【摘要】：目的:通過(guò)研究缺氧誘導(dǎo)因子-1α(hypoxia-inducible factor-1 alpha,HIF-1α)及血管內(nèi)皮生長(zhǎng)因子(vascular endothelial growth factor,VEGF)在缺氧性肺動(dòng)脈高壓(hypoxia-induced plmonary hypertension,HPH)新生大鼠肺組織中的mRNA和蛋白質(zhì)表達(dá)以及與平均肺動(dòng)脈壓力(mean pulmonary artery pressure,m PAP)的關(guān)系,探討HIF-1α和VEGF在HPH發(fā)病機(jī)制中的作用。方法:把80只Wistar新生鼠根據(jù)隨機(jī)數(shù)字表法劃分成HPH組和空白組(C組),分別在造模3、7、14、21天時(shí)取HPH組和實(shí)驗(yàn)組各10只大鼠,通過(guò)檢測(cè)mPAP判斷造模是否成功;處死大鼠后測(cè)定HIF-1α及VEGF mRNA在肺組織的表達(dá)水平,利用Western blot方法測(cè)定HIF-1α及VEGF的蛋白表達(dá)進(jìn)行分析。結(jié)果:1.造模3、7、14、21天時(shí),HPH組mPAP較空白組顯著增高,差異有統(tǒng)計(jì)學(xué)意義(P0.05);2.造模3、7、14天時(shí)HPH組肺組織HIF-1α的mRNA表達(dá)較空白組升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05),造模7、14、21天時(shí),HPH組VEGF mRNA表達(dá)水平均明顯高于空白組(P0.05)。3.造模7天時(shí)HPH組肺組織HIF-1α蛋白表達(dá)顯著比空白組增多(P0.05);缺氧7、14、21天時(shí),HPH組VEGF蛋白表達(dá)均顯著比空白組增多(P0.05)。相關(guān)分析研究顯示,HPH組在造模第3、7、14、21天時(shí)HIF-1α的蛋白表達(dá)與mPAP變化大致成正相關(guān)(P0.05),造模第7、14、21天,VEGF蛋白質(zhì)表達(dá)與mPAP變化大致成正相關(guān)。結(jié)論VEGF作為HIF-lα的下游調(diào)控因子在新生大鼠HPH疾病發(fā)生發(fā)展過(guò)程里起到重要作用。
[Abstract]:Objective: to investigate the role of hypoxia inducible factor-1 偽 (hypoxia-inducible factor-1 alpha,HIF-1 偽) and vascular endothelial growth factor (vascular endothelial growth factor,VEGF) in hypoxic pulmonary hypertension (hypoxia-induced plmonary hypertension,). To explore the role of HIF-1 偽 and VEGF in the pathogenesis of HPH. Methods: 80 neonatal Wistar rats were randomly divided into HPH group and blank group (C group). 10 rats in HPH group and 10 rats in experimental group were taken from HPH group and experimental group on day 21 of model making. MPAP was detected to determine whether the model was successful or not. The expression of HIF-1 偽 and VEGF mRNA in lung tissue was determined after the rats were killed, and the protein expression of HIF-1 偽 and VEGF was analyzed by Western blot method. Results: 1. The mPAP of HPH group was significantly higher than that of blank group (P0.05). The mRNA expression of HIF-1 偽 in lung tissue of HPH group was significantly higher than that of blank group on day 14 (P0.05), and the expression level of VEGF mRNA in HPH group was significantly higher than that in control group (P0.05) at day 21 (P0.05). The expression of HIF-1 偽 protein in lung tissue of HPH group was significantly higher than that of blank group at 7 days (P0.05), and the expression of VEGF protein in HPH group was significantly higher than that in blank group at day 1421 of hypoxia (P0.05). Correlation analysis showed that the protein expression of HIF-1 偽 was positively correlated with the changes of mPAP in the HPH group on the 3rd day (P0.05), and the expression of VEGF protein was positively correlated with the change of mPAP on the 21st day (P0.05). Conclusion VEGF, as the downstream regulatory factor of HIF-l 偽, plays an important role in the pathogenesis and development of HPH in neonatal rats.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R544.1

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