【摘要】：目的國內(nèi)外關(guān)于間隙性連接蛋白37(Cx37)基因改變巨噬細(xì)胞內(nèi)外的電流密度,使得動(dòng)脈粥樣硬化進(jìn)一步發(fā)展的研究甚少。文中旨在觀察Cx37對(duì)動(dòng)脈粥樣硬化中巨噬細(xì)胞電流密度的影響。方法選取30只Wistar大鼠,Cx37+(保留Cx37基因)、Cx37-(沉默Cx37基因)各15只。采用高脂飲食建立動(dòng)脈粥樣硬化模型;根據(jù)標(biāo)本采集部位分為4組:Cx37+斑塊組、Cx37-斑塊組(取自斑塊)及Cx37+血液組、Cx37-血液組(取自血液),用RT-PCR檢測(cè)Cx37+大鼠不同部位Cx37的表達(dá);分離Cx37+、Cx37-大鼠血液中及斑塊處單核巨噬細(xì)胞并培養(yǎng),用全細(xì)胞記錄法測(cè)定電流密度并進(jìn)行比較。結(jié)果 Cx37+大鼠斑塊中單核巨噬細(xì)胞中Cx37的相對(duì)表達(dá)水平顯著高于血液[(1.1±0.02)vs(0.6±0.03)],差異有統(tǒng)計(jì)學(xué)意義(P=0.010)。Cx37+、Cx37-大鼠均行能譜CT檢測(cè)鼠頸動(dòng)脈斑塊檢測(cè),驗(yàn)證了動(dòng)物模型的成功建立。80、120、160 ms時(shí),Cx37+斑塊組電流密度[(0.61±0.06)、(0.67±0.07)、(0.91±0.03)A/cm~2]顯著高于Cx37+血液組[(0.49±0.02)、(0.61±0.03)、(0.67±0.02)A/cm~2]、Cx37-斑塊組[(0.48±0.02)、(0.60±0.02)、(0.64±0.02)A/cm~2]、Cx37-血液組[(0.49±0.02)、(0.59±0.02)、(0.64±0.02)A/cm~2];200、240、320 ms時(shí)亦顯著高于Cx37+血液組、Cx37-斑塊組、Cx37-血液組(P0.05)。結(jié)論 Cx37對(duì)動(dòng)脈粥樣硬化中單核巨噬細(xì)胞斑塊處電流密度的影響比外周血液更為顯著,可促使巨噬細(xì)胞繼續(xù)活化,動(dòng)脈粥樣硬化進(jìn)一步發(fā)展。
[Abstract]:Objective to investigate the effect of gap junction protein 37 (Cx37) gene on the current density of macrophages and make the further development of atherosclerosis rare. The aim of this study was to observe the effect of Cx37 on the current density of macrophages in atherosclerosis. Methods 30 Wistar rats with Cx37 (Cx37 gene) and Cx37- (silent Cx37 gene) were selected. Atherosclerosis model was established by high-fat diet. The samples were divided into four groups: Cx37 plaque group, Cx37- plaque group (taken from plaque) and Cx37 blood group, Cx37- blood group (taken from blood). The expression of Cx37 in different parts of Cx37 rats was detected by RT-PCR. Mononuclear macrophages were isolated from the blood and plaque of Cx37 and Cx37- rats and cultured. The current density was measured by whole-cell recording method and compared. Results the relative expression of Cx37 in mononuclear macrophages in plaque of Cx37 rats was significantly higher than that in blood [(1.1 鹵0.02) vs (0.6 鹵0.03)], and the difference was statistically significant (P0. 010). Cx37. Cx37- rats were detected carotid plaque by energy dispersive CT. The results showed that the current density of Cx37 plaque group was (0.61 鹵0.06), (0.67 鹵0.07) at 80120160 ms. (0. 91 鹵0. 03) A/cm~2 was significantly higher than that in Cx37 blood group [(0. 49 鹵0. 02), () 0. 61 鹵0. 03), (0. 67 鹵0. 02 A/cm~2], Cx37- plaque group [(0. 48 鹵0. 02), () 0. 60 鹵0. 02]. (0. 64 鹵0. 02 A/cm~2), Cx37- blood group [(0. 49 鹵0. 02), () 0. 59 鹵0. 02), (0. 64 鹵0. 02 A/cm~2]; At 200240320 ms, it was also significantly higher than that in Cx37 blood group, Cx37- plaque group and Cx37- blood group (P0.05). Conclusion the effect of Cx37 on the current density in plaque of mononuclear macrophages in atherosclerosis is more significant than that in peripheral blood, which can promote the activation of macrophages and the further development of atherosclerosis.
【作者單位】： 云南大學(xué)博士后流動(dòng)站;無錫市人民醫(yī)院心臟中心;昆明醫(yī)科大學(xué)第一附屬醫(yī)院心內(nèi)科;
【分類號(hào)】：R543.5

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