天堂国产午夜亚洲专区-少妇人妻综合久久蜜臀-国产成人户外露出视频在线-国产91传媒一区二区三区

Syndecan-1對大鼠心肌成纖維細胞生物學行為影響

發(fā)布時間:2018-08-17 15:47
【摘要】:目的評估syndecan-1(Sdc1)對大鼠心肌成纖維細胞生物學行為的影響,為心肌梗死(MI)后心室重構(gòu)的防治提供依據(jù)。方法體外分離培養(yǎng)SD大鼠乳鼠心肌成纖維細胞,分別轉(zhuǎn)染Sdc1 siRNA質(zhì)粒和Sdc1高表達質(zhì)粒,并設(shè)立空白對照組、無關(guān)序列和空載質(zhì)粒作為陰性對照。采用流式細胞術(shù)檢測細胞增殖,CCK-8法檢測細胞活力,酶聯(lián)免疫法(ELISA)檢測膠原(羥脯氨酸)分泌能力及Transwell細胞遷移實驗檢測細胞遷移能力。結(jié)果Sdc1 siRNA組心肌成纖維細胞S期比例、增殖指數(shù)、細胞活力、膠原(羥脯氨酸)分泌量和遷移細胞數(shù)分別為(8.36±0.23)%、(13.2±0.5)%、(1.72±0.12)、(1162.3±60.4)pg/mL和170個/孔,明顯高于空白對照和陰性對照組(P0.01);Sdc1高表達質(zhì)粒組心肌成纖維細胞S期比例、增殖指數(shù)、細胞活力、膠原(羥脯氨酸)分泌量和遷移細胞數(shù)分別為(6.48±0.22)%、(10.8±0.6)%、(1.30±0.11)、(346.8±52.1)pg/mL和50個/孔,明顯低于空白對照和空載質(zhì)粒組(P0.01)。結(jié)論 Sdc1能夠抑制心肌成纖維細胞的增殖、活力、遷移和分泌能力。
[Abstract]:Objective to evaluate the effects of syndecan-1 (Sdc1) on the biological behavior of cardiac fibroblasts in rats and to provide evidence for the prevention and treatment of ventricular remodeling after myocardial infarction (MI). Methods SD rat myocardial fibroblasts were isolated and cultured in vitro and transfected with Sdc1 siRNA plasmid and Sdc1 high expression plasmid respectively. The blank control group was set up and the unrelated sequence and empty plasmid were used as negative control. The cell viability was detected by flow cytometry (FCM) and CCK-8 assay. The activity of collagen (hydroxyproline) was detected by enzyme-linked immunosorbent assay (ELISA) and the migration ability of Transwell cells was detected by Transwell cell migration assay. Results in Sdc1 siRNA group, the ratio of S phase, proliferation index, cell viability, secretion of collagen (hydroxyproline) and the number of migrating cells were (8.36 鹵0.23), (13.2 鹵0.5), (1.72 鹵0.12), () 1162.3 鹵60.4 pg/mL and 170 / well, respectively. The ratio of S phase, proliferation index, cell viability, collagen (hydroxyproline) secretion and the number of migrating cells were (6.48 鹵0.22), (10.8 鹵0.60), (1.30 鹵0.11), (鹵52.1) pg/mL and (50 / well), respectively. It was significantly lower than the blank control group and the blank plasmid group (P0.01). Conclusion Sdc1 can inhibit the proliferation, activity, migration and secretion of myocardial fibroblasts.
【作者單位】: 中山大學孫逸仙紀念醫(yī)院;
【基金】:廣東省科技計劃項目(2013B021800096)
【分類號】:R542.22
,

本文編號:2188128

資料下載
論文發(fā)表

本文鏈接:http://sikaile.net/yixuelunwen/xxg/2188128.html


Copyright(c)文論論文網(wǎng)All Rights Reserved | 網(wǎng)站地圖 |

版權(quán)申明:資料由用戶34ad6***提供,本站僅收錄摘要或目錄,作者需要刪除請E-mail郵箱bigeng88@qq.com
欧美一区二区三区不卡高清视| 高清亚洲精品中文字幕乱码| 国产精品日韩精品一区| 欧美丝袜诱惑一区二区| 在线精品首页中文字幕亚洲| 激情中文字幕在线观看 | 有坂深雪中文字幕亚洲中文| 欧美国产日韩在线综合| 欧美又大又黄刺激视频| 日本淫片一区二区三区| 亚洲中文字幕高清乱码毛片 | 色哟哟在线免费一区二区三区| 久久亚洲午夜精品毛片| 日韩精品小视频在线观看| 亚洲综合伊人五月天中文| 色综合久久中文综合网| 日本av在线不卡一区| 制服丝袜美腿美女一区二区| 亚洲中文字幕一区三区| 99久热只有精品视频免费看| 一区二区福利在线视频| 亚洲国产一级片在线观看| 成人免费视频免费观看| 一区二区三区18禁看| 欧美亚洲三级视频在线观看| 高清欧美大片免费在线观看| 91国内视频一区二区三区| 亚洲品质一区二区三区| 高清一区二区三区不卡免费| 极品熟女一区二区三区| 韩日黄片在线免费观看| 高清一区二区三区四区五区| 五月婷婷欧美中文字幕| 欧美小黄片在线一级观看| 亚洲中文字幕在线乱码av| 久久热在线视频免费观看| 粉嫩内射av一区二区| 91人妻久久精品一区二区三区| 嫩呦国产一区二区三区av| 办公室丝袜高跟秘书国产| 中日韩美一级特黄大片|