【摘要】：目的急性心肌梗死是世界發(fā)病率及致死率最高的疾病之一。而心肌梗死后心肌纖維化是急性心肌梗死患者心功能不全的重要機制。微小RNA是一種非編碼RNA,可調(diào)控基因表達,與急性心肌梗死密切相關(guān)。本文旨在探討microRNA-150在心肌梗死大鼠的心肌組織中的表達,并進一步研究miR-150對心肌梗死后心肌纖維化及心臟功能的作用。方法通過結(jié)扎Sprague Dawley大鼠心臟冠狀動脈左前降支構(gòu)造心肌梗死模型,采用過表達microRNA-150的慢病毒上調(diào)大鼠心肌組織中的miR-150表達水平,進一步通過RT-PCR檢測心肌梗死邊緣區(qū)microRNA-150的表達及col1ɑ1和α-SMA的信使RNA的含量,通過Western Blotting檢測心肌梗死邊緣區(qū)col1ɑ1、α-SMA蛋白的水平,通過心臟彩超檢查及Masson染色分別檢測心臟功能及心肌纖維化,研究microRNA-150在心肌梗死大鼠心肌組織中的表達及其對心肌梗死后纖維化及心臟功能的影響。結(jié)果實驗結(jié)果表明microRNA-150在心肌梗死術(shù)后第28天梗死邊緣區(qū)心肌中表達降低(P0.05)。此外,上調(diào)心肌梗死大鼠心肌組織中miRNA-150的表達水平可改善心肌梗死后心功能,降低膠原容積分數(shù)并且降低心肌組織中col1ɑ1及ɑ-SMA的表達(P0.001)結(jié)論MicroRNA-150在心肌梗死邊緣區(qū)心肌組織中表達是下降的,而上調(diào)心肌組織中microRNA-150的表達可抑制心肌梗死后纖維化并改善心臟功能。
[Abstract]:Objective Acute myocardial infarction (AMI) is one of the diseases with the highest morbidity and mortality in the world. Myocardial fibrosis after myocardial infarction is an important mechanism of cardiac insufficiency in patients with acute myocardial infarction. Micro RNA is a non-coding RNA, regulated gene expression, which is closely related to acute myocardial infarction. The purpose of this study was to investigate the expression of microRNA-150 in myocardial tissue of rats with myocardial infarction and to further study the effects of miR-150 on myocardial fibrosis and cardiac function after myocardial infarction. Methods the myocardial infarction model was constructed by ligating the left anterior descending branch of coronary artery in Sprague Dawley rats. The lentivirus overexpression of microRNA-150 was used to up-regulate the expression of miR-150 in myocardial tissue of rats. Furthermore, the expression of microRNA-150 and the content of col1 1 and 偽-SMA messenger RNA were detected by RT-PCR, and the levels of col1 1, 偽-SMA protein were detected by Western Blotting. Cardiac function and myocardial fibrosis were detected by color Doppler echocardiography and Masson staining. The expression of microRNA-150 in myocardial tissue of rats with myocardial infarction and its effect on myocardial fibrosis and cardiac function after myocardial infarction were studied. Results the results showed that the expression of microRNA-150 in the myocardium of myocardial infarction margin was decreased on the 28th day after myocardial infarction (P0.05). In addition, upregulating the expression of miRNA-150 in myocardial tissue of myocardial infarction rats can improve the cardiac function after myocardial infarction. Decrease of collagen volume fraction and expression of col1 1 and SMA in myocardial tissue (P0. 001) conclusion the expression of MicroRNA-150 in myocardial tissue of myocardial infarction marginal area is decreased. Upregulating the expression of microRNA-150 in myocardial tissue can inhibit fibrosis and improve cardiac function after myocardial infarction.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R542.22

【參考文獻】

相關(guān)期刊論文 前10條

1 呂洪雪;王婷;葉小漢;吳錦波;蘇志遠;;心康片對阿霉素誘導的心衰大鼠心肌細胞凋亡率、膠原容積分數(shù)、肌漿網(wǎng)Ca~(2+)-ATP酶活性的影響[J];廣州中醫(yī)藥大學學報;2017年02期

2 李巍偉;孔鵬;韓梅;;lncRNAs在心血管疾病發(fā)生中的作用[J];中國生物化學與分子生物學報;2016年10期

3 陳偉偉;高潤霖;劉力生;朱曼璐;王文;王擁軍;吳兆蘇;李惠君;顧東風;楊躍進;鄭哲;蔣立新;胡盛壽;;《中國心血管病報告2015》概要[J];中國循環(huán)雜志;2016年06期

4 ;急性冠脈綜合征急診快速診療指南[J];中華急診醫(yī)學雜志;2016年04期

5 彭元亮;孫志衛(wèi);韓旭;楊秦;劉靜;;miR-150在造血發(fā)育中的功能研究[J];生命科學;2015年09期

6 李磊;夏豪;王欣;童隨陽;;miRNA150與心血管疾病的研究進展[J];醫(yī)學研究雜志;2015年06期

7 耿傳良;別自東;;微小RNA與心肌梗死后心臟重塑研究進展[J];山西醫(yī)藥雜志;2015年03期

8 劉付梅;李祥勇;周克元;;miR-150與臨床疾病研究的新進展[J];醫(yī)學研究生學報;2014年02期

9 ZHAO Na;YU HaiYi;YU HaiTao;SUN Min;ZHANG YouYi;XU Ming;GAO Wei;;miRNA-711-SP1-collagen-I pathway is involved in the anti-fibrotic effect of pioglitazone in myocardial infarction[J];Science China(Life Sciences);2013年05期

10 王亞靜;吳新華;;心肌梗死后心力衰竭發(fā)生機制的研究進展[J];疑難病雜志;2012年09期

相關(guān)博士學位論文 前1條

1 張瑞;血液循環(huán)中與急性心肌梗死相關(guān)聯(lián)miRNA的探索[D];鄭州大學;2016年

，

本文編號：2300324