【摘要】：目的:探究短肽GFS-4自組裝形成的水凝膠作為支架材料構(gòu)建三維微環(huán)境對(duì)BMSCs生物學(xué)特性及向心肌細(xì)胞方向誘導(dǎo)分化過程的影響。方法:剛果紅染色、紅細(xì)胞膜裂解實(shí)驗(yàn)檢測(cè)短肽GFS-4自組裝效果及對(duì)細(xì)胞膜是否具有裂解作用;CCK8和AO/EB染色分別檢測(cè)對(duì)BMSCs活性和凋亡的影響;Real-time PCR分析BMSCs誘導(dǎo)分化后MLC-2v、GATA-4基因表達(dá)情況。結(jié)果:GFS-4自組裝后形成致密凝膠,自組裝前后對(duì)細(xì)胞膜無損傷;三維培養(yǎng)環(huán)境細(xì)胞呈球形生長,細(xì)胞活力和凋亡速度均低于二維培養(yǎng)環(huán)境。三維培養(yǎng)組在誘導(dǎo)分化過程中的第5天和第7天MLC-2v、GATA-4基因表達(dá)均顯著高于二維組(P0.05)。結(jié)論:短肽GFS-4自組裝水凝膠構(gòu)建的三維微環(huán)境延緩了BMSCs的增殖速度和凋亡速度,并促進(jìn)向心肌方向誘導(dǎo)分化過程中MLC-2v、GATA-4基因的表達(dá)。
[Abstract]:Aim: to investigate the effects of hydrogels formed by self-assembly of short peptide GFS-4 as scaffold materials on the biological characteristics of BMSCs and its differentiation to cardiomyocytes. Methods: Congo red staining, erythrocyte membrane splitting assay were used to detect the effect of short peptide GFS-4 on self-assembly and cell membrane cleavage, and the effects of CCK8 and AO/EB staining on the activity and apoptosis of BMSCs, respectively. MLC-2v,GATA-4 gene expression after differentiation induced by BMSCs was analyzed by Real-time PCR. Results: after GFS-4 self-assembly, a dense gel was formed, and the cell membrane was not damaged before and after self-assembly, and the cells in three-dimensional culture environment were spherical, and the cell viability and apoptosis rate were lower than those in two-dimensional culture environment. The expression of MLC-2v,GATA-4 gene in three-dimensional culture group was significantly higher than that in two-dimensional group on the 5th and 7th day of differentiation (P0.05). Conclusion: Three-dimensional microenvironment constructed by self-assembled hydrogel with short peptide GFS-4 can delay the proliferation and apoptosis of BMSCs and promote the expression of MLC-2v,GATA-4 gene in the process of inducing differentiation into myocardium.
【作者單位】： 重慶醫(yī)科大學(xué)第一臨床學(xué)院;重慶醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院分子醫(yī)學(xué)與腫瘤研究中心;義烏市中心醫(yī)院溫州醫(yī)科大學(xué)附屬義烏醫(yī)院;鄭州市第七人民醫(yī)院;
【基金】：國家自然科學(xué)基金(31540019,31771101) 重慶市科委自然科學(xué)基金(cstc2015jcyj BX0072)資助項(xiàng)目
【分類號(hào)】：R542.22

【相似文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 王健;多模態(tài)成像檢測(cè)干細(xì)胞聯(lián)合功能化自組裝多肽治療急性心肌梗死[D];北京協(xié)和醫(yī)學(xué)院;2017年

相關(guān)碩士學(xué)位論文 前2條

1 袁霄;構(gòu)建自組裝肽納米纖維水凝膠用于心肌修復(fù)[D];重慶醫(yī)科大學(xué);2016年

2 陳振銀;新型自組裝短肽對(duì)心肌梗死組織修復(fù)功能的研究[D];重慶醫(yī)科大學(xué);2017年

，

本文編號(hào)：2393879