【摘要】：目的闡明腫瘤壞死因子α轉(zhuǎn)化酶(ADAM17)調(diào)控降鈣素基因相關(guān)肽(CGRP)的抗血管平滑肌細胞(VSMC)增殖效應。方法以大鼠源性胸主動脈平滑肌細胞株(A10VSMC)為研究對象,采用RNA干擾抑制ADAM17表達;采用蛋白質(zhì)免疫印跡和實時定量PCR檢測蛋白和mRNA表達水平;采用噻唑藍比色法評估細胞活性。結(jié)果 CGRP顯著抑制血管緊張素Ⅱ介導的VSMC增殖。血管緊張素Ⅱ刺激24 h后,VSMC中ADAM17表達顯著增加。CGRP預處理30 min能顯著降低VSMC中ADAM17表達,而CGRP受體拮抗劑CGRP8-37可抑制或逆轉(zhuǎn)上述效應。ADAM17 RNA干擾可顯著抑制血管緊張素Ⅱ介導的VSMC增殖。結(jié)論降低ADAM17表達可能是CGRP抑制血管緊張素Ⅱ介導的VSMC增殖效應的機制。
[Abstract]:Aim to elucidate the effect of tumor necrosis factor alpha converting enzyme (ADAM17) on the proliferation of vascular smooth muscle cells (VSMCs) regulated by calcitonin gene-related peptide (CGRP). Methods Rat thoracic aortic smooth muscle cell line (A10VSMC) was used to inhibit ADAM17 expression by RNA interference, protein and mRNA expression levels were detected by Western blot and real-time quantitative PCR, and cell activity was evaluated by thiazolyl blue colorimetry. Results CGRP significantly inhibited angiotensin 鈪，

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