【摘要】：目的定期輸血患者血清抗體測定和特異性分析。方法采用微柱凝膠抗人球卡方法,對(duì)354例長期定期輸血的β-地中海貧血患者,進(jìn)行血清抗體篩查和抗體鑒定。結(jié)果 354人中,28人抗體陽性(7.9%),其中13人檢出同種抗體(1人無法確定抗體特異性),15人檢出自身抗體。共檢出特異性同種抗體19個(gè),其中Rh血型抗體15個(gè)(78.9%),包括抗-c、抗-E、抗-C和抗-Cw;自身抗體中,1例具有特異性,為抗-C,5例血清與所有鑒定細(xì)胞強(qiáng)凝集,均報(bào)輸血不良反應(yīng),其余9人血清與同種細(xì)胞弱反應(yīng),均未報(bào)輸血反應(yīng)。另外,觀察到18人次側(cè)配血凝集(5.1%),但血清不與同種細(xì)胞反應(yīng)即主側(cè)配血相合,或因多次輸血異體血漿蛋白吸附或藥物抗體等原因引起,均未報(bào)輸血反應(yīng)。結(jié)論長期定期輸血的地中海貧血患者血清同種抗體和自身抗體的比率較高,同種抗體以Rh血型抗體為主,應(yīng)進(jìn)行Rh Cc Ee相合輸血。
[Abstract]:Objective to determine the serum antibody and its specificity in patients with regular blood transfusion. Methods Serum antibody screening and antibody identification were performed in 354 patients with 尾-thalassemia who received blood transfusion for a long time by microcolumn gel anti-human ball card method. Results of 354 cases, 28 were positive (7.9%), of which 13 were homoantibodies (1 could not determine the specificity of antibodies) and 15 were autoantibodies. A total of 19 specific homoantibodies were detected, of which 15 (78.9%) were Rh blood group antibodies, including anti-c, anti-E, anti-C and anti-Cw; autoantibodies. The blood transfusion reaction was not reported in the other 9 cases. In addition, 18 cases of hemagglutination (5.1%) were observed, but the blood did not match with the homologous cells, or was caused by multiple blood transfusions, such as plasma protein adsorption or drug antibody. No blood transfusion reaction was reported. Conclusion the ratio of serum autoantibodies and alloantibodies in thalassemia patients with chronic and regular blood transfusion is higher. Rh blood group antibody is the main antibody and Rh Cc Ee matching transfusion should be carried out.
【作者單位】： 深圳大學(xué)第一附屬醫(yī)院深圳市第二人民醫(yī)院;無錫市第五人民醫(yī)院;遼寧省血液中心;深圳市血液中心;
【基金】：深圳市科技創(chuàng)新項(xiàng)目(JCYJ20140414170821221) 深圳市第二人民醫(yī)院院級(jí)重點(diǎn)實(shí)驗(yàn)室項(xiàng)目資助
【分類號(hào)】：R556.61
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本文編號(hào)：2263895