【摘要】：目的探討辛伐他汀對(duì)動(dòng)脈粥樣硬化(AS)大鼠模型血管壁細(xì)胞凋亡、Bcl-2蛋白的表達(dá)干預(yù)作用。方法高脂飼料及腹腔注射維生素D_3復(fù)制大鼠AS模型,隨機(jī)分為對(duì)照組(n=10)給予普通飼料喂養(yǎng),實(shí)驗(yàn)組(n=13)給予高脂飼料喂養(yǎng),干預(yù)組(n=13)給予高脂飼料喂養(yǎng)基礎(chǔ)上加用辛伐他汀干預(yù),11周后取胸主動(dòng)脈,觀察其斑塊變化。采用免疫組化Elivision法測(cè)定AS血管壁中Bcl-2蛋白表達(dá)。通過(guò)末端脫氧核苷酸轉(zhuǎn)移酶介導(dǎo)的dUTP缺口末端標(biāo)記(TUNEL)法檢測(cè)細(xì)胞凋亡指數(shù)(AI),分析各組中Bcl-2表達(dá)及AI變化。結(jié)果與對(duì)照組比較,Bcl-2蛋白的表達(dá)在實(shí)驗(yàn)組明顯降低(P0.05);而與實(shí)驗(yàn)組比較,干預(yù)組Bcl-2蛋白的表達(dá)明顯升高(P0.05),且與對(duì)照組比較無(wú)差異。同時(shí),AI在各組中的變化比較則相反,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論辛伐他汀抗大鼠AS的作用可能與上調(diào)Bcl-2蛋白表達(dá),進(jìn)而抑制細(xì)胞凋亡相關(guān)。
[Abstract]:Objective to investigate the effect of simvastatin on the expression of Bcl-2 protein in vascular parietal cells of atherosclerotic (AS) rats. Methods the AS model of rats was induced by high fat diet and intraperitoneal injection of vitamin D 3. The rats were randomly divided into two groups: control group (n = 10) and experimental group (n = 13) were fed with high fat diet. The thoracic aorta was taken after 11 weeks of intervention with simvastatin on the basis of high fat diet in the intervention group (nong13), and the plaque changes were observed. The expression of Bcl-2 protein in vascular wall of AS was determined by immunohistochemical Elivision method. Terminal deoxynucleotidyl transferase-mediated dUTP Nick end labeling (TUNEL) was used to detect the expression of Bcl-2 and the change of AI in all groups by (AI),. Results compared with the control group, the expression of Bcl-2 protein was significantly decreased in the experimental group (P0.05), but the expression of Bcl-2 protein in the intervention group was significantly higher than that in the control group (P0.05), and there was no difference between the intervention group and the control group. At the same time, the changes of AI in each group were opposite, the differences were statistically significant (P0.05). Conclusion the effect of simvastatin on rat AS may be related to up-regulation of Bcl-2 protein expression and inhibition of apoptosis.
【作者單位】： 蚌埠醫(yī)學(xué)院第一附屬醫(yī)院心血管科;
【基金】：安徽高校研究重點(diǎn)項(xiàng)目(SK2016A0595) 蚌埠市科技局課題(20150336) 蚌埠醫(yī)學(xué)院科研課題資助(BY0855)
【分類號(hào)】：R543.5

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