髓增生程度無(wú)明顯差異。3、高鋁暴露地區(qū)AA患者CD4+T淋巴細(xì)胞比例、CD4+/CD8+高于非高鋁暴露地區(qū)AA組及健康對(duì)照組(均P0.05),CD8+T淋巴細(xì)胞比例低于非高鋁地區(qū)AA組及健康對(duì)照組(均P0.05),CD3+、CD+19、NK細(xì)胞比例兩組比較無(wú)明顯統(tǒng)計(jì)學(xué)差異(均P0.05)。CD4+T、CD8+T淋巴細(xì)胞比例、CD4+/CD8+與血清鋁水平未見(jiàn)相關(guān)性(r=0.169,P=0.067;r=0.072,P=0.438;r=-0.039,P=0.679)。4、高鋁暴露地區(qū)AA患者IL-10、IFN-γ水平高于非高鋁暴露地區(qū)AA組(均P0.05)。IL-17、IL-12水平兩組比較無(wú)統(tǒng)計(jì)學(xué)差異(均P0.05)。血清鋁水平與細(xì)胞因子IL-10(r=0.072,P=0.4290.05)、IFN-γ(r=0.480,P=0.598)間未見(jiàn)相關(guān)性。5、IgG、IgA、IgM、IgE水平在高鋁暴露地區(qū)AA組與非高鋁暴露地區(qū)AA組間的表達(dá)均無(wú)差異(均P0.05)。6、高鋁暴露地區(qū)AA患者補(bǔ)體C4水平顯著低于非高鋁暴露AA組(P0.01),補(bǔ)體C3水平在兩組間比較無(wú)明顯統(tǒng)計(jì)學(xué)差異(P0.05)。血清鋁與補(bǔ)體C4水平(r=0.097,P=0.311)未見(jiàn)相關(guān)性。結(jié)論:高鋁暴露地區(qū)人群血清鋁水平高于非高鋁暴露地區(qū)人群。高鋁暴露可能影響AA患者免疫功能。高鋁暴露地區(qū)AA免疫細(xì)胞功能特點(diǎn)有別于非高鋁暴露地區(qū)AA,以細(xì)胞免疫和免疫因子改變顯著,血清鋁水平與免疫細(xì)胞和免疫因子間未見(jiàn)相關(guān)性。
[Abstract]:Aim: to study the changes of immune cell function and its clinical characteristics in patients with aplastic anemia (aplastic anemia,AA) exposed to high aluminum, and to analyze the relationship between serum aluminum levels and immune cells and immune factors. Methods: 62 cases of AA were selected and divided into two groups according to the aluminum content of drinking water and the source of the cases. The drinking water aluminum content was 300 渭 g / L and lived for a long time in the high aluminum exposed area of AA group (33 cases). Drinking water aluminum content 300ug/L and long-term living in non-aluminum industrial areas are non-high aluminum exposed areas of AA group, a total of 29 cases, and high aluminum exposure areas and non-high aluminum exposure areas 30 healthy people as the control group. The serum aluminum level was measured by inductively coupled plasma mass spectrometry (inductivity coupled plasma mass spectrometry,ICP-MS), and the expression of cytokines IL-10,IL-12,IL-17 and INF- 緯 was detected by enzyme-linked immunosorbent assay (enzyme linked immuno sorbent assay,ELISA). Flow cytometry (flow cytometry,FCM) was used to detect the proportion of lymphocyte subsets. The data of immunoglobulin, complement C3, C4 expression and clinical data were collected. To analyze the changes of immune cells and immune factors in AA patients exposed to high aluminum, and to explore the relationship between serum aluminum and immune indexes. Results: 1. The level of serum aluminum in high aluminum area (high aluminum AA and high aluminum healthy group) was higher than that in non high aluminum area population (non high aluminum AA and non high aluminum health group) (P0.05). The level of serum aluminum in AA patients in high aluminum exposure area was significantly higher than that in non high aluminum exposed area AA group and healthy control group (P0.01). 2. The anemia degree of AA patients in high aluminum exposed area was significantly higher than that in non high aluminum exposed area (P < 0 01). The degree of bone marrow hemopoietic tissue decrease was more severe than that in AA group (P0.05), but the age, sex, leukocyte, neutrophil, reticulocyte and platelet count in the two groups were significantly higher than those in the control group (P0.05). There was no significant difference in myeloproliferative degree. 3. The ratio of CD4 T lymphocytes and CD4 / CD8 in AA patients exposed to high aluminum were higher than those in AA group and healthy control group in non-aluminum exposed area (P 0.05). The percentage of CD8 T lymphocyte was lower than that of AA group and healthy control group (all P0.05), and there was no significant difference in CD3, CD 19, NK cell ratio between the two groups (P0.05). CD4 T, CD8 T lymphocyte ratio was not significantly different between the two groups (P 0.05). There was no correlation between CD4 / CD8 and serum aluminum level (r = 0.169, P = 0.067). 0. 072, 0. 438, 0. 072, 0. 438; (4) the level of IL-10,IFN- 緯 in AA patients exposed to high aluminum was higher than that in AA patients without high aluminum exposure (P 0.05). There was no significant difference in IL-17,IL-12 level between the two groups (P 0.05). There was no correlation between serum Al level and cytokines IL-10 (r = 0.072, P = 0.4290.05) and IFN- 緯 (r = 0.480, P = 0.598). There was no significant difference in the expression of IgE between the AA group and the AA group in the high aluminum exposure area (P0.05). 6. The complement C4 level of the AA patients in the high aluminum exposed area was significantly lower than that in the non high aluminum exposed AA group (P0.01). There was no significant difference in complement C3 level between the two groups (P0.05). There was no correlation between serum aluminum and complement C4 (r = 0.097, P = 0.311). Conclusion: the level of serum aluminum in high aluminum exposed area is higher than that in non high aluminum exposed area. High aluminum exposure may affect immune function in patients with AA. The functional characteristics of AA immune cells in high aluminum exposed areas were significantly different from those in non high aluminum exposed areas. The changes of cellular immunity and immune factors were significant in AA, exposed to high aluminum. There was no correlation between serum aluminum levels and immune cells and immune factors.
【學(xué)位授予單位】：右江民族醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R556.5
[Abstract]:Aim: to study the changes of immune cell function and its clinical characteristics in patients with aplastic anemia (aplastic anemia,AA) exposed to high aluminum, and to analyze the relationship between serum aluminum levels and immune cells and immune factors. Methods: 62 cases of AA were selected and divided into two groups according to the aluminum content of drinking water and the source of the cases. The drinking water aluminum content was 300 渭 g / L and lived for a long time in the high aluminum exposed area of AA group (33 cases). Drinking water aluminum content 300ug/L and long-term living in non-aluminum industrial areas are non-high aluminum exposed areas of AA group, a total of 29 cases, and high aluminum exposure areas and non-high aluminum exposure areas 30 healthy people as the control group. The serum aluminum level was measured by inductively coupled plasma mass spectrometry (inductivity coupled plasma mass spectrometry,ICP-MS), and the expression of cytokines IL-10,IL-12,IL-17 and INF- 緯 was detected by enzyme-linked immunosorbent assay (enzyme linked immuno sorbent assay,ELISA). Flow cytometry (flow cytometry,FCM) was used to detect the proportion of lymphocyte subsets. The data of immunoglobulin, complement C3, C4 expression and clinical data were collected. To analyze the changes of immune cells and immune factors in AA patients exposed to high aluminum, and to explore the relationship between serum aluminum and immune indexes. Results: 1. The level of serum aluminum in high aluminum area (high aluminum AA and high aluminum healthy group) was higher than that in non high aluminum area population (non high aluminum AA and non high aluminum health group) (P0.05). The level of serum aluminum in AA patients in high aluminum exposure area was significantly higher than that in non high aluminum exposed area AA group and healthy control group (P0.01). 2. The anemia degree of AA patients in high aluminum exposed area was significantly higher than that in non high aluminum exposed area (P < 0 01). The degree of bone marrow hemopoietic tissue decrease was more severe than that in AA group (P0.05), but the age, sex, leukocyte, neutrophil, reticulocyte and platelet count in the two groups were significantly higher than those in the control group (P0.05). There was no significant difference in myeloproliferative degree. 3. The ratio of CD4 T lymphocytes and CD4 / CD8 in AA patients exposed to high aluminum were higher than those in AA group and healthy control group in non-aluminum exposed area (P 0.05). The percentage of CD8 T lymphocyte was lower than that of AA group and healthy control group (all P0.05), and there was no significant difference in CD3, CD 19, NK cell ratio between the two groups (P0.05). CD4 T, CD8 T lymphocyte ratio was not significantly different between the two groups (P 0.05). There was no correlation between CD4 / CD8 and serum aluminum level (r = 0.169, P = 0.067). 0. 072, 0. 438, 0. 072, 0. 438; (4) the level of IL-10,IFN- 緯 in AA patients exposed to high aluminum was higher than that in AA patients without high aluminum exposure (P 0.05). There was no significant difference in IL-17,IL-12 level between the two groups (P 0.05). There was no correlation between serum Al level and cytokines IL-10 (r = 0.072, P = 0.4290.05) and IFN- 緯 (r = 0.480, P = 0.598). There was no significant difference in the expression of IgE between the AA group and the AA group in the high aluminum exposure area (P0.05). 6. The complement C4 level of the AA patients in the high aluminum exposed area was significantly lower than that in the non high aluminum exposed AA group (P0.01). There was no significant difference in complement C3 level between the two groups (P0.05). There was no correlation between serum aluminum and complement C4 (r = 0.097, P = 0.311). Conclusion: the level of serum aluminum in high aluminum exposed area is higher than that in non high aluminum exposed area. High aluminum exposure may affect immune function in patients with AA. The functional characteristics of AA immune cells in high aluminum exposed areas were significantly different from those in non high aluminum exposed areas. The changes of cellular immunity and immune factors were significant in AA, exposed to high aluminum. There was no correlation between serum aluminum levels and immune cells and immune factors.
【學(xué)位授予單位】：右江民族醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R556.5
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