【摘要】：目的:探討成人EB病毒相關(guān)淋巴細胞克隆性轉(zhuǎn)化的實驗方法,為EB病毒相關(guān)淋巴細胞增殖病的早期診斷、分類、預(yù)測轉(zhuǎn)歸提供客觀的判斷指標。方法:采集5例成人EB病毒相關(guān)的淋巴組織增殖性疾病(EBV+LPD)患者的外周血標本,另采集4例成人傳染性單核細胞增多癥(IM)外周血標本作為陰性對照,3例急性NK細胞白血病(ANKL)的外周血標本作為陽性對照。應(yīng)用流式細胞術(shù)(FCM)檢測淋巴細胞免疫表型、RT-PCR檢測T細胞受體(TCR)基因重排,Southern blot方法鑒定EBV末端重復(fù)序列(TR)多態(tài)性,對患者外周淋巴細胞進行了克隆性分析。結(jié)果:在5例EBV-LPD患者中,FCM僅檢測出1例TCRVβ表型呈克隆性;RT-PCR檢測TCRVβ時在IM患者均檢測出TCR受體基因重排陽性,在5例EBV-LPD患者中檢測出4例陽性;在5例EBV+LPD患者中1例檢測有單克隆條帶,2例檢測有寡克隆條帶。結(jié)論:流式細胞術(shù)表型分析的敏感性較差;TCRVβ重排不能區(qū)分反應(yīng)性克隆和轉(zhuǎn)化型克隆;而EB病毒末端重復(fù)序列克隆性鑒定的方法,能夠更客觀特異地反應(yīng)EB病毒相關(guān)淋巴細胞增殖的克隆性轉(zhuǎn)化,有助于改善疾病的早期診斷、分類及預(yù)測臨床轉(zhuǎn)歸。
[Abstract]:Objective: to investigate the experimental method of clonal transformation of adult EB virus-associated lymphocytes in order to provide an objective index for the early diagnosis, classification and prognosis of EB virus-associated lymphoproliferative disease. Methods: peripheral blood samples of 5 adult patients with EB virus-associated lymphoproliferative disease (EBV LPD) and 4 adult infectious mononucleosis (IM) peripheral blood samples were collected as negative control. Peripheral blood samples from 3 patients with acute NK cell leukemia (ANKL) were used as positive control. The lymphocyte immunophenotype was detected by flow cytometry (FCM), and the (TR) polymorphism at the end of EBV was identified by RT-PCR analysis of T cell receptor (TCR) gene rearrangement, Southern blot. The clonal analysis of peripheral lymphocytes was carried out. Results: in 5 cases of EBV-LPD, only 1 case of TCRV 尾 was detected in FCM, TCRV 尾 was detected in all cases of IM by RT-PCR, and 4 cases were positive in 5 cases of EBV-LPD. Monoclonal bands and oligoclonal bands were detected in 1 of 5 patients with EBV LPD. Conclusion: the sensitivity of phenotypic analysis by flow cytometry is poor, and TCRV 尾 rearrangement can not distinguish reactive clone from transformed clone. The clone identification method of terminal repeat sequence of EB virus can more objectively and specifically reflect the clonal transformation of lymphocyte proliferation associated with EB virus, which is helpful to improve the early diagnosis, classification and prediction of clinical outcome of EB virus.
【作者單位】： 北京大學(xué)深圳醫(yī)院血液內(nèi)科;
【分類號】：R55

【相似文獻】

相關(guān)期刊論文 前10條

1 李守新,徐慧珍;急性淋巴細胞白血病細胞克隆性分析的臨床意義[J];內(nèi)科急危重癥雜志;1997年04期

2 李挺;祖幼立;;抗原受體基因重排克隆性分析淋巴瘤輔助診斷技術(shù)的發(fā)展和應(yīng)用現(xiàn)狀[J];北京大學(xué)學(xué)報(醫(yī)學(xué)版);2008年04期

3 李揚秋,許敏華,陳少華,郁志,曾慧蘭,盧育洪,楊力建,朱平;自身免疫性溶血性貧血病人T細胞的寡克隆性[J];中國免疫學(xué)雜志;2000年09期

4 李克;惡性血液病克隆性研究近況[J];國外醫(yī)學(xué).輸血及血液學(xué)分冊;1996年04期

5 朱平;;移植后淋巴細胞增殖病的病因和臨床診治[J];中華醫(yī)學(xué)雜志;2006年42期

6 謝正德;;EB病毒相關(guān)噬血淋巴組織細胞增生癥[J];中國實用兒科雜志;2010年08期

7 趙曉紅,姜偉,紀祥瑞;EB病毒在組織細胞壞死性淋巴結(jié)炎發(fā)病中的作用[J];青島大學(xué)醫(yī)學(xué)院學(xué)報;2004年01期

8 長晟;;與EB病毒有關(guān)的中樞神經(jīng)系統(tǒng)淋巴瘤[J];國外醫(yī)學(xué)情報;1984年07期

9 杜濤,許彩民,朱蘇玲,徐文瑾,潘華珍,張之南;經(jīng)EB病毒轉(zhuǎn)染建立陣發(fā)性睡眠性血紅蛋白尿癥永生細胞系的方法改進[J];中國實驗血液學(xué)雜志;1999年04期

10 黃梅娟,李揚秋,陳少華,楊力建,周羽z，

本文編號：2357826