【摘要】：目的急性心肌梗死是世界發(fā)病率及致死率最高的疾病之一。而心肌梗死后心肌纖維化是急性心肌梗死患者心功能不全的重要機(jī)制。微小RNA是一種非編碼RNA,可調(diào)控基因表達(dá),與急性心肌梗死密切相關(guān)。本文旨在探討microRNA-150在心肌梗死大鼠的心肌組織中的表達(dá),并進(jìn)一步研究miR-150對心肌梗死后心肌纖維化及心臟功能的作用。方法通過結(jié)扎Sprague Dawley大鼠心臟冠狀動(dòng)脈左前降支構(gòu)造心肌梗死模型,采用過表達(dá)microRNA-150的慢病毒上調(diào)大鼠心肌組織中的miR-150表達(dá)水平,進(jìn)一步通過RT-PCR檢測心肌梗死邊緣區(qū)microRNA-150的表達(dá)及col1ɑ1和α-SMA的信使RNA的含量,通過Western Blotting檢測心肌梗死邊緣區(qū)col1ɑ1、α-SMA蛋白的水平,通過心臟彩超檢查及Masson染色分別檢測心臟功能及心肌纖維化,研究microRNA-150在心肌梗死大鼠心肌組織中的表達(dá)及其對心肌梗死后纖維化及心臟功能的影響。結(jié)果實(shí)驗(yàn)結(jié)果表明microRNA-150在心肌梗死術(shù)后第28天梗死邊緣區(qū)心肌中表達(dá)降低(P0.05)。此外,上調(diào)心肌梗死大鼠心肌組織中miRNA-150的表達(dá)水平可改善心肌梗死后心功能,降低膠原容積分?jǐn)?shù)并且降低心肌組織中col1ɑ1及ɑ-SMA的表達(dá)(P0.001)結(jié)論MicroRNA-150在心肌梗死邊緣區(qū)心肌組織中表達(dá)是下降的,而上調(diào)心肌組織中microRNA-150的表達(dá)可抑制心肌梗死后纖維化并改善心臟功能。
[Abstract]:Objective Acute myocardial infarction (AMI) is one of the diseases with the highest morbidity and mortality in the world. Myocardial fibrosis after myocardial infarction is an important mechanism of cardiac insufficiency in patients with acute myocardial infarction. Micro RNA is a non-coding RNA, regulated gene expression, which is closely related to acute myocardial infarction. The purpose of this study was to investigate the expression of microRNA-150 in myocardial tissue of rats with myocardial infarction and to further study the effects of miR-150 on myocardial fibrosis and cardiac function after myocardial infarction. Methods the myocardial infarction model was constructed by ligating the left anterior descending branch of coronary artery in Sprague Dawley rats. The lentivirus overexpression of microRNA-150 was used to up-regulate the expression of miR-150 in myocardial tissue of rats. Furthermore, the expression of microRNA-150 and the content of col1 1 and 偽-SMA messenger RNA were detected by RT-PCR, and the levels of col1 1, 偽-SMA protein were detected by Western Blotting. Cardiac function and myocardial fibrosis were detected by color Doppler echocardiography and Masson staining. The expression of microRNA-150 in myocardial tissue of rats with myocardial infarction and its effect on myocardial fibrosis and cardiac function after myocardial infarction were studied. Results the results showed that the expression of microRNA-150 in the myocardium of myocardial infarction margin was decreased on the 28th day after myocardial infarction (P0.05). In addition, upregulating the expression of miRNA-150 in myocardial tissue of myocardial infarction rats can improve the cardiac function after myocardial infarction. Decrease of collagen volume fraction and expression of col1 1 and SMA in myocardial tissue (P0. 001) conclusion the expression of MicroRNA-150 in myocardial tissue of myocardial infarction marginal area is decreased. Upregulating the expression of microRNA-150 in myocardial tissue can inhibit fibrosis and improve cardiac function after myocardial infarction.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R542.22

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