【摘要】：目的初步探討組蛋白去乙�；�(HDAC8)在鹽酸異丙腎上腺素(ISO)誘導(dǎo)的大鼠心肌纖維化中表達(dá)的機(jī)制。方法將40只雄性SD大鼠隨機(jī)均等分為正常組和模型組。模型組給予ISO 1次/d(5 mg/kg),正常組予以等劑量生理鹽水皮下注射,連續(xù)注射7 d后處死大鼠取血液標(biāo)本,并取心肌組織。測定心臟質(zhì)量指數(shù);ELISA法檢測血清中I型膠原和Ⅲ型膠原的含量;HE和Masson染色法觀察心肌纖維化程度;Western blot法測定HDAC8和α-平滑肌肌動蛋白(α-SMA)蛋白表達(dá)情況;qRT-PCR法檢測HDAC8 mRNA的表達(dá)。結(jié)果與正常組比較,模型組心臟質(zhì)量指數(shù)明顯增加(P0.05);HE和Masson染色顯示模型組心肌組織出現(xiàn)明顯的膠原纖維增生;模型組血清標(biāo)本中Ⅰ型膠原和Ⅲ型膠原的含量較正常組明顯增加(P0.05);Western blot法檢測顯示模型組HDAC8、α-SMA的蛋白表達(dá)明顯高于正常組(P0.05,P0.01);qRT-PCR檢測模型組HDAC8 mRNA表達(dá)明顯高于正常組(P0.05)。結(jié)論 HDAC8在心肌纖維化中高表達(dá),而在正常心肌組織中低表達(dá),提示其在心肌纖維化的發(fā)生發(fā)展中可能發(fā)揮作用。
[Abstract]:Objective to investigate the mechanism of histone deacetylase (HDAC8) expression in myocardial fibrosis induced by isoproterenol (ISO) in rats. Methods 40 male SD rats were randomly divided into normal group and model group. The model group was given ISO once a day (5 mg/kg), and the normal group was subcutaneously injected with the same dose of normal saline. After 7 days of continuous injection, the rats were sacrificed to get blood samples and myocardial tissue. Cardiac mass index, serum type I collagen and type 鈪，

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