本文選題：心肌缺血再灌注損傷 + 異丙腎上腺素��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:探究異丙腎上腺素作用于大鼠心肌缺血再灌注損傷的具體步驟,討論相關(guān)可能機(jī)理。方法:SD大鼠32只,雄性,予以分為四組,假手術(shù)組(Sham組)、缺血再灌注組(I/R組)、異丙腎上腺素預(yù)處理組(ISO組)和選擇性β2腎上腺素能受體(β2-adrenergic recepter,β2-AR)拮抗劑ICI118551+ISO-I/R組(ICI組)(隨機(jī)數(shù)字法),每組8只。繼大鼠左冠狀動脈前降支(LAD)血流中斷30min之后,予血液再灌流2h以構(gòu)建成心肌缺血再灌注損傷的大鼠實(shí)驗(yàn)?zāi)Ｐ汀ＬK木素-伊紅染色法觀測大鼠心肌組織形態(tài)學(xué),蛋白印記雜交技術(shù)用于檢出磷酸化絲/蘇氨酸激酶(P-Akt)的表達(dá)高低,原位末端凋亡法及免疫組織化學(xué)方法分別對心肌凋亡率及凋亡相關(guān)成分Caspase-3的表達(dá)進(jìn)行檢出,血清中代表氧化應(yīng)激高低的指標(biāo)超氧化物歧化酶(SOD)活性、丙二醛(MDA)濃度分別經(jīng)黃嘌呤氧化酶法、硫代巴比妥酸法檢出。結(jié)果:ISO組與I/R組比較發(fā)現(xiàn),前者大鼠心肌損害減輕明顯,P-Akt的表達(dá)顯著增多、心肌纖維凋亡率大大減低、Caspase-3的表達(dá)減弱突出、血清SOD活性增進(jìn)、血清MDA濃度減低且均具顯著性(P0.05);而ICI組與ISO組相比則提示,前者大鼠心肌損害較嚴(yán)重,心肌纖維P-Akt蛋白成分減少明顯、細(xì)胞凋亡率大幅度增高、Caspase-3表達(dá)明顯增強(qiáng)、血清SOD活性減弱、血清MDA濃度增高且均具有顯著性(P0.05)。結(jié)論:異丙腎上腺素可對大鼠心肌缺血再灌注損傷發(fā)揮有利的保護(hù)功能,具體機(jī)理可能與刺激β2-AR及PI3K-Akt信號轉(zhuǎn)導(dǎo)通道密切相關(guān)。
[Abstract]:Aim: to investigate the specific steps of isoproterenol on myocardial ischemia reperfusion injury in rats and discuss the possible mechanism. Methods Thirty-two male SD rats were divided into four groups. Sham group, I / R group, isoproterenol preconditioning group and ICI118551 ISO-I/R group (8 rats in each group). After the left anterior descending coronary artery (lad) blood flow was interrupted by 30min, the rat model of myocardial ischemia-reperfusion injury was established by blood reperfusion for 2 hours. The expression of phosphorylated silk / threonine kinase P-Akt was detected by hematoxylin-eosin staining and protein blot hybridization. Myocardial apoptosis rate and expression of apoptosis-related component Caspase-3 were detected by in situ terminal apoptosis method and immunohistochemical method respectively. The activity of superoxide dismutase (SOD), an indicator of oxidative stress, was detected in serum. Malondialdehyde (MDA) concentration was detected by xanthine oxidase method and thiobarbituric acid method. Results compared with I / R group, the expression of P-Akt was significantly increased, the expression of Caspase-3 was significantly decreased and the activity of serum SOD was increased. Compared with the ISO group, the serum MDA concentration in the ICI group was significantly lower than that in the ISO group, which indicated that the former group had more serious myocardial damage, the myocardial fiber P-Akt protein component was significantly decreased, and the apoptotic rate was significantly increased and the expression of Caspase-3 was significantly increased in the ICI group compared with the ISO group. The activity of serum SOD was decreased and the concentration of serum MDA was increased. Conclusion: isoproterenol may play a protective role in myocardial ischemia-reperfusion injury in rats, and the mechanism may be closely related to the stimulation of 尾 2-AR and PI3K-Akt signal transduction channels.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R541.4

【參考文獻(xiàn)】

相關(guān)期刊論文 前8條

1 王勇;范書英;;冠心病介入治療的現(xiàn)狀和展望[J];臨床內(nèi)科雜志;2015年01期

2 王雨晴;吳艷娜;李欣;尹永強(qiáng);康毅;婁建石;溫克;;RISK信號通路在1-磷酸鞘氨醇后適應(yīng)減輕心肌細(xì)胞缺氧/復(fù)氧損傷中的作用[J];中國藥理學(xué)通報;2015年02期

3 喬興科;石蘊(yùn)琦;袁龍;侯愛潔;王永新;李占全;;脂聯(lián)素、白細(xì)胞介素-18預(yù)測冠脈支架術(shù)后再狹窄血管重塑的價值[J];實(shí)用醫(yī)學(xué)雜志;2014年15期

4 王繼春;胡笑容;謝菁;周曉亞;江洪;;異丙腎上腺素對大鼠心肌缺血再灌注損傷中高遷移率族蛋白1表達(dá)的影響及機(jī)制[J];中華老年心腦血管病雜志;2014年03期

5 黃靚;張弛;王漢群;向瓊;佘美華;何平平;尹衛(wèi)東;;新型褪黑素受體激動劑Neu-p11對缺血/再灌注損傷大鼠心肌細(xì)胞凋亡的影響[J];中國藥理學(xué)通報;2013年12期

6 秦玉鳳;錢磊;李天鵬;韓磊;楊廷桐;;兔心肌缺血再灌注對其交界區(qū)細(xì)胞凋亡的影響及意義[J];實(shí)用醫(yī)藥雜志;2013年06期

7 杜霞;袁洪;邢曉為;;DNA甲基化與原發(fā)性高血壓的研究進(jìn)展[J];生物化學(xué)與生物物理進(jìn)展;2010年04期

8 謝剛,涂遠(yuǎn)超,黃從新,梁遠(yuǎn)紅;β_1腎上腺素能受體反義寡核苷酸對自發(fā)性高血壓大鼠心肌間質(zhì)及左室功能的影響[J];高血壓雜志;2005年02期

，

本文編號：1936127