本文選題：酒精 + 心肌損傷�。� 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的探討脂聯(lián)素(APN)對小鼠急性酒精性心肌損傷的影響并研究相關(guān)機制,為急性酒精性心肌損傷及酒精性心肌病的預(yù)防和診治提供實驗依據(jù)及新思路。方法取20只健康8周齡SPF級C57BL/6J雄性小鼠,隨機分為正常實驗組(n=10只)與正常模型組(n=10只)。將8周齡純合子脂聯(lián)素基因敲除(APN-/-)雄性鼠(SPF級、近交系C57BL/6J,20只)隨機分為APN-/-對照組(n=10只)與APN-/-模型組(n=10只);各模型組均給予腹腔注射乙醇3g/Kg(體重)·d,各對照組均給予腹腔注射相等量的生理鹽水。4組小鼠均正常攝食、飲水,連續(xù)3天后進行各項指標(biāo)檢測:觀察小鼠一般情況;用動物超聲機及小動物專用探頭測定心臟結(jié)構(gòu)參數(shù)及相關(guān)功能指標(biāo);檢測血清乳酸脫氫酶(LDH)、血漿N尾端B型腦鈉肽原(NT-proBNP);檢測心肌組織Casepase-3活力及TUNEL檢測心肌組織細胞凋亡;制作心肌組織HE染色及Masson染色切片;制備心肌勻漿,測定MDA、ROS含量及SOD活性;Western blot法測定心肌組織Nox2蛋白表達情況。結(jié)果與正常對照組相比,正常模型組射血分數(shù)顯著下降(P0.01);血清LDH、血漿NT-proBNP濃度分別升高了1.98倍、5.13倍(均P0.01);HE染色及Masson染色結(jié)果示心肌結(jié)構(gòu)紊亂、心肌纖維走向迂曲、斷裂,膠原纖維增多,CVF值升高2.63倍(P0.01);心肌組織內(nèi)Casepase-3活性升高了2.58倍(P0.01),TUNEL法示陽性凋亡心肌細胞增多了12.67倍(P0.01);心肌組織內(nèi)ROS含量、MDA含量分別升高了1.68倍、2.87倍(均P0.01),SOD活性升高2.92倍(P0.01);Nox2蛋白表達量升高1.87倍(P0.01)。與APN-/-對照組比較,APN-/-模型組射血分數(shù)顯著下降(P0.01),其余指標(biāo)均顯著升高(均P0.01)。與正常模型組比較,APN-/-模型組小鼠射血分數(shù)降低更為顯著(P0.01),血清LDH、NT-proBNP濃度分別升高了1.30倍、1.25倍(均P0.01);HE染色及Masson染色示心肌結(jié)構(gòu)紊亂無序、心肌纖維走向迂曲、斷裂更加明顯、膠原纖維增多明顯,CVF值升高了1.55倍(P0.01);心肌組織內(nèi)Casepase-3活性升高1.66倍(P0.01),TUNEL法示陽性凋亡心肌細胞比例升高1.64倍(P0.01);心肌組織內(nèi)ROS含量、MDA含量分別升高1.42倍、1.39倍(均P0.01),SOD活性降低28%(P0.01),Nox2蛋白表達量升高1.44倍(P0.01)。結(jié)論脂聯(lián)素(APN)可以減輕小鼠急性酒精性心肌損傷,其機制與脂聯(lián)素抑制心肌組織Nox2表達發(fā)揮抗氧化應(yīng)激作用有關(guān)。
[Abstract]:Objective to investigate the effect of APN on acute alcoholic myocardial injury in mice and its related mechanism, and to provide experimental basis and new ideas for the prevention and treatment of acute alcoholic myocardial injury and alcoholic cardiomyopathy. Methods Twenty healthy 8-week-old SPF grade C57BL/6J male mice were randomly divided into normal experimental group (n = 10) and normal model group (n = 10). The 8 week old homozygous adiponectin gene knockout APN / -) male mice with SPF grade, Inbred line C57BL / 6 JJ (20) were randomly divided into APN-r-control group (n = 10) and APN-r-model group (n = 10). Each model group was given ethanol 3 g / Kg (body weight) d, and each control group was given a normal diet and drinking water of the same amount of normal saline. After 3 days of continuous testing, the general situation of mice was observed, the parameters of heart structure and related functional indexes were measured by animal ultrasound machine and small animal special probe. Serum lactate dehydrogenase (LDH), plasma N-tail B-type brain natriuretic peptide (NT-proBNPN), myocardial tissue Casepase-3 activity and TUNEL detection of myocardial apoptosis, myocardial HE staining and Masson staining sections were made, and myocardial homogenate was prepared. The content of MDAA Ros and the expression of Nox2 protein in myocardium were determined by Western blot. Results compared with the normal control group, the ejection fraction of the normal model group decreased significantly (P 0.01), the serum LDH and plasma NT-proBNP concentration increased by 1.98 times and 5.13 times respectively (P 0.01 he staining and Masson staining showed that the myocardial structure was disordered, the myocardial fibers were tortuous and broken. The Casepase-3 activity in myocardial tissue increased by 2.58 times and the number of positive apoptotic cardiomyocytes increased by 12.67 times by Tunel method. The content of ROS in myocardial tissue was increased by 1.68 times and 2.87 times respectively (all P0.01SOD activity increased). The protein expression of Nox2 was 1.87 times higher than that of P0.01. Compared with the control group, the ejection fraction of the APN-p-model group decreased significantly (P 0.01), and the other indexes increased significantly (P 0.01). Compared with the normal model group, the ejection fraction decreased significantly in the APN-r-model group, and the serum LDHNT-proBNP concentration increased by 1.30 times and 1.25 times respectively (P 0.01 he staining and Masson staining showed that the myocardial structure was disordered, the myocardial fiber direction was tortuous, and the rupture was more obvious. Casepase-3 activity in myocardial tissue increased by 1.66 times and the percentage of positive apoptotic cardiomyocytes was increased by 1.64 times by Tunel method. The content of ROS in myocardial tissue was increased by 1.42 times and 1.39 times respectively (all P0.01SOD activity). The expression of P0.01Nox2 protein increased by 1.44 times. Conclusion adiponectin (APN) can attenuate acute alcoholic myocardial injury in mice, and its mechanism is related to the antioxidation stress effect of adiponectin on the expression of Nox2 in myocardium.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R542.2

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相關(guān)期刊論文 前1條

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本文編號：1939789