發(fā)布時間：2018-06-10 16:35

  本文選題：孕期炎癥 + 心肌纖維化　； 參考：《第三軍醫(yī)大學(xué)學(xué)報》2017年08期

【摘要】：目的探討孕期炎癥刺激對子代大鼠心肌纖維化的影響。方法 8只SD孕鼠采用隨機數(shù)字表法完全隨機分為:對照組(Con)和脂多糖(lipopolysaccharides,LPS)刺激組,每組4只。分別在孕第8、10、12天腹腔注射0.5 m L生理鹽水或LPS(0.79 mg/kg)。仔鼠6、8、10、12、16周齡測尾動脈血壓;組織切片Masson染色觀察心肌組織纖維化;透射電鏡觀察心肌顯微結(jié)構(gòu);實時熒光定量PCR檢測心肌組織縫隙連接蛋白(Connexin,Cx)43、微管相關(guān)蛋白1輕鏈3B(microtubule-associated protein 1light chain 3B,LC3B)、DNA甲基轉(zhuǎn)移酶-1(DNA methyltransferase 1,DNMT-1)mRNA表達水平;Western blot檢測Cx43、LC3B、DNMT-1蛋白表達。結(jié)果與Con組子代相比,LPS組子代大鼠6、8、10、12、16周齡時血壓均明顯升高(P0.01);心肌組織膠原沉積增多,細胞間縫隙連接減少,自噬小體數(shù)量增加;Cx43和DNMT-1 mRNA表達水平顯著增加(P0.05),而LC3B的mRNA表達水平顯著下降(P0.05);Cx43蛋白表達水平較Con組子代明顯降低(P0.05),LC3BⅡ/LC3BⅠ和DNMT-1的蛋白表達水平均明顯增加(P0.05)。結(jié)論孕期炎癥刺激可導(dǎo)致子代大鼠心肌纖維化。這可能與孕期炎癥刺激子代大鼠心肌組織自噬活化及Cx43蛋白表達降低相關(guān)。
[Abstract]:Objective to investigate the effect of inflammatory stimulation during pregnancy on myocardial fibrosis in offspring rats. Methods eight pregnant Sprague-Dawley rats were randomly divided into control group and lipopolysaccharide LPS-stimulated group with 4 rats in each group. 0.5 mL normal saline or 0.79 mg / kg LPSN were injected intraperitoneally on the 12th day of pregnancy on the 8th day of gestation. The blood pressure of caudal artery was measured at the age of 16 weeks, the myocardial fibrosis was observed by Masson staining in tissue section, the microstructure of myocardium was observed by transmission electron microscope. Real-time fluorescent quantitative PCR was used to detect the expression level of gap junction protein (Connexin) CxP43 in myocardial tissue and microtubule-associated protein 1light chain (3BU) microtubule-associated protein 1light chain (3BX) in microtubule-associated protein 1light chain. The expression level of DNMT-1DNMT-1 mRNA in microtubule-associated protein 1 was detected by Western blot, and the expression of Cx43LC3BDNMT-1 protein was detected by Western blot. Results compared with the offspring of Con group, the blood pressure of rats in lipopolysaccharide group increased significantly at the age of 16 weeks, and the collagen deposition in myocardial tissue increased and the gap junction between cells decreased. The expression level of Cx43 and DNMT-1 mRNA in autophagy bodies increased significantly, while the mRNA expression level of LC3B decreased significantly compared with that of the progenies of Con group. The expression levels of Cx3B 鈪，

本文編號：2003878